LOCUS       V013534                10049 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013534
VERSION     V013534
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        253..632
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        633..836
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        1386..1404
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1427..5485
                     /gene="S"
                     /label="Spike glycoprotein"
                     /note="Spike glycoprotein from Middle East respiratory
                     syndrome-related coronavirus (isolate United
                     Kingdom/H123990006/2012). Accession#: K9N5Q8"
     CDS             5501..5524
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     polyA_signal    5587..5811
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5857..6285
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6299..6628
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             6677..7699
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    7832..7965
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(8050..8080)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8095..8116)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8404..8992)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9095..9907)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg agtacattta tattggctca tgtccaatat
      241 gaccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      301 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      361 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      421 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      481 tggcagtaca tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta
      541 aatggcccgc ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt
      601 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg
      661 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      721 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc
      781 cgttgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
      841 tagtgaaccg tcagatcctc actctcttcc gcatcgctgt ctgcgagggc cagctgttgg
      901 gctcgcggtt gaggacaaac tcttcgcggt ctttccagta ctcttggatc ggaaacccgt
      961 cggcctccga acggtactcc gccaccgagg gacctgagcg agtccgcatc gaccggatcg
     1021 gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc aaggtaggct gagcaccgtg
     1081 gcgggcggca gcgggtggcg gtcggggttg tttctggcgg aggtgctgct gatgatgtaa
     1141 ttaaagtagg cggtcttgag acggcggatg gtcgaggtga ggtgtggcag gcttgagatc
     1201 cagctgttgg ggtgagtact ccctctcaaa agcgggcatt acttctgcgc taagattgtc
     1261 agtttccaaa aacgaggagg atttgatatt cacctggccc gatctggcca tacacttgag
     1321 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaagttta
     1381 aactttaata cgactcacta taggggccgc caccaagctt ggtaccatga ttcactctgt
     1441 gttcctgctg atgttcctgc tgacaccaac agagtcctat gtggatgtgg gacctgactc
     1501 tgtgaagtct gcctgtattg aggtggacat ccaacagacc ttctttgaca agacctggcc
     1561 aagaccaatt gatgtgagca aggctgatgg catcatctac ccacagggca ggacctacag
     1621 caacatcacc atcacctacc agggactgtt tccataccag ggagatcatg gagatatgta
     1681 tgtctactct gctggtcatg ccacaggcac cacaccacag aaactgtttg tggctaacta
     1741 cagccaggat gtgaagcagt ttgccaatgg ctttgtggtg aggattggag cagcagccaa
     1801 cagcacaggc acagtgatta tcagcccaag cacctctgcc accatcagga agatttaccc
     1861 tgcctttatg ctgggctcct ctgtgggcaa cttctctgat ggcaagatgg gcaggttctt
     1921 caaccacacc ctggtgctgc tgcctgatgg ctgtggcacc ctgctgaggg ctttctactg
     1981 tatcttggaa ccaaggtctg gcaaccactg tcctgctggc aactcctaca cctcctttgc
     2041 cacctaccac acacctgcca cagactgttc tgatggcaac tacaacagga atgcctccct
     2101 gaactccttc aaggaatact tcaacctgag gaactgtacc tttatgtaca cctacaacat
     2161 cacagaggat gagattttgg agtggtttgg catcacccag acagcccagg gagtgcatct
     2221 gttctcgagc agatatgtgg acctctatgg aggcaatatg ttccagtttg ccaccctgcc
     2281 tgtctatgac accatcaaat actacagcat catcccacac agcatcagga gcatccagtc
     2341 tgacaggaag gcttgggctg ccttctatgt ctacaaactc caaccactga ccttcctgct
     2401 ggacttctct gtggatggct acatcaggag ggctattgac tgtggcttca atgacctgag
     2461 ccaacttcac tgttcctatg agtcctttga tgtggagtct ggagtctact ctgtgtcctc
     2521 ctttgaggct aagccatctg gctctgtggt ggaacaggct gagggagtgg agtgtgactt
     2581 cagcccactg ctgtctggca cacctccaca ggtctacaac ttcaagagac tggtgttcac
     2641 caactgtaac tacaacctga ccaaactgct gtccctgttc tctgtgaatg acttcacttg
     2701 tagccagatt agccctgctg ccattgccag caactgttac tcctccctga ttctggacta
     2761 cttctcctac ccactgagta tgaagtctga cctgtctgtg tcctctgctg gaccaatcag
     2821 ccagttcaac tacaagcagt ccttcagcaa cccaacttgt ctgattctgg ctacagtgcc
     2881 acacaacctg accaccatca ccaagccact gaaatactcc tacatcaaca agtgtagcag
     2941 actgctgtct gatgacagga cagaggtgcc acaactagtg aatgccaacc aatacagccc
     3001 atgtgtgagc attgtgccaa gcacagtgtg ggaggatgga gactactaca ggaagcaact
     3061 tagcccattg gagggaggag gctggctggt ggcatctggc agcacagtgg ctatgacaga
     3121 acaactccaa atgggctttg gcatcacagt ccaatatggc acagacacca actctgtgtg
     3181 tccaaaattg gagtttgcca atgacaccaa gattgccagc caacttggca actgtgtgga
     3241 atactccctc tatggagtgt ctggcagggg agtgttccag aactgtactg ctgtgggagt
     3301 gagacaacag aggtttgtct atgatgccta ccagaacctg gtgggctact actctgatga
     3361 tggcaactac tactgtctga gggcttgtgt gtctgtgcct gtgtctgtga tttatgacaa
     3421 ggagaccaag acccatgcca ccctgtttgg ctctgtggct tgtgaacaca tctccagcac
     3481 aatgagtcaa tacagcagga gcaccaggag tatgctgaaa aggagggaca gcacatatgg
     3541 accactccaa acacctgtgg gctgtgtgct gggactggtg aactcctccc tgtttgtgga
     3601 ggactgtaaa ctgccactgg gacaatccct gtgtgccctg cctgacacac caagcaccct
     3661 gacaccaagg tctgtgaggt ctgtgcctgg agagatgaga ctggcaagca ttgccttcaa
     3721 ccacccaatc caggtggacc aacttaactc ctcctacttc aaactgagca tcccaaccaa
     3781 cttctccttt ggagtgaccc aggaatacat ccagaccacc atccagaagg tgacagtgga
     3841 ctgtaagcaa tatgtgtgta atggcttcca gaagtgtgaa caacttctga gggaatatgg
     3901 acaattctgt agcaagataa accaggctct tcatggagcc aacctgagac aggatgactc
     3961 tgtgaggaac ctgtttgcct ctgtgaagtc cagccagtcc agcccaatca tccctggctt
     4021 tggaggagac ttcaacctga ccctgttgga accggtgagc atcagcacag gcagcaggtc
     4081 tgccaggtct gccattgagg acctgctgtt tgacaaggtg accattgctg accctggcta
     4141 tatgcagggc tatgatgact gtatgcaaca gggacctgcc tctgccaggg acctgatttg
     4201 tgcccaatat gtggctggct acaaggtgct gcctccactg atggatgtga atatggaggc
     4261 tgcctacacc tcctccctgc tgggcagcat tgctggagtg ggctggactg caggactgtc
     4321 ctcctttgct gccatcccat ttgcccagag catcttctac agactgaatg gagtgggcat
     4381 cacccaacag gtgctgtctg agaaccagaa actgattgcc aacaagttca accaggctct
     4441 gggagctatg cagacaggct tcaccaccac caatgaggct ttccagaagg tccaggatgc
     4501 tgtgaacaac aatgcccagg ctctgagcaa actggcatct gaactgagca acacctttgg
     4561 agccatctct gctagcattg gagacatcat ccagagactg gatgtgttgg aacaggatgc
     4621 ccagattgac agactgataa atggcagact gaccaccctg aatgcctttg tggctcaaca
     4681 acttgtgagg tctgagtctg ctgccctgtc tgcccaactt gccaaggaca aggtgaatga
     4741 gtgtgtgaag gctcaaagca agaggtctgg cttctgtgga caaggcaccc acattgtgtc
     4801 ctttgtggtg aatgccccaa atggactcta ctttatgcat gtgggctact acccaagcaa
     4861 ccacattgag gtggtgtctg cctatggact gtgtgatgct gccaacccaa ccaactgtat
     4921 tgcccctgtg aatggctact tcatcaagac caacaacacc aggattgtgg atgagtggtc
     4981 ctacacaggc tcctccttct atgcccctga accaatcacc tccctgaaca ccaaatatgt
     5041 ggctccacag gtgacctacc agaacatcag caccaacctg cctcctccac tgctgggcaa
     5101 cagcacaggc attgacttcc aggatgaact ggatgagttc ttcaagaatg tgagcaccag
     5161 catcccaaac tttggctccc tgacccagat aaacaccacc ctgctggacc tgacctatga
     5221 gatgctgtcc ctccaacagg tggtgaaggc tctgaatgag tcctacattg acctgaaaga
     5281 actgggcaac tacacctact acaacaagtg gccatggtac atctggctgg gcttcatcgc
     5341 tggcctggtg gccctggcgc tgtgcgtgtt cttcatcctg tgctgcaccg gctgcggcac
     5401 caactgcatg ggcaagctga agtgcaacag gtgctgcgac aggtacgagg agtacgacct
     5461 ggagccccac aaggtgcacg tacatggggg tggaggctct gattacaagg atgacgacga
     5521 taagtaaact cgagtctaga gcggccgccg aattcgggcc cgtttaaacc cgctgatcag
     5581 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5641 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5701 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5761 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5821 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5881 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5941 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgcaggcttt ccccgtcaag
     6001 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6061 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6121 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6181 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6241 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6301 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6361 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6421 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6541 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6601 cttttttgga ggcctaggct tttgcaaaaa gctctcggga gcttgtatat ccattttcgg
     6661 atctgatcag cacgtgatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     6721 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     6781 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     6841 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     6901 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     6961 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     7021 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     7081 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     7141 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     7201 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     7261 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     7321 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     7381 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     7441 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     7501 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     7561 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     7621 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     7681 tcgtccgagg gcaaaggaat agcacgtgct acgagatttc gattccaccg ccgccttcta
     7741 tgaaaggttg ggcttcggaa tcgttttccg ggacgctggc tggatgatcc tccagcgcgg
     7801 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7861 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7921 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     7981 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8041 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8821 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9001 tttgatcttt tctacggggt ctgagcgcgg aacccctatt tgtttatttt tctaaataca
     9061 ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa
     9121 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     9181 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     9241 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     9301 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     9361 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     9421 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     9481 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     9541 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     9601 cagggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     9661 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     9721 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     9781 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     9841 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat
     9901 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgacc
     9961 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aatccgcgca
    10021 catttccccg aaaagtgcca cctgacgtc
//
