LOCUS       V016638                11774 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016638
VERSION     V016638
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11774)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..933
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             952..975
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1021..5886
                     /gene="KDM5A"
                     /label="Lysine-specific demethylase 5A"
                     /note="Lysine-specific demethylase 5A from Homo sapiens.
                     Accession#: P29375"
     promoter        complement(7326..7344)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    7370..7594
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7640..8068
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8082..8412
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             8479..9270
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    9447..9580
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(9665..9695)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(9710..9731)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10019..10607)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10781..11638)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11639..11743)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 atggcgtacc catacgatgt tccagattac gccaggatcc atcgaggagg agactacaag
      961 gacgacgatg acaagggagg acgatgtcga cgaattctgc agatggggat ccgtcgacgg
     1021 atggcgggcg tggggccggg gggctacgcg gcggagttcg tgccaccgcc agagtgcccc
     1081 gtctttgagc cgagttggga ggagttcaca gatccgctca gctttatcgg ccgcatccgg
     1141 cctttggcgg agaaaaccgg catctgcaaa attcggccgc ccaaggactg gcagcctcca
     1201 tttgcctgtg aagtaaaaag ctttcgtttc actccaagag tccagcgcct gaatgaactt
     1261 gaggcaatga ccagagtgag attggatttc ttggatcaac tagcaaaatt ttgggaactt
     1321 caaggatcta ctctgaagat ccctgtggta gagagaaaaa tcctggatct gtatgctttg
     1381 agcaagattg ttgccagcaa aggaggtttt gaaatggtca ccaaagagaa gaaatggtct
     1441 aaagtgggta gtcgcttggg atatctgcca ggaaaaggaa ctgggtctct tttgaagtca
     1501 cattatgaaa gaattctcta cccatatgag cttttccagt ctggtgtgag ccttatgggt
     1561 gtgcagatgc ctaatttaga tcttaaagaa aaagtggagc ctgaggttct cagcactgat
     1621 acccaaactt ccccagagcc aggcacaagg atgaacattc tgccgaagag aacaagacgt
     1681 gtgaagactc agtcagaatc tggagatgtg agtagaaaca cggaactgaa gaaacttcag
     1741 atttttgggg ctgggcccaa ggttgtgggc ttggcaatgg gaacaaaaga taaagaagat
     1801 gaggtcaccc gaagacgaaa agttaccaac aggtcagacg catttaacat gcaaatgaga
     1861 caacggaaag gcactctctc tgttaacttt gttgatctct atgtttgtat gttttgtggt
     1921 cggggaaaca atgaagataa attgcttttg tgtgatggat gtgatgacag ctatcataca
     1981 ttttgtctaa ttcctccact acctgatgtg cccaaaggag actggaggtg tcctaaatgt
     2041 gtcgccgagg aatgtagcaa acctcgagaa gcctttggat ttgaacaagc tgtacgagag
     2101 tatacacttc agagctttgg agagatggca gataatttta agtctgatta ttttaatatg
     2161 ccagtccata tggttcccac agaactagta gaaaaggaat tttggcggct ggtaagcagc
     2221 attgaagaag atgttattgt ggaatatgga gcagatatct cctcaaaaga ctttggaagt
     2281 ggatttccgg tgaaggatgg gcggagaaag attctgccag aagaagagga atatgcactt
     2341 tctggttgga atttgaataa catgcctgtc ctggaacagt ctgttcttgc acatattaat
     2401 gtggacatct ctggtatgaa agtgccgtgg ctctatgtgg gaatgtgctt ctcttctttt
     2461 tgctggcaca ttgaggatca ctggagttat tccatcaact acttgcactg gggggagcca
     2521 aagacatggt atggtgtgcc atctcatgct gcagagcaac tggaggaggt gatgagagag
     2581 ctggcccccg agttatttga atcccagcct gatcttctgc atcagttagt taccatcatg
     2641 aaccccaacg tgctaatgga gcatggtgtg cctgtgtaca ggaccaatca gtgtgctggc
     2701 gagtttgttg tgacatttcc tcgtgcctat cactctggat ttaaccaggg ctacaacttt
     2761 gctgaagctg tgaacttctg tactgctgac tggttgccca ttggacgtca atgtgtaaat
     2821 cattaccgac gcctaaggcg ccactgtgtc ttttcacacg aggaactaat tttcaagatg
     2881 gcagcagatc cagaatgctt agatgtgggg ctggctgcca tggtctgcaa agaattgact
     2941 ctcatgactg aagaagaaac acgattaaga gagtctgttg tacagatggg tgtcctgatg
     3001 tcagaagaag aagtgtttga acttgttcct gatgatgagc ggcagtgttc agcatgcaga
     3061 accacatgtt ttctctctgc tctcacatgt tcctgtaatc ctgagcggct tgtatgtctc
     3121 taccatccaa ctgatctgtg cccctgcccc atgcagaaga aatgtcttag atatcgctac
     3181 ccattagaag acctcccttc tctgctatat ggtgtaaaag tcagggcaca gtcctatgac
     3241 acttgggtca gtcgtgttac agaagcattg tctgctaact tcaaccacaa aaaagatttg
     3301 attgaattgc gagtaatgct ggaagatgct gaggatagga aatacccaga gaatgatctc
     3361 tttcgaaaac tcagggatgc tgtaaaagaa gctgagacct gtgcttctgt ggctcagctg
     3421 cttctgagca aaaagcagaa acacagacag agcccagata gtgggaggac tcggaccaaa
     3481 ctgacagtgg aagaattgaa ggcctttgtc caacaacttt ttagtcttcc gtgtgtcatc
     3541 agccaagctc ggcaagtaaa gaatctgcta gatgatgtgg aagagtttca tgaacgtgct
     3601 caggaggcca tgatggatga aaccccagat tcttccaaac tccagatgtt gatagatatg
     3661 ggctctagtc tctatgtgga actccctgaa ttaccacgac tgaagcaaga gctacaacag
     3721 gctcggtggt tggacgaagt aagactgacc ttatcagatc cgcaacaagt cactttggat
     3781 gtcatgaaga agctgataga ctctggggta gggttggcac cccaccatgc tgtggagaaa
     3841 gcaatggctg aactacagga gctccttaca gtctctgaac gatgggaaga aaaggctaag
     3901 gtctgcctac aggcaagacc gaggcacagt gtggcaagtt tagaaagcat tgtgaatgaa
     3961 gccaagaaca ttccagcctt tctacccaat gtgttgtcct tgaaagaagc cttacaaaag
     4021 gctcgagaat ggaccgctaa agtggaagct attcagagtg gcagcaatta cgcttatttg
     4081 gagcagcttg agagcttgtc tgcgaaagga cgccctattc ctgtgcgtct tgaagcactg
     4141 ccgcaagtgg aatcacaggt agcagcagca cgggcatgga gagaacggac tgggcggacg
     4201 tttcttaaga agaattctag ccatacattg ttacaggtgc tgagcccccg gaccgacatt
     4261 ggtgtatatg ggagtggcaa aaataggagg aaaaaagtaa aagaactaat agaaaaagaa
     4321 aaagaaaagg atctggacct ggagcctctg agtgatctgg aggaaggatt ggaggaaacc
     4381 agagatacag ccatggtggt ggcagttttc aaagaacggg agcaaaaaga gattgaagcc
     4441 atgcattctc tcagagcagc caacctagcc aagatgacaa tggtggaccg catagaagaa
     4501 gtaaaatttt gcatttgccg caagacagcc agtgggttta tgctacagtg tgagctctgc
     4561 aaagactggt tccataacag ctgtgttcct cttcctaaat caagttccca aaaaaaagga
     4621 tccagctggc aagctaaaga agtaaaattc ctttgccctc tttgtatgcg gtctcgaagg
     4681 cccaggctag agactattct gtcactcctg gtatcccttc agaagttgcc cgtacggttg
     4741 cctgaaggag aggccctgca gtgtttgaca gaacgtgcta tgagttggca agatagagcg
     4801 cggcaggctc tagccacaga tgaactatcc tctgccctgg ccaaactatc tgtgttgagc
     4861 cagcgtatgg tggaacaggc ggctcgagaa aaaactgaaa agatcatcag tgcagaactc
     4921 caaaaagcag ctgccaatcc agacttacag ggacacttac ctagtttcca gcagtctgct
     4981 tttaaccggg tggtgagcag tgtgtcatct tctcctcgac aaacaatgga ctatgatgat
     5041 gaagaaacag actctgatga agacattcga gagacatatg gctacgacat gaaggacaca
     5101 gccagtgtga agtcctctag tagtcttgaa cccaatcttt tttgtgatga agagattccc
     5161 atcaaatccg aggaggtggt gacccacatg tggacagcac cttcattttg tgcagagcat
     5221 gcttattctt ctgcttctaa gagttgttct caaggttcta gcaccccaag gaaacaacct
     5281 cggaagagcc ctttggtgcc ccgaagtttg gaacctccag tgctggagtt gtcacctgga
     5341 gctaaggcac aactggaaga acttatgatg gttggagatc tcctggaagt atctctggac
     5401 gagactcaac acatatggcg gattttgcag gccacacacc caccctctga agacagattc
     5461 ttgcatatca tggaggatga cagcatggaa gagaaaccac taaaagtgaa aggaaaggac
     5521 tcttcagaga agaaacggaa acggaagcta gaaaaggtag agcaactttt tggagaagga
     5581 aaacagaagt ccaaggagtt aaagaaaatg gacaaaccta gaaagaagaa attaaaatta
     5641 ggtgcagaca aatcaaagga gctgaataaa ctggccaaga aactagcaaa agaagaagag
     5701 agaaagaaaa agaaggagaa ggctgctgca gccaaagttg aacttgtgaa agagagcact
     5761 gaaaagaaaa gagagaaaaa ggtgctggac atcccctcaa agtatgactg gtcaggagca
     5821 gaggagtctg atgatgagaa tgctgtgtgc gcagcacaga actgccaaag gccctgcaag
     5881 gacaagggag ttgtatttgt aacggaagaa gagaagaata aaaaatatta gttttaaaag
     5941 tgtcctatgt gactgctttt ctaaaaaggt agactgggta caatgtgatg gtggctgtga
     6001 tgagtggttt catcaagttt gtgtgggtgt atctccagaa atggctgaaa atgaagatta
     6061 catctgtata aactgtgcaa agaagcaggg gccagttagc ccaggtccag caccacctcc
     6121 ttccttcata atgagctaca aactaccaat ggaggatctt aaagagacca gttagcagat
     6181 gcttggttag tttgggacat ggggggacat ggaccacatt gagaccttag tcatcaagta
     6241 gagtggttta tatcacttgg aatgttgctt ctaaagatga atggccttca gagaaagtcc
     6301 ccttagtgct ggcttcctct ttgcatggac tctgtgggtt acattgctct atcaacatat
     6361 ctatgcagag ggtgtcttct ttggtacaac agccaatatc tcatgtctcc tttgagtgtg
     6421 gtttactgca ttaaggccag atgcttaatt gagctctagg gtggctggtt agtattaata
     6481 cattggtgtg ctaacagggc atataggatg tggcttttgt ccagctgata gtagttagag
     6541 gcttacaact taggagcagc accaactgaa ggtgctaatt gcttggatct ccttcattgg
     6601 gatagttgga gagggattgg agtaccactt tccttccact gttacctggt acttaatgcc
     6661 ctaaagatac aactaggagt aacagggcca aagttatttc tgttagacgt caaggaatgg
     6721 tatcacagtc tattgacctc agcgatttgt gcttgtttgt gctagaagaa catcccaaat
     6781 aggagaacct ctcacaagct ggggcaggtc accttatctt tgtaagatga ggatatcatc
     6841 tagatcagaa atctgactag attggattct gaggagaaga acctactaca aggcaaggag
     6901 ccgttttttg gctttgaaaa gtcttgctgt cttgggtcta cattttaggg aagagcaggt
     6961 acatggatcc aggcttctgc caaaaaaaaa aagagaagaa gatgacgatg atgaccagtc
     7021 gtactatctt actgagccac agtgatgcat gcttttcggg gaaaacttca ttcacaagta
     7081 ttccagacac caggcttcag gcatggccat gagcaagacc agcaaataac agctttttgc
     7141 cttgcagccc tgaccccaat gtctgctgtt tccaacactg gtgatttcta actacggccc
     7201 acagcagatg ctgttgaata acaccatggc ttcatcagag gatgtggggt tgtagtacct
     7261 ctgggtgatg aagttgtttt agtaaatcca tttttaaaaa aaaaaaaaaa tctagagggc
     7321 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
     7381 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7441 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7501 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7561 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7621 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7681 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7741 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc
     7801 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7861 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7921 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7981 gtctattctt ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag
     8041 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     8101 gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca
     8161 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8221 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     8281 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8341 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8401 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     8461 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     8521 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     8581 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     8641 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     8701 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     8761 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     8821 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     8881 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     8941 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     9001 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     9061 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     9121 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     9181 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     9241 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     9301 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     9361 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     9421 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     9481 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9541 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     9601 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9661 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9721 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9781 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     9841 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     9901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     9961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10201 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10441 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    10801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    10861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    10921 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    10981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    11041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    11101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11641 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11701 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    11761 tgccacctga cgtc
//
