LOCUS       V008579                 6256 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008579
VERSION     V008579
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ellermeier CD, Janakiraman A, Slauch JM.
  TITLE     Construction of targeted single copy lac fusions using lambda Red
            and FLP-mediated site-specific recombination in bacteria
  JOURNAL   Gene 290 (1-2), 153-161 (2002)
   PUBMED   12062810
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Ellermeier CD, Janakiraman A, Slauch JM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2001) Microbiology, University of Illinois
            Urbana-Champaign, 601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801,
            USA
REFERENCE   3  (bases 1 to 6256)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6256)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 290
            (1-2), 153-161 (2002)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2001) Microbiology, University of Illinois Urbana-Champaign,
            601 S. Goodwin Ave. B103 CLSL, Urbana, IL 61801, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             134..3205
                     /label="lacZ"
                     /note="beta-galactosidase"
     CDS             3260..4510
                     /gene="lacY"
                     /label="Lactose permease"
                     /note="Lactose permease from Escherichia coli (strain K12).
                     Accession#: P02920"
     regulatory      4719..4748
                     /note="histidine bi-directional rho-independent terminator
                     from Salmonella typhimurium"
                     /regulatory_class="terminator"
     CDS             complement(4999..5811)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      5981..6256
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 agcttttaag gtttaacggt tgtggacaac aagccaggga tgtaacgcac tgagaagccc
       61 ttagagcctc tcaaagcaat tttgagtgac acaggaacac ttaacggctg acatgggggg
      121 gtaccaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga
      181 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      241 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      301 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
      361 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta
      421 cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc cgtttgttcc
      481 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
      541 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa
      601 cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc
      661 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga gtgacggcag
      721 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct
      781 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt
      841 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg
      901 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
      961 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt
     1021 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact
     1081 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
     1141 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa
     1201 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
     1261 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
     1321 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat
     1381 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc
     1441 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
     1501 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat
     1561 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc
     1621 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt
     1681 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct
     1741 ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc
     1801 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct
     1861 gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac
     1921 gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc
     1981 agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac
     2041 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat
     2101 ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca
     2161 aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg
     2221 gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag
     2281 cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca
     2341 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg
     2401 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca
     2461 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt
     2521 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca
     2581 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct
     2641 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac
     2701 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga
     2761 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt
     2821 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt
     2881 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa
     2941 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt
     3001 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct
     3061 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg
     3121 cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg gtcgctacca
     3181 ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc tgcccgtatt
     3241 tcgcgtaagg aaatccatta tgtactattt aaaaaacaca aacttttgga tgttcggttt
     3301 attctttttc ttttactttt ttatcatggg agcctacttc ccgtttttcc cgatttggct
     3361 acatgacatc aaccatatca gcaaaagtga tacgggtatt atttttgccg ctatttctct
     3421 gttctcgcta ttattccaac cgctgtttgg tctgctttct gacaaactcg ggctgcgcaa
     3481 atacctgctg tggattatta ccggcatgtt agtgatgttt gcgccgttct ttatttttat
     3541 cttcgggcca ctgttacaat acaacatttt agtaggatcg attgttggtg gtatttatct
     3601 aggcttttgt tttaacgccg gtgcgccagc agtagaggca tttattgaga aagtcagccg
     3661 tcgcagtaat ttcgaatttg gtcgcgcgcg gatgtttggc tgtgttggct gggcgctgtg
     3721 tgcctcgatt gtcggcatca tgttcaccat caataatcag tttgttttct ggctgggctc
     3781 tggctgtgca ctcatcctcg ccgttttact ctttttcgcc aaaacggatg cgccctcttc
     3841 tgccacggtt gccaatgcgg taggtgccaa ccattcggca tttagcctta agctggcact
     3901 ggaactgttc agacagccaa aactgtggtt tttgtcactg tatgttattg gcgtttcctg
     3961 cacctacgat gtttttgacc aacagtttgc taatttcttt acttcgttct ttgctaccgg
     4021 tgaacagggt acgcgggtat ttggctacgt aacgacaatg ggcgaattac ttaacgcctc
     4081 gattatgttc tttgcgccac tgatcattaa tcgcatcggt gggaaaaacg ccctgctgct
     4141 ggctggcact attatgtctg tacgtattat tggctcatcg ttcgccacct cagcgctgga
     4201 agtggttatt ctgaaaacgc tgcatatgtt tgaagtaccg ttcctgctgg tgggctgctt
     4261 taaatatatt accagccagt ttgaagtgcg tttttcagcg acgatttatc tggtctgttt
     4321 ctgcttcttt aagcaactgg cgatgatttt tatgtctgta ctggcgggca atatgtatga
     4381 aagcatcggt ttccagggcg cttatctggt gctgggtctg gtggcgctgg gcttcacctt
     4441 aatttccgtg ttcacgctta gcggccccgg cccgctttcc ctgctgcgtc gtcaggtgaa
     4501 tgaagtcgct taagcaatca atgtcggatg cggcgcgacg cttatccgac caacatatca
     4561 taacggagtg atcgcattga acatgccaat gaccgaaaga ataagagcag gcaagctatt
     4621 taccgatatg tgcgaaggct taccggaaaa aagacttcgt gggaaaagca tgcgatttaa
     4681 gcaatcaatg tcggatgcgg cgcgacgctt atccgaccaa aaaaaccggg caatgcccgg
     4741 ttttttttaa ttattcctga atcgccccat catccagcca gaaagtgagg gagccacggt
     4801 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg
     4861 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat
     4921 ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca
     4981 attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     5041 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     5101 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     5161 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     5221 accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac
     5281 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     5341 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
     5401 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     5461 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
     5521 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     5581 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     5641 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
     5701 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     5761 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct
     5821 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg
     5881 tgcaatgtaa catcagagat tttgagacac aacgtggctt tccccccccc ccccgagctc
     5941 atcgatttcg aaccccggcc acgatgcgtc cggcgtagag gatctgaaga tcagcagttc
     6001 aacctgttga tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac
     6061 gtactaagct ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg
     6121 gctaacgtac taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt
     6181 aatataaatc agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa
     6241 tatataaggc ttttaa
//
