LOCUS       Exported                9696 bp DNA     circular SYN 03-DEC-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9696)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      1301..3090
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(3392..5317)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    5796..5817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5832..5862
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5870..5886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5894..5910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6271..6326
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(6480..6788)
                     /codon_start=1
                     /product="mouse p53(NM_011640) Delta-40-903, p53 
                     carboxy-terminal dominant-negative fragment."
                     /label=mp53DD
                     /translation="MTAMEESQSDISLKRALPTCTSASPPQKKKPLDGEYFTLKIRGRK
                     RFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD"
     intron          6856..7872
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     promoter        complement(7873..8149)
                     /label=chicken beta-actin promoter
     enhancer        8151..8530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8561..8665
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8666..9526
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccgctagcgt
      841 cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga catctctgcc
      901 ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact tgccaactgg
      961 gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc tgactgtagt
     1021 tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc atcctggagc
     1081 agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact cttggctgaa
     1141 gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga ctacgggagg
     1201 ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc ctcaagaggg
     1261 cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc atatgcttcc
     1321 cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt tacccaacgg
     1381 gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc gatatctccc
     1441 accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag ggtagtgaac
     1501 cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac tctcctgaat
     1561 cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg agttgtgaac
     1621 agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc agaataaaat
     1681 ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc tcacaaaccc
     1741 cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa caatagaaat
     1801 ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat ttatggctat
     1861 gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg tcccaggcag
     1921 ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga aagagagtgg
     1981 acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt aaacggggct
     2041 ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg aaattgtgga
     2101 gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta aaataagggt
     2161 gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact tgcccacaaa
     2221 accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca agataggggc
     2281 gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc caagcacagg
     2341 gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg ttgccatggg
     2401 tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc
     2461 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     2521 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     2581 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     2641 atatgctatc ctaatagaga ttagggtagt atatgctatc ctaatttata tctgggtagc
     2701 atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg ggtagcatat
     2761 gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat
     2821 gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag
     2881 gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat
     2941 gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca gtcagcatat
     3001 gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct cccaaggggg
     3061 cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag gtgaatttaa
     3121 ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt cgctaatgtt
     3181 ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg ggtatgccca
     3241 attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa atacaccaac
     3301 agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca cggcttttaa
     3361 tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca ccctcatctc
     3421 catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc ccttccacca
     3481 taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca gtcaccctga
     3541 tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc ttcaaaacct
     3601 cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac tcccttagcg
     3661 ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc aatggtgtaa
     3721 gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg gaggtcttac
     3781 tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc ctttctacgt
     3841 gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc gggttggaac
     3901 ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct ccatcaccct
     3961 gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc tgctctatcg
     4021 ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat tcaaaataat
     4081 cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg cgcggtggag
     4141 acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca cgtccacgac
     4201 ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct ctaccccggc
     4261 ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg cctccactgc
     4321 ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc cctcctcctg
     4381 ctcctgcccc tcctgcccct cctgctcctg cccctcctgc ccctcctgct cctgcccctc
     4441 ctgcccctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc
     4501 ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg
     4561 cccctcctgc ccctcctgct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc
     4621 ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgctcctg
     4681 cccctcctgc ccctcctgcc cctcctgctc ctgcccctcc tcctgctcct gcccctcctg
     4741 cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct cctgcccctc
     4801 ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc cctcctgccc
     4861 ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctc
     4921 ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctgcccctc
     4981 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
     5041 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
     5101 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
     5161 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
     5221 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
     5281 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
     5341 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
     5401 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
     5461 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
     5521 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
     5581 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
     5641 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
     5701 cagcttatcg atgataagct gtcaaacatg agaattgaga tccgctggca cgacaggttt
     5761 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5821 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5881 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg gctgcaggtc
     5941 gagggatctc cataagagaa gagggacagc tatgactggg agtagtcagg agaggaggaa
     6001 aaatctggct agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa aaaataacac
     6061 aaaacaaaat ataaaaaaaa tctaacctca agtcaaggct tttctatgga ataaggaatg
     6121 gacagcaggg ggctgtttca tatactgatg acctctttat agccaacctt tgttcatggc
     6181 agccagcata tgggcatatg ttgccaaact ctaaaccaaa tactcattct gatgttttaa
     6241 atgatttgcc ctcccatatg tccttccgag tgagagacac aaaaaattcc aacacactat
     6301 tgcaatgaaa ataaatttcc tttattagcc agaagtcaga tgctcaaggg gcttcatgat
     6361 gtccccataa tttttggcag agggaaaaag atctcagtgg tatttgtgag ccagggcatt
     6421 ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtga attcgccctt
     6481 cagtctgagt caggccccac tttcttgacc attgtttttt tatggcggga agtagactgg
     6541 cccttcttgg tcttcaggta gctggagtga gccctgctgt ctccagactc ctctgtagca
     6601 tgggcatcct ttaactctaa ggcctcattc agctcccgga acatctcgaa gcgtttacgc
     6661 ccgcggatct tgagggtgaa atactctcca tcaagtggtt ttttcttttg cgggggagag
     6721 gcgcttgtgc aggtgggcag cgctctcttg aggctgatat ccgactgtga ctcctccatg
     6781 gcagtcatcc tggtgaaggg cgaattcttt gccaaaatga tgagacagca caataaccag
     6841 cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct
     6901 agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca
     6961 gccgtccccc cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga
     7021 cgcacgaagg ccctccccgc ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg
     7081 cccgctagag ggggtgcggc ggcgcctccc agatttcggc tccgcacaga tttgggacaa
     7141 aggaagtccc tgcgccctct cgcacgatta ccataaaagg caatggctgc ggctcgccgc
     7201 gcctcgacag ccgccggcgc tccgggggcc gccgcgcccc tcccccgagc cctccccggc
     7261 ccgaggcggc cccgccccgc ccggcacccc cacctgccgc caccccccgc ccggcacggc
     7321 gagccccgcg ccacgccccg tacggagccc cgcacccgaa gccgggccgt gctcagcaac
     7381 tcggggaggg gggtgcaggg ggggttgcag cccgaccgac gcgcccacac cccctgctca
     7441 cccccccacg cacacacccc gcacgcagcc tttgttcccc tcgcagcccc ccccgcaccg
     7501 cggggcaccg cccccggccg cgctcccctc gcgcacactg cggagcgcac aaagccccgc
     7561 gccgcgcccg cagcgctcac agccgccggg cagcgcggag ccgcacgcgg cgctccccac
     7621 gcacacacac acgcacgcac cccccgagcc gctccccccg cacaaagggc cctcccggag
     7681 cccctcaagg ctttcacgca gccacagaaa agaaacaagc cgtcattaaa ccaagcgcta
     7741 attacagccc ggaggagaag ggccgtcccg cccgctcacc tgtgggagta acgcggtcag
     7801 tcagagccgg ggcgggcggc gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg
     7861 cgcagcgact cccgcccgcc gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct
     7921 cgccataaaa ggaaactttc ggagcgcgcc gctctgattg gctgccgccg cacctctccg
     7981 cctcgccccg ccccgcccct cgccccgccc cgccccgcct ggcgcgcgcc cccccccccc
     8041 cccgccccca tcgctgcaca aaataattaa aaaataaata aatacaaaat tgggggtggg
     8101 gagggggggg agatggggag agtgaagcag aacgtggggc tcacctcgac catggtaata
     8161 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     8221 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     8281 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     8341 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     8401 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     8461 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
     8521 aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     8581 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     8641 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     8701 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     8761 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8821 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8881 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8941 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9001 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9061 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9121 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9181 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     9241 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9301 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9361 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9421 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9481 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     9541 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     9601 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     9661 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
