LOCUS       V015198                 8303 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015198
VERSION     V015198
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8303)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             908..3790
                     /gene="GABBR1"
                     /label="Gamma-aminobutyric acid type B receptor subunit 1"
                     /note="Gamma-aminobutyric acid type B receptor subunit 1
                     from Homo sapiens. Accession#: Q9UBS5"
     polyA_signal    3870..4094
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4140..4568
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4582..4911
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4978..5769
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5946..6079
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6116..6132)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6140..6156)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6164..6194)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6209..6230)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6518..7103)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7277..8134)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8135..8239)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg ttgctgctgc tgctactggc gccactcttc ctccgccccc cgggcgcggg
      961 cggggcgcat acccccaacg ccacctcaga aggttgccag atcatacacc cgccctggga
     1021 agggggcatc aggtaccggg gcctgactcg ggaccaggtg aaggctatca acttcctgcc
     1081 agtggactat gagattgagt atgtgtgccg gggggagcgc gaggtggtgg ggcccaaggt
     1141 ccgcaagtgc ctggccaacg gctcctggac agatatggac acacccagcc gctgtgtccg
     1201 aatctgctcc aagtcttatt tgaccctgga aaatgggaag gttttcctga cgggtgggga
     1261 cctcccagct ctggacggag cccgggcgga tttccggtgt gaccccgact tccatctggt
     1321 gggcagctcc cggagcatct gtagtcaggg ccagtggagc acccccaagc cccactgcca
     1381 ggtgaatcga acgccacact cagaacggcg cgcagtgtac atcggggcac tgtttcccat
     1441 gagcgggggc tggccagggg gccaggcctg ccagcccgcg gtggagatgg cgctggagga
     1501 cgtgaatagc cgcagggaca tcctgccgga ctatgagctc aagctcatcc accacgacag
     1561 caagtgtgat ccaggccaag ccaccaagta cctatatgag ctgctctaca acgaccctat
     1621 caagatcatc cttatgcctg gctgcagctc tgtctccacg ctggtggctg aggctgctag
     1681 gatgtggaac ctcattgtgc tttcctatgg ctccagctca ccagccctgt caaaccggca
     1741 gcgtttcccc actttcttcc gaacgcaccc atcagccaca ctccacaacc ctacccgcgt
     1801 gaaactcttt gaaaagtggg gctggaagaa gattgctacc atccagcaga ccactgaggt
     1861 cttcacttcg actctggacg acctggagga acgagtgaag gaggctggaa ttgagattac
     1921 tttccgccag agtttcttct cagatccagc tgtgcccgtc aaaaacctga agcgccagga
     1981 tgcccgaatc atcgtgggac ttttctatga gactgaagcc cggaaagttt tttgtgaggt
     2041 gtacaaggag cgtctctttg ggaagaagta cgtctggttc ctcattgggt ggtatgctga
     2101 caattggttc aagatctacg acccttctat caactgcaca gtggatgaga tgactgaggc
     2161 ggtggagggc cacatcacaa ctgagattgt catgctgaat cctgccaata cccgcagcat
     2221 ttccaacatg acatcccagg aatttgtgga gaaactaacc aagcgactga aaagacaccc
     2281 tgaggagaca ggaggcttcc aggaggcacc gctggcctat gatgccatct gggccttggc
     2341 actggccctg aacaagacat ctggaggagg cggccgttct ggtgtgcgcc tggaggactt
     2401 caactacaac aaccagacca ttaccgacca aatctaccgg gcaatgaact cttcgtcctt
     2461 tgagggtgtc tctggccatg tggtgtttga tgccagcggc tctcggatgg catggacgct
     2521 tatcgagcag cttcagggtg gcagctacaa gaagattggc tactatgaca gcaccaagga
     2581 tgatctttcc tggtccaaaa cagataaatg gattggaggg tcccccccag ctgaccagac
     2641 cctggtcatc aagacattcc gcttcctgtc acagaaactc tttatctccg tctcagttct
     2701 ctccagcctg ggcattgtcc tagctgttgt ctgtctgtcc tttaacatct acaactcaca
     2761 tgtccgttat atccagaact cacagcccaa cctgaacaac ctgactgctg tgggctgctc
     2821 actggcttta gctgctgtct tccccctggg gctcgatggt taccacattg ggaggaacca
     2881 gtttcctttc gtctgccagg cccgcctctg gctcctgggc ctgggcttta gtctgggcta
     2941 cggttccatg ttcaccaaga tttggtgggt ccacacggtc ttcacaaaga aggaagaaaa
     3001 gaaggagtgg aggaagactc tggaaccctg gaagctgtat gccacagtgg gcctgctggt
     3061 gggcatggat gtcctcactc tcgccatctg gcagatcgtg gaccctctgc accggaccat
     3121 tgagacattt gccaaggagg aacctaagga agatattgac gtctctattc tgccccagct
     3181 ggagcattgc agctccagga agatgaatac atggcttggc attttctatg gttacaaggg
     3241 gctgctgctg ctgctgggaa tcttccttgc ttatgagacc aagagtgtgt ccactgagaa
     3301 gatcaatgat caccgggctg tgggcatggc tatctacaat gtggcagtcc tgtgcctcat
     3361 cactgctcct gtcaccatga ttctgtccag ccagcaggat gcagcctttg cctttgcctc
     3421 tcttgccata gttttctcct cctatatcac tcttgttgtg ctctttgtgc ccaagatgcg
     3481 caggctgatc acccgagggg aatggcagtc ggaggcgcag gacaccatga agacagggtc
     3541 atcgaccaac aacaacgagg aggagaagtc ccggctgttg gagaaggaga accgtgaact
     3601 ggaaaagatc attgctgaga aagaggagcg tgtctctgaa ctgcgccatc aactccagtc
     3661 tcggcagcag ctccgctccc ggcgccaccc accgacaccc ccagaaccct ctgggggcct
     3721 gcccagggga ccccctgagc cccccgaccg gcttagctgt gatgggagtc gagtgcattt
     3781 gctttataag tgagaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
     3841 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3901 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     3961 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4021 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4081 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4141 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4201 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4261 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4321 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4381 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4441 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4501 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4561 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4741 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4801 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4861 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4921 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     4981 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5041 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5101 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5161 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5221 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5281 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5341 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5401 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5461 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5521 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5581 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5641 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5701 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5761 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5821 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5881 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5941 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6001 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6061 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6121 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6181 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6241 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6301 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6361 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6421 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6481 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6781 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6841 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6901 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6961 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7021 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta
     7081 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7141 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7201 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7261 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7321 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7381 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7441 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7501 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7561 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7621 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7681 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7741 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7801 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7861 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7921 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7981 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8041 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8101 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8161 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8221 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     8281 gagatctccc gatcccctat ggt
//
