LOCUS       pCE-hSK       11876 bp DNA     circular SYN 20-JUL-2025
DEFINITION  Non-integrating (episomal) expression of human SOX2 and KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 11876)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11876)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..658
                     /label=chicken beta-actin promoter
     intron          659..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids,
                     forward primer"
     CDS             1740..2690
                     /codon_start=1
                     /label=hSOX2
                     /note="Homo sapiens transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of 
                     transcription factors, which is involved in the regulation 
                     of embryonic development and in the determination of cell 
                     fate"
                     /translation="MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPM
                     NAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKE
                     HPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHM
                     NGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPT
                     YSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGA
                     EVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM"
     CDS             2715..2780
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             2805..4214
                     /codon_start=1
                     /label=hKLF4
                     /note="Homo sapiens Kruppel-like factor 4 (Klf4) gene.
                     Belongs to the relatively large family of SP1-like 
                     transcription factors and is involved in the regulation of 
                     proliferation, differentiation, apoptosis and somatic cell 
                     reprogramming."
                     /translation="MAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRL
                     PPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSL
                     THPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLL
                     YGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVL
                     KASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGA
                     GPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYP
                     SFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYT
                     KSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSR
                     SDHLALHMKRHF"
     primer_bind     complement(4320..4339)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4385..4440
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4439..4458)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also
                     called rb-glob-pA-term-R"
     primer_bind     complement(4801..4817)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4801..4817)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4814..4836)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4825..4841
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4849..4879)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4894..4915)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             5394..7316
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      7621..9410
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(9951..9968)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10122..10710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10881..11741)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11504..11523
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11742..11846)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcg cccttcacca
     1741 tgtacaacat gatggagacg gagctgaagc cgccgggccc gcagcaaact tcggggggcg
     1801 gcggcggcaa ctccaccgcg gcggcggccg gcggcaacca gaaaaacagc ccggaccgcg
     1861 tcaagcggcc catgaatgcc ttcatggtgt ggtcccgcgg gcagcggcgc aagatggccc
     1921 aggagaaccc caagatgcac aactcggaga tcagcaagcg cctgggcgcc gagtggaaac
     1981 ttttgtcgga gacggagaag cggccgttca tcgacgaggc taagcggctg cgagcgctgc
     2041 acatgaagga gcacccggat tataaatacc ggccccggcg gaaaaccaag acgctcatga
     2101 agaaggataa gtacacgctg cccggcgggc tgctggcccc cggcggcaat agcatggcga
     2161 gcggggtcgg ggtgggcgcc ggcctgggcg cgggcgtgaa ccagcgcatg gacagttacg
     2221 cgcacatgaa cggctggagc aacggcagct acagcatgat gcaggaccag ctgggctacc
     2281 cgcagcaccc gggcctcaat gcgcacggcg cagcgcagat gcagcccatg caccgctacg
     2341 acgtgagcgc cctgcagtac aactccatga ccagctcgca gacctacatg aacggctcgc
     2401 ccacctacag catgtcctac tcgcagcagg gcacccctgg catggctctt ggctccatgg
     2461 gttcggtggt caagtccgag gccagctcca gcccccctgt ggttacctct tcctcccact
     2521 ccagggcgcc ctgccaggcc ggggacctcc gggacatgat cagcatgtat ctccccggcg
     2581 ccgaggtgcc ggaacccgcc gcccccagca gacttcacat gtcccagcac taccagagcg
     2641 gcccggtgcc cggcacggcc attaacggca cactgcccct ctcacacatg ggggatctca
     2701 aaattgtcgc tcctgtcaaa caaactctta actttgattt actcaaactg gctggggatg
     2761 tagaaagcaa tccaggtcca ctcatgaaca attcgccctt caccatggct gtcagtgacg
     2821 cgctgctccc atctttctcc acgttcgcgt ctggcccggc gggaagggag aagacactgc
     2881 gtcaagcagg tgccccgaat aaccgctggc gggaggagct ctcccacatg aagcgacttc
     2941 ccccagtgct tcccggccgc ccctatgacc tggcggcggc gaccgtggcc acagacctgg
     3001 agagcggcgg agccggtgcg gcttgcggcg gtagcaacct ggcgccccta cctcggagag
     3061 agaccgagga gttcaacgat ctcctggacc tggactttat tctctccaat tcgctgaccc
     3121 atcctccgga gtcagtggcc gccaccgtgt cctcgtcagc gtcagcctcc tcttcgtcgt
     3181 cgccgtcgag cagcggccct gccagcgcgc cctccacctg cagcttcacc tatccgatcc
     3241 gggccgggaa cgacccgggc gtggcgccgg gcggcacggg cggaggcctc ctctatggca
     3301 gggagtccgc tccccctccg acggctccct tcaacctggc ggacatcaac gacgtgagcc
     3361 cctcgggcgg cttcgtggcc gagctcctgc ggccagaatt ggacccggtg tacattccgc
     3421 cgcagcagcc gcagccgcca ggtggcgggc tgatgggcaa gttcgtgctg aaggcgtcgc
     3481 tgagcgcccc tggcagcgag tacggcagcc cgtcggtcat cagcgtcagc aaaggcagcc
     3541 ctgacggcag ccacccggtg gtggtggcgc cctacaacgg cgggccgccg cgcacgtgcc
     3601 ccaagatcaa gcaggaggcg gtctcttcgt gcacccactt gggcgctgga ccccctctca
     3661 gcaatggcca ccggccggct gcacacgact tccccctggg gcggcagctc cccagcagga
     3721 ctaccccgac cctgggtctt gaggaagtgc tgagcagcag ggactgtcac cctgccctgc
     3781 cgcttcctcc cggcttccat ccccacccgg ggcccaatta cccatccttc ctgcccgatc
     3841 agatgcagcc gcaagtcccg ccgctccatt accaagagct catgccaccc ggttcctgca
     3901 tgccagagga gcccaagcca aagaggggaa gacgatcgtg gccccggaaa aggaccgcca
     3961 cccacacttg tgattacgcg ggctgcggca aaacctacac aaagagttcc catctcaagg
     4021 cacacctgcg aacccacaca ggtgagaaac cttaccactg tgactgggac ggctgtggat
     4081 ggaaattcgc ccgctcagat gaactgacca ggcactaccg taaacacacg gggcaccgcc
     4141 cgttccagtg ccaaaaatgc gaccgagcat tttccaggtc ggaccacctc gccttacaca
     4201 tgaagagaca tttttaaaag ggcgaattgc ctgcaggaat tcactcctca ggtgcaggct
     4261 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     4321 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     4381 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     4441 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     4501 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     4561 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     4621 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     4681 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     4741 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     4801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tctcaattct
     4981 catgtttgac agcttatcat cgataagctg atcctcacag gccgcaccca gcttttcttc
     5041 cgttgcccca gtagcatctc tgtctggtga ccttgaagag gaagaggagg ggtcccgaga
     5101 atccccatcc ctaccgtcca gcaaaaaggg ggacgaggaa tttgaggcct ggcttgaggc
     5161 tcaggacgca aatcttgagg atgttcagcg ggagttttcc gggctgcgag taattggtga
     5221 tgaggacgag gatggttcgg aggatgggga attttcagac ctggatctgt ctgacagcga
     5281 ccatgaaggg gatgagggtg ggggggctgt tggagggggc aggagtctgc actccctgta
     5341 ttcactgagc gtcgtctaat aaagatgtct attgatctct tttagtgtga atcatgtctg
     5401 acgaggggcc aggtacagga cctggaaatg gcctaggaga gaagggagac acatctggac
     5461 cagaaggctc cggcggcagt ggacctcaaa gaagaggggg tgataaccat ggacgaggac
     5521 ggggaagagg acgaggacga ggaggcggaa gaccaggagc cccgggcggc tcaggatcag
     5581 ggccaagaca tagagatggt gtccggagac cccaaaaacg tccaagttgc attggctgca
     5641 aagggaccca cggtggaaca ggagcaggag caggagcggg aggggcagga gcaggagggg
     5701 caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag
     5761 gggcaggagc aggaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag
     5821 gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggagca ggaggagggg
     5881 caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga ggggcaggag
     5941 gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag
     6001 gagcaggagg ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggaggag
     6061 gggcaggagg ggcaggaggg gcaggagcag gaggggcagg agcaggaggg gcaggagcag
     6121 gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag
     6181 caggaggggc aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag
     6241 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg
     6301 caggagcagg aggggcagga ggggcaggag caggaggagg ggcaggagca ggaggggcag
     6361 gagcaggagg tggaggccgg ggtcgaggag gcagtggagg ccggggtcga ggaggtagtg
     6421 gaggccgggg tcgaggaggt agtggaggcc gccggggtag aggacgtgaa agagccaggg
     6481 ggggaagtcg tgaaagagcc agggggagag gtcgtggacg tggagaaaag aggcccagga
     6541 gtcccagtag tcagtcatca tcatccgggt ctccaccgcg caggccccct ccaggtagaa
     6601 ggccattttt ccaccctgta ggggaagccg attattttga ataccaccaa gaaggtggcc
     6661 cagatggtga gcctgacgtg cccccgggag cgatagagca gggccccgca gatgacccag
     6721 gagaaggccc aagcactgga ccccggggtc agggtgatgg aggcaggcgc aaaaaaggag
     6781 ggtggtttgg aaagcatcgt ggtcaaggag gttccaaccc gaaatttgag aacattgcag
     6841 aaggtttaag agctctcctg gctaggagtc acgtagaaag gactaccgac gaaggaactt
     6901 gggtcgccgg tgtgttcgta tatggaggta gtaagacctc cctttacaac ctaaggcgag
     6961 gaactgccct tgctattcca caatgtcgtc ttacaccatt gagtcgtctc ccctttggaa
     7021 tggcccctgg acccggccca caacctggcc cgctaaggga gtccattgtc tgttatttca
     7081 tggtcttttt acaaactcat atatttgctg aggttttgaa ggatgcgatt aaggaccttg
     7141 ttatgacaaa gcccgctcct acctgcaata tcagggtgac tgtgtgcagc tttgacgatg
     7201 gagtagattt gcctccctgg tttccaccta tggtggaagg ggctgccgcg gagggtgatg
     7261 acggagatga cggagatgaa ggaggtgatg gagatgaggg tgaggaaggg caggagtgat
     7321 gtaacttgtt aggagacgcc ctcaatcgta ttaaaagccg tgtattcccc cgcactaaag
     7381 aataaatccc cagtagacat catgcgtgct gttggtgtat ttctggccat ctgtcttgtc
     7441 accattttcg tcctcccaac atggggcaat tgggcatacc catgttgtca cgtcactcag
     7501 ctccgcgctc aacaccttct cgcgttggaa aacattagcg acatttacct ggtgagcaat
     7561 cagacatgcg acggctttag cctggcctcc ttaaattcac ctaagaatgg gagcaaccag
     7621 caggaaaagg acaagcagcg aaaattcacg cccccttggg aggtggcggc atatgcaaag
     7681 gatagcactc ccactctact actgggtatc atatgctgac tgtatatgca tgaggatagc
     7741 atatgctacc cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc
     7801 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc
     7861 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     7921 ctatgctacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     7981 atatgctatc cagatatttg ggtagtatat gctacccaga tataaattag gatagcatat
     8041 actaccctaa tctctattag gatagcatat gctacccgga tacagattag gatagcatat
     8101 actacccaga tatagattag gatagcatat gctacccaga tatagattag gatagcctat
     8161 gctacccaga tataaattag gatagcatat actacccaga tatagattag gatagcatat
     8221 gctacccaga tatagattag gatagcctat gctacccaga tatagattag gatagcatat
     8281 gctatccaga tatttgggta gtatatgcta cccatggcaa cattagccca ccgtgctctc
     8341 agcgacctcg tgaatatgag gaccaacaac cctgtgcttg gcgctcaggc gcaagtgtgt
     8401 gtaatttgtc ctccagatcg cagcaatcgc gcccctatct tggcccgccc acctacttat
     8461 gcaggtattc cccggggtgc cattagtggt tttgtgggca agtggtttga ccgcagtggt
     8521 tagcggggtt acaatcagcc aagttattac acccttattt tacagtccaa aaccgcaggg
     8581 cggcgtgtgg gggctgacgc gtgcccccac tccacaattt caaaaaaaag agtggccact
     8641 tgtctttgtt tatgggcccc attggcgtgg agccccgttt aattttcggg ggtgttagag
     8701 acaaccagtg gagtccgctg ctgtcggcgt ccactctctt tccccttgtt acaaatagag
     8761 tgtaacaaca tggttcacct gtcttggtcc ctgcctggga cacatcttaa taaccccagt
     8821 atcatattgc actaggatta tgtgttgccc atagccataa attcgtgtga gatggacatc
     8881 cagtctttac ggcttgtccc caccccatgg atttctattg ttaaagatat tcagaatgtt
     8941 tcattcctac actagtattt attgcccaag gggtttgtga gggttatatt ggtgtcatag
     9001 cacaatgcca ccactgaacc ccccgtccaa attttattct gggggcgtca cctgaaacct
     9061 tgttttcgag cacctcacat acaccttact gttcacaact cagcagttat tctattagct
     9121 aaacgaagga gaatgaagaa gcaggcgaag attcaggaga gttcactgcc cgctccttga
     9181 tcttcagcca ctgcccttgt gactaaaatg gttcactacc ctcgtggaat cctgacccca
     9241 tgtaaataaa accgtgacag ctcatggggt gggagatatc gctgttcctt aggacccttt
     9301 tactaaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
     9361 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttaac
     9421 aagggggcct tataaacact attgctaatg ccctcttgag ggtccgctta tcggtagcta
     9481 cacaggcccc tctgattgac gttggtgtag cctcccgtag tcttcctggg cccctgggag
     9541 gtacatgtcc cccagcattg gtgtaagagc ttcagccaag agttacacat aaaggcaatg
     9601 ttgtgttgca gtccacagac tgcaaagtct gctccaggat gaaagccact cagtgttggc
     9661 aaatgtgcac atccatttat aaggatgtca actacagtca gagaacccct ttgtgtttgg
     9721 tccccccccg tgtcacatgt ggaacagggc ccagttggca agttgtacca accaactgaa
     9781 gggattacat gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg
     9841 tccggggcgg ggatcgatcc tctagagtcg acgctagcgg atccgctgca ttaatgaatc
     9901 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9961 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    10021 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10081 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10141 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10201 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10261 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10321 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10381 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10441 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10501 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10561 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10621 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10681 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10741 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10801 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10861 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10921 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10981 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    11041 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11101 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11161 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11221 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11281 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11341 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11401 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11461 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11521 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11581 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11641 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11701 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11761 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11821 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtc
//
