LOCUS       62056_6020        9506 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             944..1009
                     /codon_start=1
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1016..1036
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             1061..3718
                     /codon_start=1
                     /label=NgAgo
                     /note="Argonaute protein from Natronobacterium gregoryi
                     (Gao et al., 2016)"
                     /translation="TVIDLDSTTTADELTSGHTYDISVTLTGVYDNTDEQHPRMSLAFE
                     QDNGERRYITLWKNTTPKDVFTYDYATGSTYIFTNIDYEVKDGYENLTATYQTTVENAT
                     AQEVGTTDEDETFAGGEPLDHHLDDALNETPDDAETESDSGHVMTSFASRDQLPEWTLH
                     TYTLTATDGAKTDTEYARRTLAYTVRQELYTDHDAAPVATDGLMLLTPEPLGETPLDLD
                     CGVRVEADETRTLDYTTAKDRLLARELVEEGLKRSLWDDYLVRGIDEVLSKEPVLTCDE
                     FDLHERYDLSVEVGHSGRAYLHINFRHRFVPKLTLADIDDDNIYPGLRVKTTYRPRRGH
                     IVWGLRDECATDSLNTLGNQSVVAYHRNNQTPINTDLLDAIEAADRRVVETRRQGHGDD
                     AVSFPQELLAVEPNTHQIKQFASDGFHQQARSKTRLSASRCSEKAQAFAERLDPVRLNG
                     STVEFSSEFFTGNNEQQLRLLYENGESVLTFRDGARGAHPDETFSKGIVNPPESFEVAV
                     VLPEQQADTCKAQWDTMADLLNQAGAPPTRSETVQYDAFSSPESISLNVAGAIDPSEVD
                     AAFVVLPPDQEGFADLASPTETYDELKKALANMGIYSQMAYFDRFRDAKIFYTRNVALG
                     LLAAAGGVAFTTEHAMPGDADMFIGIDVSRSYPEDGASGQINIAATATAVYKDGTILGH
                     SSTRPQLGEKLQSTDVRDIMKNAILGYQQVTGESPTHIVIHRDGFMNEDLDPATEFLNE
                     QGVEYDIVEIRKQPQTRLLAVSDVQYDTPVKSIAAINQNEPRATVATFGAPEYLATRDG
                     GGLPRPIQIERVAGETDIETLTRQVYLLSQSHIQVHNSTARLPITTAYADQASTHATKG
                     YLVQTGAFESNVGFL"
     CDS             3719..3766
                     /codon_start=1
                     /label=nucleoplasmin NLS
                     /note="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    3793..4372
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             4367..5059
                     /codon_start=1
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     polyA_signal    5106..5330
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5376..5804
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5818..6147
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6214..7005
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    7182..7315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7352..7368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7376..7392)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7400..7430)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7445..7466)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7754..8339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8513..9370)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9371..9475)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atggactata aggaccacga
      961 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
     1021 gaagaagcgg aaggtcggta tccacggagt cccagcagcc accgtgatcg acctggacag
     1081 caccaccacc gccgacgagc tgaccagcgg ccacacctac gacatcagcg tgaccctgac
     1141 cggcgtgtac gacaacaccg acgagcagca cccccgcatg agcctggcct tcgagcagga
     1201 caacggcgag cgccgctaca tcaccctgtg gaagaacacc acccccaagg acgtgttcac
     1261 ctacgactac gccaccggca gcacctacat cttcaccaac atcgactacg aggtgaagga
     1321 cggctacgag aacctgaccg ccacctacca gaccaccgtg gagaacgcca ccgcccagga
     1381 ggtgggcacc accgacgagg acgagacctt cgccggcggc gagcccctgg accaccacct
     1441 ggacgacgcc ctgaacgaga cccccgacga cgccgagacc gagagcgaca gcggccacgt
     1501 gatgaccagc ttcgccagcc gcgaccagct gcccgagtgg accctgcaca cctacaccct
     1561 gaccgccacc gacggcgcca agaccgacac cgagtacgcc cgccgcaccc tggcctacac
     1621 cgtgcgccag gagctgtaca ccgaccacga cgccgccccc gtggccaccg acggcctgat
     1681 gctgctgacc cccgagcccc tgggcgagac ccccctggac ctggactgcg gcgtgcgcgt
     1741 ggaggccgac gagacccgca ccctggacta caccaccgcc aaggaccgcc tgctggcccg
     1801 cgagctggtg gaggagggcc tgaagcgcag cctgtgggac gactacctgg tgcgcggcat
     1861 cgacgaggtg ctgagcaagg agcccgtgct gacctgcgac gagttcgacc tgcacgagcg
     1921 ctacgacctg agcgtggagg tgggccacag cggccgcgcc tacctgcaca tcaacttccg
     1981 ccaccgcttc gtgcccaagc tgaccctggc cgacatcgac gacgacaaca tctaccccgg
     2041 cctgcgcgtg aagaccacct accgcccccg ccgcggccac atcgtgtggg gcctgcgcga
     2101 cgagtgcgcc accgacagcc tgaacaccct gggcaaccag agcgtggtgg cctaccaccg
     2161 caacaaccag acccccatca acaccgacct gctggacgcc atcgaggccg ccgaccgccg
     2221 cgtggtggag acccgccgcc agggccacgg cgacgacgcc gtgagcttcc cccaggagct
     2281 gctggccgtg gagcccaaca cccaccagat caagcagttc gccagcgacg gcttccacca
     2341 gcaggcccgc agcaagaccc gcctgagcgc cagccgctgc agcgagaagg cccaggcctt
     2401 cgccgagcgc ctggaccccg tgcgcctgaa cggcagcacc gtggagttca gcagcgagtt
     2461 cttcaccggc aacaacgagc agcagctgcg cctgctgtac gagaacggcg agagcgtgct
     2521 gaccttccgc gacggcgccc gcggcgccca ccccgacgag accttcagca agggcatcgt
     2581 gaaccccccc gagagcttcg aggtggccgt ggtgctgccc gagcagcagg ccgacacctg
     2641 caaggcccag tgggacacca tggccgacct gctgaaccag gccggcgccc cccccacccg
     2701 cagcgagacc gtgcagtacg acgccttcag cagccccgag agcatcagcc tgaacgtggc
     2761 cggcgccatc gaccccagcg aggtggacgc cgccttcgtg gtgctgcccc ccgaccagga
     2821 gggcttcgcc gacctggcca gccccaccga gacctacgac gagctgaaga aggccctggc
     2881 caacatgggc atctacagcc agatggccta cttcgaccgc ttccgcgacg ccaagatctt
     2941 ctacacccgc aacgtggccc tgggcctgct ggccgccgcc ggcggcgtgg ccttcaccac
     3001 cgagcacgcc atgcccggcg acgccgacat gttcatcggc atcgacgtga gccgcagcta
     3061 ccccgaggac ggcgccagcg gccagatcaa catcgccgcc accgccaccg ccgtgtacaa
     3121 ggacggcacc atcctgggcc acagcagcac ccgcccccag ctgggcgaga agctgcagag
     3181 caccgacgtg cgcgacatca tgaagaacgc catcctgggc taccagcagg tgaccggcga
     3241 gagccccacc cacatcgtga tccaccgcga cggcttcatg aacgaggacc tggaccccgc
     3301 caccgagttc ctgaacgagc agggcgtgga gtacgacatc gtggagatcc gcaagcagcc
     3361 ccagacccgc ctgctggccg tgagcgacgt gcagtacgac acccccgtga agagcatcgc
     3421 cgccatcaac cagaacgagc cccgcgccac cgtggccacc ttcggcgccc ccgagtacct
     3481 ggccacccgc gacggcggcg gcctgccccg ccccatccag atcgagcgcg tggccggcga
     3541 gaccgacatc gagaccctga cccgccaggt gtacctgctg agccagagcc acatccaggt
     3601 gcacaacagc accgcccgcc tgcccatcac caccgcctac gccgaccagg ccagcaccca
     3661 cgccaccaag ggctacctgg tgcagaccgg cgccttcgag agcaacgtgg gcttcctgaa
     3721 aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag aaaaagtgag aattctgcag
     3781 atatcggaat tccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     3841 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     3901 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     3961 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     4021 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     4081 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     4141 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tcacctcaag
     4201 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct
     4261 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc
     4321 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg cccagtccaa
     4381 gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg tggacggcca
     4441 caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc aggccatcaa
     4501 cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt ccgccgcctt
     4561 catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact acttcaagaa
     4621 ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg
     4681 catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa
     4741 gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga ccgacaactg
     4801 ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga agggcgacgt
     4861 gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta
     4921 caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc acaagctgac
     4981 ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc acgccatcgc
     5041 ctccggctcc gccttgccct gactcgagtc tagagggccc gtttaaaccc gctgatcagc
     5101 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5161 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     5221 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     5281 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5341 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     5401 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5461 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5521 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5581 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5641 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5701 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5761 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     5821 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5881 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     5941 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6001 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6061 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     6121 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     6181 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     6241 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6301 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6361 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     6421 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6481 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6541 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6601 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6661 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6721 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     6781 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6841 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6901 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6961 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     7021 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     7081 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7141 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     7201 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7261 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7321 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7381 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7441 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7501 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7561 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7621 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7681 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7741 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7801 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7861 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7921 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7981 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8041 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8101 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8161 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8221 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8281 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8341 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8401 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8461 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8521 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8581 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8641 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8701 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8761 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8821 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8881 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8941 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9001 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9061 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9121 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9181 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9241 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9301 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9361 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9421 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9481 ttccccgaaa agtgccacct gacgtc
//
