LOCUS       62056_5565        6264 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             898..924
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     promoter        complement(1816..1834)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1860..2084
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2130..2558
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2572..2902
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2969..3760
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3937..4070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4107..4123)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4131..4147)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4155..4185)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4200..4221)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4509..5097)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5271..6128)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6129..6233)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccgc caccatgtac
      901 ccatacgacg tcccagacta cgctggaagc ttggtaccga aacacgacgg gcgggtgaag
      961 atcggccact acattctggg tgacacgctg ggggtcggca ccttcggcaa agtgaaggtt
     1021 ggcaaacatg aattgactgg gcataaagta gctgtgaaga tactcaatcg acagaagatt
     1081 cggagccttg atgtggtagg aaaaatccgc agagaaattc agaacctcaa gcttttcagg
     1141 catcctcata taattaaact gtaccaggtc atcagtacac catctgatat tttcatggtg
     1201 atggaatatg tctcaggagg agagctattt gattatatct gtaagaatgg aaggctggat
     1261 gaaaaagaaa gtcggcgtct gttccaacag atcctttctg gtgtggatta ttgtcacagg
     1321 catatggtgg tccatagaga tttgaaacct gaaaatgtcc tgcttgatgc acacatgaat
     1381 gcaaagatag ctgattttgg tctttcaaac atgatgtcag atggtgaatt tttaagaaca
     1441 agttgtggct cacccaacta tgctgcacca gaagtaattt caggaagatt gtatgcaggc
     1501 ccagaggtag atatatggag cagtggggtt attctctatg ctttattatg tggaaccctt
     1561 ccatttgatg atgaccatgt gccaactctt tttaagaaga tatgtgatgg gatcttctat
     1621 acccctcaat atttaaatcc ttctgtgatt agccttttga aacatatgct gcaggtggat
     1681 cccatgaaga gggccacaat caaagatatc agggaacatg aatggtttaa acaggacctt
     1741 ccaaaatatc tctgagaatt ctgcagatat ccatcacact ggcggccgct cgagcatgca
     1801 tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
     1861 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     1921 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1981 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     2041 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     2101 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     2161 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     2221 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     2281 tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2341 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     2401 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2461 ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt
     2521 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     2581 ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct
     2641 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2701 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2761 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2821 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     2881 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     2941 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     3001 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     3061 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     3121 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     3181 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     3241 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     3301 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     3361 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     3421 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     3481 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     3541 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     3601 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     3661 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     3721 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     3781 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     3841 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     3901 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     3961 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4021 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     4081 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     4141 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4201 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4261 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4321 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4381 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4441 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4501 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4561 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4621 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4681 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     4741 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4801 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4861 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4921 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     4981 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5041 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5101 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5161 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5221 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5281 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5341 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5401 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5461 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5521 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5581 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5641 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5701 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5761 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5821 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5881 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5941 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6001 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6061 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6121 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     6181 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     6241 ccccgaaaag tgccacctga cgtc
//
