LOCUS       V015190                 8278 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015190
VERSION     V015190
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             931..3765
                     /gene="Sfswap"
                     /label="Splicing factor, suppressor of white-apricot
                     homolog"
                     /note="Splicing factor, suppressor of white-apricot homolog
                     from Mus musculus. Accession#: Q3USH5"
     CDS             3781..3798
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    3845..4069
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4115..4543
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4557..4886
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4953..5744
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5921..6054
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6091..6107)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6115..6131)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6139..6169)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6184..6205)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6493..7078)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7252..8109)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8110..8214)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgtacggtg ccggcggggg ccgtgccaag
      961 ccggagagga aaggcggagt gaaggaggag gccgggccgg gcggcaccgg cactgggggc
     1021 aaccgggtgg agcttctggt tttcggctat gcctgcaagt tgttccgcga cgacgagcgg
     1081 gctttggccc aggaacaggg acagcacctc atcccctgga tgggggaccc caagatcctc
     1141 atcgacagat acgacgggcg tggtcacctg catgacctct ctgcgtacga tgctgagtac
     1201 gccacgtgga accgagacta ccagctgtct gaagaggagg ctcgggtaga ggcgctgtgt
     1261 gatgaagaga ggtatttagc cttgcatacg gacttgcttg aggaggaggc aaggcaagag
     1321 gaagaataca aaagattgag tgaagcgctg gcggaggatg gaaactacag tgccgtaggg
     1381 ttcacttacg gcagcgacta ttatgacccg tcagagccca cggaagagga ggagccttcg
     1441 aagcagagag aaaaaaatga ggctgaaaac ttggaagaaa atgaagaacc tttcatcgcc
     1501 cctttaggat taagcgtccc gtctgatgtg gagctgccac caacagccaa aatgcatgcc
     1561 atcattgagc gcacagccaa cttcgtatgc aagcagggag cacagttcga gatcatgctg
     1621 aaggccaagc aagctcgaaa ctcacagttc gacttcctac ggtttgatca ctacctcaac
     1681 ccctactata aattcatcca gaaagccatg aaagaaggac ggtacacggt gctagctgag
     1741 agcaagaacg aggagaagaa aaagtctggg ccaacctctg acaacgaaga ggaagatgat
     1801 gaggaagatg ggagttacct gcacccgtct ctctttgctt ccaagaagag cagccgcctg
     1861 gaagagctga tgaagccctt gaaggtggtg gacccggatc accctctagc agcccttgtc
     1921 cggaaagcac aagctgacag ctcagctcca gccccaccca ctgcagatgg cacacctgct
     1981 cagccctccc aggtggagta cacggcagac tcaactgtgg cagccatgta ctacagctac
     2041 tacatgttgc cagatggtac ctactgcctg gcaccacccc cacctgggat cgatgtggcc
     2101 acttactaca gcacccttcc tgctggagtg actgtgtcca gctcacctgg tgtgactacc
     2161 actgtaccac cgcctcctgg gaccacacct cctccacccc caaccacagc tgaaccaagc
     2221 agcggggtta cctccacaac caccaccaca agtgcgcttg ctcccgtggc catcataccc
     2281 ccgccccccg acatccagcc tgtgattgac aagctggcag agtacgtcgc tagaaacggc
     2341 cttaaatttg agaccagtgt tcgagccaag aacgatcaaa gatttgaatt tcttcagccc
     2401 tggcaccagt acaacgccta ctacgagttt aagaagcagt tcttcctgca gaaagaagga
     2461 ggcggtagca cacaggcagc atcaacagct gaagaggctc ccacagaaac cgctgttgaa
     2521 gagtcgggtg aagctggaga ggacggcgct cctgagggta tggcagagac tgggggaaga
     2581 ggcagcggga agaaggaggc tgggtccagc aagagcaccg tggacgggaa gctggtaaaa
     2641 gcttcttttg ctccaataag ctttgccatc aaagccaaag aaaatgacct ccttcccttg
     2701 gagaaaaacc gtgtcaagct ggacgatgac agtgaagagg atgaagaaag cagggagtgc
     2761 caggaaagca caagcagcgt ggccaacccc agccctgctg ccgccccgcc ctccgtggct
     2821 gtagaggaga agaaaccaca gcttacccag gaggagctgg aagccaagca agcaaaacaa
     2881 aagctggagg accgccttgc tgctgctgcc cgggaaaagc tagcccaggc gtccaaggaa
     2941 tcaaaggaga agcagcttca ggctgaacgt aaaaggaaag cagcgttgtt cttacaaacc
     3001 ttaaaaaatc ctcttccaga agcagaagtt ggaaaacttg aggagagtac tttcggggtt
     3061 gaggacactg gtgtcatgcc ctgtcctctg ctggttggtg gtagaactct gcccatattg
     3121 gaagggaagc ctccagaaag gccttcaaac agatgcagag atccaccaag agaagaagaa
     3181 agagagaaaa agaagaagaa gcacaaaaaa cggtctcgga caaggtcccg ctctcccaag
     3241 taccactcgt cttccaagcc caggtctaga tcccactcaa aagcaaagca ctccctgccc
     3301 agtgcctacc ggacagtgcg gcggtcaagg tcacggtctc ggtccccaag aaggagagct
     3361 cactctcctg agagacggcg ggaagaaagg agtgtcccta cagcctaccg gatgagcggc
     3421 agccctgggg tcagcaggaa aaggacccgt tccagaagtc cccacgagaa gaagaagaaa
     3481 cggcgctcac ggtctcgcac caaagccaag gcccggtcgc agtcaacatc cccgagtaaa
     3541 caggccgcac agcgtccctc ggcccactca gcccactcgg ccagcatctc tcctgtggag
     3601 agccgaggtt cctcccagga acgctccagg ggggtttctc aggaaaaaga tggccagatc
     3661 tcctcagcaa tcgtctcctc tgtgcagagc aaaataactc aggatctcat ggccaaagta
     3721 agagcaatgc tcgcagcttc aaaaaacctg caaaccagtg catccggcgg cggagggagt
     3781 catcatcacc atcaccatta gctcgagtct agagggcccg tttaaacccg ctgatcagcc
     3841 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3901 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3961 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4021 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4081 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     4141 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4201 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4261 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4321 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4381 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4441 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4501 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     4561 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     4621 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     4681 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4741 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4801 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     4861 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     4921 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     4981 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     5041 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     5101 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     5161 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     5221 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     5281 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     5341 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     5401 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     5461 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     5521 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     5581 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     5641 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     5701 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     5761 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     5821 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5881 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5941 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6001 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     6061 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6121 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6181 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6241 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6301 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6361 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6421 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6481 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6541 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6601 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6661 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6721 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6781 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6841 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6901 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6961 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7021 tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7081 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7141 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7201 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7261 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7321 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7381 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7441 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     7501 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     7561 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     7621 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     7681 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7741 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7801 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7861 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7921 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7981 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8041 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8101 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8161 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8221 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggt
//
