LOCUS       62056_5830        9079 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9079
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3070..3321
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     polyA_signal    4646..4870
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4916..5344
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5358..5687
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5754..6545
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6722..6855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6892..6908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6916..6932)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6940..6970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6985..7006)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7294..7879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8053..8910)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8911..9015)
                     /label=AmpR promoter
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcttccc cgcggagctc cgggcagccc
      961 gggccgccgc cgccgccgcc accgccgccc gcgcgcctgc tactgctact gctgctgccg
     1021 ctgctgctgc ctctggcgcc cggggcctgg ggctgggcgc ggggcgcccc ccggccgccg
     1081 cccagcagcc cgccgctctc catcatgggc ctcatgccgc tcaccaagga ggtggccaag
     1141 ggcagcatcg ggcgcggtgt gctccccgcc gtggaactgg ccatcgagca gatccgcaac
     1201 gagtcactcc tgcgccccta cttcctcgac ctgcggctct atgacacgga gtgcgacaac
     1261 gcaaaagggt tgaaagcctt ctacgatgca ataaaatacg ggcctaacca cttgatggtg
     1321 tttggaggcg tctgtccatc cgtcacatcc atcattgcag agtccctcca aggctggaat
     1381 ctggtgcagc tttcttttgc tgcaaccacg cctgttctag ccgataagaa aaaataccct
     1441 tatttctttc ggaccgtccc atcagacaat gcggtgaatc cagccattct gaagttgctc
     1501 aagcactacc agtggaagcg cgtgggcacg ctgacgcaag acgttcagag gttctctgag
     1561 gtgcggaatg acctgactgg agttctgtat ggcgaggaca ttgagatttc agacaccgag
     1621 agcttctcca acgatccctg taccagtgtc aaaaagctga aggggaatga tgtgcggatc
     1681 atccttggcc agtttgacca gaatatggca gcaaaagtgt tctgttgtgc atacgaggag
     1741 aacatgtatg gtagtaaata tcagtggatc attccgggct ggtacgagcc ttcttggtgg
     1801 gagcaggtgc acacggaagc caactcatcc cgctgcctcc ggaagaatct gcttgctgcc
     1861 atggagggct acattggcgt ggatttcgag cccctgagct ccaagcagat caagaccatc
     1921 tcaggaaaga ctccacagca gtatgagaga gagtacaaca acaagcggtc aggcgtgggg
     1981 cccagcaagt tccacgggta cgcctacgat ggcatctggg tcatcgccaa gacactgcag
     2041 agggccatgg agacactgca tgccagcagc cggcaccagc ggatccagga cttcaactac
     2101 acggaccaca cgctgggcag gatcatcctc aatgccatga acgagaccaa cttcttcggg
     2161 gtcacgggtc aagttgtatt ccggaatggg gagagaatgg ggaccattaa atttactcaa
     2221 tttcaagaca gcagggaggt gaaggtggga gagtacaacg ctgtggccga cacactggag
     2281 atcatcaatg acaccatcag gttccaagga tccgaaccac caaaagacaa gaccatcatc
     2341 ctggagcagc tgcggaagat ctccctacct ctctacagca tcctctctgc cctcaccatc
     2401 ctcgggatga tcatggccag tgcttttctc ttcttcaaca tcaagaaccg gaatcagaag
     2461 ctcataaaga tgtcgagtcc atacatgaac aaccttatca tccttggagg gatgctctcc
     2521 tatgcttcca tatttctctt tggccttgat ggatcctttg tctctgaaaa gacctttgaa
     2581 acactttgca ccgtcaggac ctggattctc accgtgggct acacgaccgc ttttggggcc
     2641 atgtttgcaa agacctggag agtccacgcc atcttcaaaa atgtgaaaat gaagaagaag
     2701 atcatcaagg accagaaact gcttgtgatc gtggggggca tgctgctgat cgacctgtgt
     2761 atcctgatct gctggcaggc tgtggacccc ctgcgaagga cagtggagaa gtacagcatg
     2821 gagccggacc cagcaggacg ggatatctcc atccgccctc tcctggagca ctgtgagaac
     2881 acccatatga ccatctggct tggcatcgtc tatgcctaca agggacttct catgttgttc
     2941 ggttgtttct tagcttgggt ctttcaggag gccaaaaggc agctccagaa gattgacaaa
     3001 tctgagggcc gcttccatgt ccagaacctt agccaggtgg agcagggagg aaacgtctat
     3061 atcaaggccg acaagcagaa gaacggcatc aaggcgaact tccacatccg ccacaacatc
     3121 gaggacggcg gcgtgcagct cgcctaccac taccagcaga acacccccat cggcgacggc
     3181 cccgtgctgc tgcccgacaa ccactacctg agcgtgcagt ccaaactttc gaaagacccc
     3241 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3301 ggcatggacg agctgtacaa gggcggtacc ggagggagca tggtgagaaa gggcgaggag
     3361 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3421 ttcagcgtgt ccggcgaggg tgagggcgat gccacctacg gcaagctgac cctgaagttc
     3481 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3541 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3601 gccatgcccg aaggctacat ccaggagcgc accatcttct tcaaggacga cggcaactac
     3661 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3721 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caacgctcct
     3781 agcgtcgcag atgggcggac ggggcatgga ctccgcagat cttccaagtt ctgcttgaag
     3841 gagcacaaag ccctcaagac ggtctacaac gtggggatca tgtgcatcat cggggccgct
     3901 gtctccttcc tgacccggga ccagcccaat gtgcagttct gcatcgtggc tctggtcatc
     3961 atcttctgca gcaccatcac cctctgcctg gtattcgtgc cgaagctcat caccctgaga
     4021 acaaacccag atgcagcaac gcagaacagg cgattccagt tcactcagaa tcagaagaaa
     4081 gaagattcta aaacgtccac ctcggtcacc agtgtgaacc aagccagcac atcccgcctg
     4141 gagggcctac agtcagaaaa ccatcgcctg cgaatgaaga tcacagagct ggataaagac
     4201 ttggaagagg tcaccatgca gctgcaggac acaccagaaa agaccaccta cattaaacag
     4261 aaccactacc aagagctcaa tgacatcctc aacctgggaa acttcactga gagcacagat
     4321 ggaggaaagg ccattttaaa aaatcacctc gatcaaaatc cccagctaca gtggaacaca
     4381 acagagccct ctcgaacatg caaagatcct atagaagata taaactctcc agaacacatc
     4441 cagcgtcggc tgtccctcca gctccccatc ctccaccacg cctacctccc atccatcgga
     4501 ggcgtggacg ccagctgtgt cagcccctgc gtcagcccca ccgccagccc ccgccacaga
     4561 catgtgccac cctccttccg agtcatggtc tcgggcctgt aactcgagtc tagagggccc
     4621 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     4681 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     4741 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     4801 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     4861 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     4921 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4981 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     5041 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     5101 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     5161 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     5221 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     5281 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5341 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     5401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5461 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5521 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5581 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     5641 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     5701 cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg
     5761 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     5821 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     5881 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     5941 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     6001 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     6061 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     6121 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     6181 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     6241 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
     6301 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     6361 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     6421 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     6481 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     6541 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     6601 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     6661 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6721 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6781 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6841 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     6901 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     6961 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     7021 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     7081 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     7141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     7201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7801 atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc agattacgcg
     7861 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7921 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7981 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8041 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8101 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8161 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8221 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8281 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8341 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8401 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8461 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8521 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8581 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8641 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8701 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8761 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8821 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8881 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8941 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9001 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     9061 tctcccgatc ccctatggt
//
