LOCUS       V015138                 7846 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015138
VERSION     V015138
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    258..391
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(428..444)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(452..468)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(476..506)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(521..542)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(830..1418)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1592..2449)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2450..2554)
                     /label="AmpR promoter"
     enhancer        2820..3199
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3200..3403
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        3448..3466
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3498..3515
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3519..3542
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             3549..3560
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             3567..5834
                     /gene="IKBKB"
                     /label="Inhibitor of nuclear factor kappa-B kinase subunit
                     beta"
                     /note="Inhibitor of nuclear factor kappa-B kinase subunit
                     beta from Homo sapiens. Accession#: O14920"
     promoter        complement(5872..5890)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    5916..6140
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6186..6614
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6628..6957
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             7024..7815
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 acgtacgctc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
       61 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
      121 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
      181 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
      241 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      301 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      361 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
      421 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      481 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      541 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      601 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      661 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      721 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      781 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      841 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      901 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      961 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1021 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1081 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1141 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1201 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1261 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1321 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1381 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1441 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1501 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1561 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1621 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1681 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1741 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1801 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1861 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1921 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1981 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2041 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2101 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2161 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2221 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2281 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2341 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2401 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2461 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2521 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2581 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     2641 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     2701 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     2761 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     2821 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     2881 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     2941 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     3001 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     3061 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     3121 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     3181 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     3241 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     3301 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     3361 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
     3421 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttg
     3481 cgcgtatggc ttcgggtcat caccatcacc atcacggtga ctacaaggac gacgatgaca
     3541 aaggtgacat cgaaggtaga ggtcatatga gctggtcacc ttccctgaca acgcagacat
     3601 gcggggcctg ggaaatgaaa gagcgccttg ggacaggggg atttggaaat gtcatccgat
     3661 ggcacaatca ggaaacaggt gagcagattg ccatcaagca gtgccggcag gagctcagcc
     3721 cccggaaccg agagcggtgg tgcctggaga tccagatcat gagaaggctg acccacccca
     3781 atgtggtggc tgcccgagat gtccctgagg ggatgcagaa cttggcgccc aatgacctgc
     3841 ccctgctggc catggagtac tgccaaggag gagatctccg gaagtacctg aaccagtttg
     3901 agaactgctg tggtctgcgg gaaggtgcca tcctcacctt gctgagtgac attgcctctg
     3961 cgcttagata ccttcatgaa aacagaatca tccatcggga tctaaagcca gaaaacatcg
     4021 tcctgcagca aggagaacag aggttaatac acaaaattat tgacctagga tatgccaagg
     4081 agctggatca gggcagtctt tgcacatcat tcgtggggac cctgcagtac ctggccccag
     4141 agctactgga gcagcagaag tacacagtga ccgtcgacta ctggagcttc ggcaccctgg
     4201 cctttgagtg catcacgggc ttccggccct tcctccccaa ctggcagccc gtgcagtggc
     4261 attcaaaagt gcggcagaag agtgaggtgg acattgttgt tagcgaagac ttgaatggaa
     4321 cggtgaagtt ttcaagctct ttaccctacc ccaataatct taacagtgtc ctggctgagc
     4381 gactggagaa gtggctgcaa ctgatgctga tgtggcaccc ccgacagagg ggcacggatc
     4441 ccacgtatgg gcccaatggc tgcttcaagg ccctggatga catcttaaac ttaaagctgg
     4501 ttcatatctt gaacatggtc acgggcacca tccacaccta ccctgtgaca gaggatgaga
     4561 gtctgcagag cttgaaggcc agaatccaac aggacacggg catcccagag gaggaccagg
     4621 agctgctgca ggaagcgggc ctggcgttga tccccgataa gcctgccact cagtgtattt
     4681 cagacggcaa gttaaatgag ggccacacat tggacatgga tcttgttttt ctctttgaca
     4741 acagtaaaat cacctatgag actcagatct ccccacggcc ccaacctgaa agtgtcagct
     4801 gtatccttca agagcccaag aggaatctcg ccttcttcca gctgaggaag gtgtggggcc
     4861 aggtctggca cagcatccag accctgaagg aagattgcaa ccggctgcag cagggacagc
     4921 gagccgccat gatgaatctc ctccgaaaca acagctgcct ctccaaaatg aagaattcca
     4981 tggcttccat gtctcagcag ctcaaggcca agttggattt cttcaaaacc agcatccaga
     5041 ttgacctgga gaagtacagc gagcaaaccg agtttgggat cacatcagat aaactgctgc
     5101 tggcctggag ggaaatggag caggctgtgg agctctgtgg gcgggagaac gaagtgaaac
     5161 tcctggtaga acggatgatg gctctgcaga ccgacattgt ggacttacag aggagcccca
     5221 tgggccggaa gcagggggga acgctggacg acctagagga gcaagcaagg gagctgtaca
     5281 ggagactaag ggaaaaacct cgagaccagc gaactgaggg tgacagtcag gaaatggtac
     5341 ggctgctgct tcaggcaatt cagagcttcg agaagaaagt gcgagtgatc tatacgcagc
     5401 tcagtaaaac tgtggtttgc aagcagaagg cgctggaact gttgcccaag gtggaagagg
     5461 tggtgagctt aatgaatgag gatgagaaga ctgttgtccg gctgcaggag aagcggcaga
     5521 aggagctctg gaatctcctg aagattgctt gtagcaaggt ccgtggtcct gtcagtggaa
     5581 gcccggatag catgaatgcc tctcgactta gccagcctgg gcagctgatg tctcagccct
     5641 ccacggcctc caacagctta cctgagccag ccaagaagag tgaagaactg gtggctgaag
     5701 cacataacct ctgcaccctg ctagaaaatg ccatacagga cactgtgagg gaacaagacc
     5761 agagtttcac ggccctagac tggagctggt tacagacgga agaagaagag cacagctgcc
     5821 tggagcaggc ctcatgagcg gccgctcgag catgcatcta gagggcccta ttctatagtg
     5881 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5941 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6001 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6061 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6121 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     6181 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6241 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6301 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6361 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6421 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6481 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6541 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6601 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6661 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6721 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6781 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6841 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     6901 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     6961 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     7021 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     7081 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     7141 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     7201 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     7261 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     7321 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     7381 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     7441 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     7501 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     7561 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     7621 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     7681 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     7741 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     7801 cttgacgagt tcttctgagc gggactctgg ggtgcgaaat gagcga
//
