LOCUS       V013436                 8317 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013436
VERSION     V013436
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        236..615
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        864..882
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             927..950
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             966..3110
                     /gene="BBS7"
                     /label="Bardet-Biedl syndrome 7 protein"
                     /note="Bardet-Biedl syndrome 7 protein from Homo sapiens.
                     Accession#: Q8IWZ6"
     CDS             3147..3863
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    3918..4142
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4188..4616
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4630..4959
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5026..5817
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5994..6127
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6164..6180)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6188..6204)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6212..6242)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6257..6278)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6566..7151)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7325..8182)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8183..8287)
                     /label="AmpR promoter"
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agctggctag
      901 cgtttaaact taagcttgcc accatggact acaaggacga tgacgacaag ggcgggggcg
      961 ggtctatgga tctgatttta aaccgaatgg attatctgca ggtgggagta acatctcaga
     1021 agactatgaa gctaattcct gcctcaagac acagagctac acaaaaggtg gttattggag
     1081 atcatgatgg ggtagttatg tgctttggca tgaagaaagg agaagcagca gcagtgttca
     1141 agactttacc cgggccgaag attgcaaggc tggaactggg aggggttatc aacacacctc
     1201 aggagaaaat ttttattgct gcagcatctg agattagagg cttcacaaaa agaggaaaac
     1261 agttcctctc ctttgaaaca aacctcactg aaagcattaa agctatgcac atatctggct
     1321 cagacctctt tctcagtgca agttacatct ataaccatta ttgtgactgc aaagaccaac
     1381 attattacct ttctggggat aaaatcaatg atgtgatctg ccttccagtg gaaagattat
     1441 ctcgtatcac acctgtattg gcctgccagg acagagtgct cagagtttta cagggatctg
     1501 atgtgatgta tgcagttgaa gttcctggac cccctactgt cttagcacta cacaatggaa
     1561 atggcggtga ctctggagaa gaccttttgt ttgggacatc agacggaaaa cttgcgctta
     1621 tacagattac tacatccaaa ccagtacgca agtgggaaat tcaaaatgag aaaaagagag
     1681 gaggtatttt gtgtattgac agctttgaca ttgtgggtga tggggttaaa gatttacttg
     1741 ttgggagaga tgacggaatg gtggaagtgt atagttttga taatgcaaat gaacctgttc
     1801 tacgatttga tcagatgttg tctgaaagcg tcacatctat ccagggtggt tgtgtaggaa
     1861 aagacagcta tgatgaaatc gtggtgtcca catattcagg ctgggttaca ggtctgacaa
     1921 cagagcccat tcataaggaa agtggaccag gagaagaact aaaaattaat caggagatgc
     1981 agaataaaat ttcttcctta cggaatgagt tggaacattt gcagtataag gtattgcagg
     2041 aaagagagaa ttatcaacag tcttctcaat caagcaaagc aaaatcagca gtaccttcct
     2101 ttggtataaa tgataaattt acactaaata aagatgatgc cagttacagc cttatcttag
     2161 aggtacagac tgcaatagat aatgtcttaa tacagagtga tgttccaata gatttacttg
     2221 atgtggataa aaattctgct gttgttagct ttagcagctg tgattctgag tcaaacgaca
     2281 acttccttct tgccacttat cggtgccagg cagatactac aaggctggaa ctcaagattc
     2341 gctcaattga aggccagtat ggcacactac aagcatatgt gactccaaga attcaaccca
     2401 aaacctgtca ggtccgccag taccacatca aacctctttc actccatcaa agaactcact
     2461 ttattgatca tgacagaccc atgaatacac tgaccctaac aggccagttc agttttgctg
     2521 aagttcactc ctgggtggtt ttttgtctgc ctgaagttcc agaaaaacct ccagcaggag
     2581 aatgtgtgac attttacttt cagaacacct ttctagatac acaacttgaa agtacctaca
     2641 gaaaaggaga gggagttttt aaatctgaca acatttctac tatctccatc ctaaaagatg
     2701 tgctttctaa agaagctaca aaaaggaaaa ttaacctcaa catatcatac gagataaatg
     2761 aagtatcagt caaacacact ttaaagctaa tccacccaaa gctggagtac cagttgcttt
     2821 tggctaagaa agtgcagtta attgatgctt taaaagaatt acagattcat gagggaaata
     2881 cgaactttct gataccagaa tatcactgta ttctagaaga ggcagatcac ctacaggaag
     2941 aatacaaaaa gcaacctgca catcttgaaa gactctatgg catgatcact gatcttttca
     3001 tagataaatt taagtttaaa ggcaccaatg taaaaactaa agtacccctt ctattggaaa
     3061 ttctggacag ttatgaccaa aatgcattga tttcattctt cgatgcagca ggcgggggcg
     3121 ggtctacgga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     3181 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3241 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3301 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     3361 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     3421 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     3481 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     3541 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     3601 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     3661 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3721 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     3781 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     3841 tcggcatgga cgagctgtac aagtaaagcg gccgctcgag tctagagggc ccgtttaaac
     3901 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3961 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4021 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4081 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4141 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4201 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4261 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4321 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4381 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4441 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4501 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4561 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     4621 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4681 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4741 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4801 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4861 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4921 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4981 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     5041 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5101 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5161 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     5221 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5281 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5341 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     5401 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     5461 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     5521 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     5581 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     5641 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     5701 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     5761 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     5821 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     5881 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     5941 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6061 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6121 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6181 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6241 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6301 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6361 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6421 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6481 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6541 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6601 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6901 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6961 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7021 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7081 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8281 ttccgcgcac atttccccga aaagtgccac ctgacgt
//
