LOCUS       62056_5820        8992 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3028..3279
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNH
                     YLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     polyA_signal    4559..4783
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4829..5257
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5271..5600
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5667..6458
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    6635..6768
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6805..6821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6829..6845)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6853..6883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6898..6919)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7207..7792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7966..8823)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8824..8928)
                     /label=AmpR promoter
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atggcttccc cgcggagctc cgggcagccc
      961 gggccgccgc cgccgccgcc accgccgccc gcgcgcctgc tactgctact gctgctgccg
     1021 ctgctgctgc ctctggcgcc cggggcctgg ggctgggcgc ggggcgcccc ccggccgccg
     1081 cccagcagcc cgccgctctc catcatgggc ctcatgccgc tcaccaagga ggtggccaag
     1141 ggcagcatcg ggcgcggtgt gctccccgcc gtggaactgg ccatcgagca gatccgcaac
     1201 gagtcactcc tgcgccccta cttcctcgac ctgcggctct atgacacgga gtgcgacaac
     1261 gcaaaagggt tgaaagcctt ctacgatgca ataaaatacg ggcctaacca cttgatggtg
     1321 tttggaggcg tctgtccatc cgtcacatcc atcattgcag agtccctcca aggctggaat
     1381 ctggtgcagc tttcttttgc tgcaaccacg cctgttctag ccgataagaa aaaataccct
     1441 tatttctttc ggaccgtccc atcagacaat gcggtgaatc cagccattct gaagttgctc
     1501 aagcactacc agtggaagcg cgtgggcacg ctgacgcaag acgttcagag gttctctgag
     1561 gtgcggaatg acctgactgg agttctgtat ggcgaggaca ttgagatttc agacaccgag
     1621 agcttctcca acgatccctg taccagtgtc aaaaagctga aggggaatga tgtgcggatc
     1681 atccttggcc agtttgacca gaatatggca gcaaaagtgt tctgttgtgc atacgaggag
     1741 aacatgtatg gtagtaaata tcagtggatc attccgggct ggtacgagcc ttcttggtgg
     1801 gagcaggtgc acacggaagc caactcatcc cgctgcctcc ggaagaatct gcttgctgcc
     1861 atggagggct acattggcgt ggatttcgag cccctgagct ccaagcagat caagaccatc
     1921 tcaggaaaga ctccacagca gtatgagaga gagtacaaca acaagcggtc aggcgtgggg
     1981 cccagcaagt tccacgggta cgcctacgat ggcatctggg tcatcgccaa gacactgcag
     2041 agggccatgg agacactgca tgccagcagc cggcaccagc ggatccagga cttcaactac
     2101 acggaccaca cgctgggcag gatcatcctc aatgccatga acgagaccaa cttcttcggg
     2161 gtcacgggtc aagttgtatt ccggaatggg gagagaatgg ggaccattaa atttactcaa
     2221 tttcaagaca gcagggaggt gaaggtggga gagtacaacg ctgtggccga cacactggag
     2281 atcatcaatg acaccatcag gttccaagga tccgaaccac caaaagacaa gaccatcatc
     2341 ctggagcagc tgcggaagat ctccctacct ctctacagca tcctctctgc cctcaccatc
     2401 ctcgggatga tcatggccag tgcttttctc ttcttcaaca tcaagaaccg gaatcagaag
     2461 ctcataaaga tgtcgagtcc atacatgaac aaccttatca tccttggagg gatgctctcc
     2521 tatgcttcca tatttctctt tggccttgat ggatcctttg tctctgaaaa gacctttgaa
     2581 acactttgca ccgtcaggac ctggattctc accgtgggct acacgaccgc ttttggggcc
     2641 atgtttgcaa agacctggag agtccacgcc atcttcaaaa atgtgaaaat gaagaagaag
     2701 atcatcaagg accagaaact gcttgtgatc gtggggggca tgctgctgat cgacctgtgt
     2761 atcctgatct gctggcaggc tgtggacccc ctgcgaagga cagtggagaa gtacagcatg
     2821 gagccggacc cagcaggacg ggatatctcc atccgccctc tcctggagca ctgtgagaac
     2881 acccatatga ccatctggct tggcatcgtc tatgcctaca agggacttct catgttgttc
     2941 ggttgtttct tagcttggga gacccgcaac gtcagcatcc ccgcactcaa cggaggtagt
     3001 ggaggcacga acgtctatat caaggccgac aagcagaaga acggcatcaa ggcgaacttc
     3061 cacatccgcc acaacatcga ggacggcggc gtgcagctcg cctaccacta ccagcagaac
     3121 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cgtgcagtcc
     3181 aaactttcga aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3241 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
     3301 gtgagaaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3361 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggtg agggcgatgc cacctacggc
     3421 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3481 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     3541 cacgacttct tcaagtccgc catgcccgaa ggctacatcc aggagcgcac catcttcttc
     3601 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3661 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3721 ctggagtaca acggaggtag tggaggcacg gacagcaagt acatcgggat gagtgtctac
     3781 aacgtgggga tcatgtgcat catcggggcc gctgtctcct tcctgacccg ggaccagccc
     3841 aatgtgcagt tctgcatcgt ggctctggtc atcatcttct gcagcaccat caccctctgc
     3901 ctggtattcg tgccgaagct catcaccctg agaacaaacc cagatgcagc aacgcagaac
     3961 aggcgattcc agttcactca gaatcagaag aaagaagatt ctaaaacgtc cacctcggtc
     4021 accagtgtga accaagccag cacatcccgc ctggagggcc tacagtcaga aaaccatcgc
     4081 ctgcgaatga agatcacaga gctggataaa gacttggaag aggtcaccat gcagctgcag
     4141 gacacaccag aaaagaccac ctacattaaa cagaaccact accaagagct caatgacatc
     4201 ctcaacctgg gaaacttcac tgagagcaca gatggaggaa aggccatttt aaaaaatcac
     4261 ctcgatcaaa atccccagct acagtggaac acaacagagc cctctcgaac atgcaaagat
     4321 cctatagaag atataaactc tccagaacac atccagcgtc ggctgtccct ccagctcccc
     4381 atcctccacc acgcctacct cccatccatc ggaggcgtgg acgccagctg tgtcagcccc
     4441 tgcgtcagcc ccaccgccag cccccgccac agacatgtgc caccctcctt ccgagtcatg
     4501 gtctcgggcc tgtaactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     4561 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     4621 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     4681 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     4741 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     4801 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     4861 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4921 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4981 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5041 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5101 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5161 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5221 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5281 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5341 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5401 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5461 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5521 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5581 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5641 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     5701 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     5761 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     5821 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     5881 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5941 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6001 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6061 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6121 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6181 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6241 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     6301 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     6361 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     6421 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     6481 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     6541 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     6601 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     6661 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6721 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     6781 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6841 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6901 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6961 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7021 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7081 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7141 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7201 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7261 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7321 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7381 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7441 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7501 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7561 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7621 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7681 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7741 cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7981 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8041 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8101 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8161 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8221 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8281 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8341 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8401 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8461 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8521 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8581 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8641 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8701 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8761 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8821 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8881 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8941 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gt
//
