LOCUS       V015170                 8233 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015170
VERSION     V015170
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             931..3753
                     /gene="GABBR2"
                     /label="Gamma-aminobutyric acid type B receptor subunit 2"
                     /note="Gamma-aminobutyric acid type B receptor subunit 2
                     from Homo sapiens. Accession#: O75899"
     polyA_signal    3800..4024
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4070..4498
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4512..4841
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4908..5699
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5876..6009
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6046..6062)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6070..6086)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6094..6124)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6139..6160)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6448..7033)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7207..8064)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8065..8169)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcttccc cgcggagctc cgggcagccc
      961 gggccgccgc cgccgccgcc accgccgccc gcgcgcctgc tactgctact gctgctgccg
     1021 ctgctgctgc ctctggcgcc cggggcctgg ggctgggcgc ggggcgcccc ccggccgccg
     1081 cccagcagcc cgccgctctc catcatgggc ctcatgccgc tcaccaagga ggtggccaag
     1141 ggcagcatcg ggcgcggtgt gctccccgcc gtggaactgg ccatcgagca gatccgcaac
     1201 gagtcactcc tgcgccccta cttcctcgac ctgcggctct atgacacgga gtgcgacaac
     1261 gcaaaagggt tgaaagcctt ctacgatgca ataaaatacg ggcctaacca cttgatggtg
     1321 tttggaggcg tctgtccatc cgtcacatcc atcattgcag agtccctcca aggctggaat
     1381 ctggtgcagc tttcttttgc tgcaaccacg cctgttctag ccgataagaa aaaataccct
     1441 tatttctttc ggaccgtccc atcagacaat gcggtgaatc cagccattct gaagttgctc
     1501 aagcactacc agtggaagcg cgtgggcacg ctgacgcaag acgttcagag gttctctgag
     1561 gtgcggaatg acctgactgg agttctgtat ggcgaggaca ttgagatttc agacaccgag
     1621 agcttctcca acgatccctg taccagtgtc aaaaagctga aggggaatga tgtgcggatc
     1681 atccttggcc agtttgacca gaatatggca gcaaaagtgt tctgttgtgc atacgaggag
     1741 aacatgtatg gtagtaaata tcagtggatc attccgggct ggtacgagcc ttcttggtgg
     1801 gagcaggtgc acacggaagc caactcatcc cgctgcctcc ggaagaatct gcttgctgcc
     1861 atggagggct acattggcgt ggatttcgag cccctgagct ccaagcagat caagaccatc
     1921 tcaggaaaga ctccacagca gtatgagaga gagtacaaca acaagcggtc aggcgtgggg
     1981 cccagcaagt tccacgggta cgcctacgat ggcatctggg tcatcgccaa gacactgcag
     2041 agggccatgg agacactgca tgccagcagc cggcaccagc ggatccagga cttcaactac
     2101 acggaccaca cgctgggcag gatcatcctc aatgccatga acgagaccaa cttcttcggg
     2161 gtcacgggtc aagttgtatt ccggaatggg gagagaatgg ggaccattaa atttactcaa
     2221 tttcaagaca gcagggaggt gaaggtggga gagtacaacg ctgtggccga cacactggag
     2281 atcatcaatg acaccatcag gttccaagga tccgaaccac caaaagacaa gaccatcatc
     2341 ctggagcagc tgcggaagat ctccctacct ctctacagca tcctctctgc cctcaccatc
     2401 ctcgggatga tcatggccag tgcttttctc ttcttcaaca tcaagaaccg gaatcagaag
     2461 ctcataaaga tgtcgagtcc atacatgaac aaccttatca tccttggagg gatgctctcc
     2521 tatgcttcca tatttctctt tggccttgat ggatcctttg tctctgaaaa gacctttgaa
     2581 acactttgca ccgtcaggac ctggattctc accgtgggct acacgaccgc ttttggggcc
     2641 atgtttgcaa agacctggag agtccacgcc atcttcaaaa atgtgaaaat gaagaagaag
     2701 atcatcaagg accagaaact gcttgtgatc gtggggggca tgctgctgat cgacctgtgt
     2761 atcctgatct gctggcaggc tgtggacccc ctgcgaagga cagtggagaa gtacagcatg
     2821 gagccggacc cagcaggacg ggatatctcc atccgccctc tcctggagca ctgtgagaac
     2881 acccatatga ccatctggct tggcatcgtc tatgcctaca agggacttct catgttgttc
     2941 ggttgtttct tagcttggga gacccgcaac gtcagcatcc ccgcactcaa cgacagcaag
     3001 tacatcggga tgagtgtcta caacgtgggg atcatgtgca tcatcggggc cgctgtctcc
     3061 ttcctgaccc gggaccagcc caatgtgcag ttctgcatcg tggctctggt catcatcttc
     3121 tgcagcacca tcaccctctg cctggtattc gtgccgaagc tcatcaccct gagaacaaac
     3181 ccagatgcag caacgcagaa caggcgattc cagttcactc agaatcagaa gaaagaagat
     3241 tctaaaacgt ccacctcggt caccagtgtg aaccaagcca gcacatcccg cctggagggc
     3301 ctacagtcag aaaaccatcg cctgcgaatg aagatcacag agctggataa agacttggaa
     3361 gaggtcacca tgcagctgca ggacacacca gaaaagacca cctacattaa acagaaccac
     3421 taccaagagc tcaatgacat cctcaacctg ggaaacttca ctgagagcac agatggagga
     3481 aaggccattt taaaaaatca cctcgatcaa aatccccagc tacagtggaa cacaacagag
     3541 ccctctcgaa catgcaaaga tcctatagaa gatataaact ctccagaaca catccagcgt
     3601 cggctgtccc tccagctccc catcctccac cacgcctacc tcccatccat cggaggcgtg
     3661 gacgccagct gtgtcagccc ctgcgtcagc cccaccgcca gcccccgcca cagacatgtg
     3721 ccaccctcct tccgagtcat ggtctcgggc ctgtaactcg agtctagagg gcccgtttaa
     3781 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3841 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3901 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3961 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     4021 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4501 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4561 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4621 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4681 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4741 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4801 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4861 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4921 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4981 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     5041 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     5101 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     5161 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     5221 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     5281 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     5341 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     5401 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5461 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5521 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5581 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5641 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5701 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     5761 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     5821 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     5881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6001 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6061 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6961 caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7021 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7081 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7141 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7201 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7261 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7321 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7381 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7441 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7501 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7561 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7621 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7681 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7741 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7801 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7861 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7921 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7981 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8041 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8101 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8161 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
     8221 gatcccctat ggt
//
