LOCUS       62056_6015        9803 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             303..323
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             324..344
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             345..365
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             387..1103
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1143..1169
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             1173..1199
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             1257..4649
                     /codon_start=1
                     /label=hTERT
                     /note="human telomerase reverse transcriptase, the
                     catalytic subunit of telomerase"
                     /translation="PRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAA
                     FRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALLD
                     GARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVAP
                     SCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARR
                     RGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEA
                     TSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQL
                     RPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNH
                     AQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPW
                     QVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCA
                     WLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFYR
                     KSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNM
                     DYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVL
                     RVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGH
                     VRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRFM
                     CHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLL
                     VTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWC
                     GLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDLQ
                     VNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILKA
                     KNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLP
                     GTTLTALEAAANPALPSDFKTILD"
     CDS             4693..4722
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             4738..4755
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    4784..5008
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5054..5482
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5496..5825
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5892..6683
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6862..6995
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7032..7048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7056..7072)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7080..7110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7125..7146)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7434..8022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8196..9053)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9054..9158)
                     /label=AmpR promoter
     enhancer        9424..9803
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag cttgccacca
      301 tgccaaaaaa gaagagaaag gtaccaaaaa agaagagaaa ggtaccaaaa aagaagagaa
      361 aggtacggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      421 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      481 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      541 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      601 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      661 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      721 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      781 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      841 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      901 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      961 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1021 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1081 tcggcatgga cgagctgtac aagcgaattc tgcagataaa cgccaccatg gggcaacaag
     1141 gatacccgta tgatgttccg gattacgctg gctacccata cgacgtccca gactacgctg
     1201 gctacccata cgacgtccca gactacgctc aaggagtgcg gccgcggggg cgcgccccgc
     1261 gcgctccccg ctgccgagcc gtgcgctccc tgctgcgcag ccactaccgc gaggtgctgc
     1321 cgctggccac gttcgtgcgg cgcctggggc cccagggctg gcggctggtg cagcgcgggg
     1381 acccggcggc tttccgcgcg ctggtggccc agtgcctggt gtgcgtgccc tgggacgcac
     1441 ggccgccccc cgccgccccc tccttccgcc aggtgtcctg cctgaaggag ctggtggccc
     1501 gagtgctgca gaggctgtgc gagcgcggcg cgaagaacgt gctggccttc ggcttcgcgc
     1561 tgctggacgg ggcccgcggg ggcccccccg aggccttcac caccagcgtg cgcagctacc
     1621 tgcccaacac ggtgaccgac gcactgcggg ggagcggggc gtgggggctg ctgctgcgcc
     1681 gcgtgggcga cgacgtgctg gttcacctgc tggcacgctg cgcgctcttt gtgctggtgg
     1741 ctcccagctg cgcctaccag gtgtgcgggc cgccgctgta ccagctcggc gctgccactc
     1801 aggcccggcc cccgccacac gctagtggac cccgaaggcg tctgggatgc gaacgggcct
     1861 ggaaccatag cgtcagggag gccggggtcc ccctgggcct gccagccccg ggtgcgagga
     1921 ggcgcggggg cagtgccagc cgaagtctgc cgttgcccaa gaggcccagg cgtggcgctg
     1981 cccctgagcc ggagcggacg cccgttgggc aggggtcctg ggcccacccg ggcaggacgc
     2041 gtggaccgag tgaccgtggt ttctgtgtgg tgtcacctgc cagacccgcc gaagaagcca
     2101 cctctttgga gggtgcgctc tctggcacgc gccactccca cccatccgtg ggccgccagc
     2161 accacgcggg ccccccatcc acatcgcggc caccacgtcc ctgggacacg ccttgtcccc
     2221 cggtgtacgc cgagaccaag cacttcctct actcctcagg cgacaaggag cagctgcggc
     2281 cctccttcct actcagctct ctgaggccca gcctgactgg cgctcggagg ctcgtggaga
     2341 ccatctttct gggttccagg ccctggatgc cagggactcc ccgcaggttg ccccgcctgc
     2401 cccagcgcta ctggcaaatg cggcccctgt ttctggagct gcttgggaac cacgcgcagt
     2461 gcccctacgg ggtgctcctc aagacgcact gcccgctgcg agctgcggtc accccagcag
     2521 ccggtgtctg tgcccgggag aagccccagg gctctgtggc ggcccccgag gaggaggaca
     2581 cagacccccg tcgcctggtg cagctgctcc gccagcacag cagcccctgg caggtgtacg
     2641 gcttcgtgcg ggcctgcctg cgccggctgg tgcccccagg cctctggggc tccaggcaca
     2701 acgaacgccg cttcctcagg aacaccaaga agttcatctc cctggggaag catgccaagc
     2761 tctcgctgca ggagctgacg tggaagatga gcgtgcggga ctgcgcttgg ctgcgcagga
     2821 gcccaggggt tggctgtgtt ccggccgcag agcaccgtct gcgtgaggag atcctggcca
     2881 agttcctgca ctggctgatg agtgtgtacg tcgtcgagct gctcaggtct ttcttttatg
     2941 tcacggagac cacgtttcaa aagaacaggc tctttttcta ccggaagagt gtctggagca
     3001 agttgcaaag cattggaatc agacagcact tgaagagggt gcagctgcgg gagctgtcgg
     3061 aagcagaggt caggcagcat cgggaagcca ggcccgccct gctgacgtcc agactccgct
     3121 tcatccccaa gcctgacggg ctgcggccga ttgtgaacat ggactacgtc gtgggagcca
     3181 gaacgttccg cagagaaaag agggccgagc gtctcacctc gagggtgaag gcactgttca
     3241 gcgtgctcaa ctacgagcgg gcgcggcgcc ccggcctcct gggcgcctct gtgctgggcc
     3301 tggacgatat ccacagggcc tggcgcacct tcgtgctgcg tgtgcgggcc caggacccgc
     3361 cgcctgagct gtactttgtc aaggtggatg tgacgggcgc gtacgacacc atcccccagg
     3421 acaggctcac ggaggtcatc gccagcatca tcaaacccca gaacacgtac tgcgtgcgtc
     3481 ggtatgccgt ggtccagaag gccgcccatg ggcacgtccg caaggccttc aagagccacg
     3541 tctctacctt gacagacctc cagccgtaca tgcgacagtt cgtggctcac ctgcaggaga
     3601 ccagcccgct gagggatgcc gtcgtcatcg agcagagctc ctccctgaat gaggccagca
     3661 gtggcctctt cgacgtcttc ctacgcttca tgtgccacca cgccgtgcgc atcaggggca
     3721 agtcctacgt ccagtgccag gggatcccgc agggctccat cctctccacg ctgctctgca
     3781 gcctgtgcta cggcgacatg gagaacaagc tgtttgcggg gattcggcgg gacgggctgc
     3841 tcctgcgttt ggtggatgat ttcttgttgg tgacacctca cctcacccac gcgaaaacct
     3901 tcctcaggac cctggtccga ggtgtccctg agtatggctg cgtggtgaac ttgcggaaga
     3961 cagtggtgaa cttccctgta gaagacgagg ccctgggtgg cacggctttt gttcagatgc
     4021 cggcccacgg cctattcccc tggtgcggcc tgctgctgga tacccggacc ctggaggtgc
     4081 agagcgacta ctccagctat gcccggacct ccatcagagc cagtctcacc ttcaaccgcg
     4141 gcttcaaggc tgggaggaac atgcgtcgca aactctttgg ggtcttgcgg ctgaagtgtc
     4201 acagcctgtt tctggatttg caggtgaaca gcctccagac ggtgtgcacc aacatctaca
     4261 agatcctcct gctgcaggcg tacaggtttc acgcatgtgt gctgcagctc ccatttcatc
     4321 agcaagtttg gaagaacccc acatttttcc tgcgcgtcat ctctgacacg gcctccctct
     4381 gctactccat cctgaaagcc aagaacgcag ggatgtcgct gggggccaag ggcgccgccg
     4441 gccctctgcc ctccgaggcc gtgcagtggc tgtgccacca agcattcctg ctcaagctga
     4501 ctcgacaccg tgtcacctac gtgccactcc tggggtcact caggacagcc cagacgcagc
     4561 tgagtcggaa gctcccgggg acgacgctga ctgccctgga ggccgcagcc aacccggcac
     4621 tgccctcaga cttcaagacc atcctggact gattaattaa gtctagagtc gagtctagag
     4681 ggcccgcggt tcgaacaaaa actcatctca gaagaggatc tgaatatgca taccggtcat
     4741 catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     4801 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     4861 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     4921 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     4981 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     5041 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5101 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5161 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5221 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5281 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5341 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5401 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5461 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     5521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5581 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5641 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     5701 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     5761 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     5821 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     5881 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5941 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     6001 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     6061 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     6121 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     6181 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     6241 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     6301 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     6361 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     6421 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     6481 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6541 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6601 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6661 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa
     6721 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6781 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6841 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6901 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6961 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     7021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7261 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7321 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7861 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7981 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     8041 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     8101 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8161 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8221 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8281 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8341 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8401 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8461 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8521 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8581 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8641 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8701 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8761 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8821 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8881 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8941 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     9001 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     9061 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9121 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9181 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     9241 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     9301 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     9361 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     9421 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     9481 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     9541 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     9601 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     9661 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     9721 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     9781 tattagtcat cgctattacc atg
//
