LOCUS       V013423                 7685 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013423
VERSION     V013423
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             896..3166
                     /gene="PB1"
                     /label="RNA-directed RNA polymerase catalytic subunit"
                     /note="RNA-directed RNA polymerase catalytic subunit from
                     Influenza A virus (strain A/Puerto Rico/8/1934 H1N1).
                     Accession#: P03431"
     CDS             3182..3205
                     /label="8xHis"
                     /note="8xHis affinity tag"
     polyA_signal    3252..3476
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3522..3950
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3964..4293
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4360..5151
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5328..5461
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5498..5514)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5522..5538)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5546..5576)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5591..5612)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5900..6485)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6659..7516)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7517..7621)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatgga
      901 tgtcaatccg accttacttt tcttaaaagt gccagcacaa aatgctataa gcacaacttt
      961 cccttatacc ggagaccctc cttacagcca tgggacagga acaggataca ccatggatac
     1021 tgtcaacagg acacatcagt actcagaaaa ggcaagatgg acaacaaaca ccgaaactgg
     1081 agcaccgcaa ctcaacccga ttgatgggcc actgccagaa gacaatgaac caagtggtta
     1141 tgcccaaaca gattgtgtat tggaagcaat ggctttcctt gaggaatccc atcctggtat
     1201 ttttgaaaac tcgtgtattg aaacgatgga ggttgttcag caaacacgag tagacaagct
     1261 gacacaaggc cgacagacct atgactggac tttaaataga aaccagcctg ctgcaacagc
     1321 attggccaac acaatagaag tgttcagatc aaatggcctc acggccaatg agtctggaag
     1381 gctcatagac ttccttaagg atgtaatgga gtcaatgaaa aaagaagaaa tggggatcac
     1441 aactcatttt cagagaaaga gacgggtgag agacaatatg actaagaaaa tgataacaca
     1501 gagaacaata ggtaaaagga aacagagatt gaacaaaagg agttatctaa ttagagcatt
     1561 gaccctgaac acaatgacca aagatgctga gagagggaag ctaaaacgga gagcaattgc
     1621 aaccccaggg atgcaaataa gggggtttgt atactttgtt gagacactgg caaggagtat
     1681 atgtgagaaa cttgaacaat cagggttgcc agttggaggc aatgagaaga aagcaaagtt
     1741 ggcaaatgtt gtaaggaaga tgatgaccaa ttctcaggac accgaacttt ctttgaccat
     1801 cactggagat aacaccaaat ggaacgaaaa tcagaatcct cggatgtttt tggccatgat
     1861 cacatatatg accagaaatc agcccgaatg gttcagaaat gttctaagta ttgctccaat
     1921 aatgttctca aacaaaatgg cgagactggg aaaagggtat atgtttgaga gcaagagtat
     1981 gaaacttaga actcaaatac ctgcagaaat gctagcaagc attgatttga aatatttcaa
     2041 tgattcaaca agaaagaaga ttgaaaaaat ccgaccgctc ttaatagagg ggactgcatc
     2101 attgagccct ggaatgatga tgggcatgtt caatatgtta agcactgtat taggcgtctc
     2161 catcctgaat cttggacaaa agagatacac caagactact tactggtggg atggtcttca
     2221 atcctctgac gattttgctc tgattgtgaa tgcacccaat catgaaggga ttcaagccgg
     2281 agtcgacagg ttttatcgaa cctgtaagct acatggaatc aatatgagca agaaaaagtc
     2341 ttacataaac agaacaggta catttgaatt cacaagtttt ttctatcgtt atgggtttgt
     2401 tgccaatttc agcatggagc ttcccagttt tggtgtgtct gggagcaacg agtcagcgga
     2461 catgagtatt ggagttactg tcatcaaaaa caatatgata aacaatgatc ttggtccagc
     2521 aacagctcaa atggcccttc agttgttcat caaagattac aggtacacgt accgatgcca
     2581 tagaggtgac acacaaatac aaacccgaag atcatttgaa ataaagaaac tgtgggagca
     2641 aacccgttcc aaagctggac tgctggtctc cgacggaggc ccaaatttat acaacattag
     2701 aaatctccac attcctgaag tctgcctaaa atgggaattg atggatgagg attaccaggg
     2761 gcgtttatgc aacccactga acccatttgt cagccataaa gaaattgaat caatgaacaa
     2821 tgcagtgatg atgccagcac atggtccagc caaaaacatg gagtatgatg ctgttgcaac
     2881 aacacactcc tggatcccca aaagaaatcg atccatcttg aatacaagtc aaagaggagt
     2941 acttgaagat gaacaaatgt accaaaggtg ctgcaattta tttgaaaaat tcttccccag
     3001 cagttcatac agaagaccag tcgggatatc cagtatggtg gaggctatgg tttccagagc
     3061 ccgaattgat gcacggattg atttcgaatc tggaaggata aagaaagaag agttcactga
     3121 gatcatgaag atctgttcca ccattgaaga gctcagacgg caaaaaggag gcggcggttc
     3181 ccatcaccac catcaccacc atcattgact cgagtctaga gggcccgttt aaacccgctg
     3241 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     3301 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     3361 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     3421 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     3481 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     3541 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3601 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3661 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3721 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3781 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3841 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     3961 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4021 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4081 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4201 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4261 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     4321 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     4381 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4441 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4501 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     4561 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     4621 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     4681 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     4741 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     4801 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     4861 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     4921 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     4981 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5041 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5101 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     5161 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     5221 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     5281 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     5341 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5401 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     5461 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5521 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5581 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5641 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5701 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     5761 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     5821 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     5881 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5941 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6001 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6061 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6121 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6181 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6241 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6301 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6361 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6421 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6481 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6541 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6601 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6781 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6961 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7021 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7081 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7141 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7201 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7261 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7321 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7381 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7441 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7501 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7561 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7621 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     7681 atggt
//
