LOCUS       V016810                 7681 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016810
VERSION     V016810
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             891..3158
                     /gene="IKBKB"
                     /label="Inhibitor of nuclear factor kappa-B kinase subunit
                     beta"
                     /note="Inhibitor of nuclear factor kappa-B kinase subunit
                     beta from Homo sapiens. Accession#: O14920"
     CDS             3159..3182
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     polyA_signal    3248..3472
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3518..3946
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3960..4289
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4356..5147
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5324..5457
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5494..5510)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5518..5534)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5542..5572)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5587..5608)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5896..6481)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6655..7512)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7513..7617)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacgggcc ctctagaagt atgagctggt
      901 caccttccct gacaacgcag acatgtgggg cctgggaaat gaaagagcgc cttgggacag
      961 ggggatttgg aaatgtcatc cgatggcaca atcaggaaac aggtgagcag attgccatca
     1021 agcagtgccg gcaggagctc agcccccgga accgagagcg gtggtgcctg gagatccaga
     1081 tcatgagaag gctgacccac cccaatgtgg tggctgcccg agatgtccct gaggggatgc
     1141 agaacttggc gcccaatgac ctgcccctgc tggccatgga gtactgccaa ggaggagatc
     1201 tccggaagta cctgaaccag tttgagaact gctgtggtct gcgggaaggt gccatcctca
     1261 ccttgctgag tgacattgcc tctgcgctta gataccttca tgaaaacaga atcatccatc
     1321 gggatctaaa gccagaaaac atcgtcctgc agcaaggaga acagaggtta atacacaaaa
     1381 ttattgacct aggatatgcc aaggagctgg atcagggcag tctttgcaca tcattcgtgg
     1441 ggaccctgca gtacctggcc ccagagctac tggagcagca gaagtacaca gtgaccgtcg
     1501 actactggag cttcggcacc ctggcctttg agtgcatcac gggcttccgg cccttcctcc
     1561 ccaactggca gcccgtgcag tggcattcaa aagtgcggca gaagagtgag gtggacattg
     1621 ttgttagcga agacttgaat ggaacggtga agttttcaag ctctttaccc taccccaata
     1681 atcttaacag tgtcctggct gagcgactgg agaagtggct gcaactgatg ctgatgtggc
     1741 acccccgaca gaggggcacg gatcccacgt atgggcccaa tggctgcttc aaggccctgg
     1801 atgacatctt aaacttaaag ctggttcata tcttgaacat ggtcacgggc accatccaca
     1861 cctaccctgt gacagaggat gagagtctgc agagcttgaa ggccagaatc caacaggaca
     1921 cgggcatccc agaggaggac caggagctgc tgcaggaagc gggcctggcg ttgatccccg
     1981 ataagcctgc cactcagtgt atttcagacg gcaagttaaa tgagggccac acattggaca
     2041 tggatcttgt ttttctcttt gacaacagta aaatcaccta tgagactcag atctccccac
     2101 ggccccaacc tgaaagtgtc agctgtatcc ttcaagagcc caagaggaat ctcgccttct
     2161 tccagctgag gaaggtgtgg ggccaggtct ggcacagcat ccagaccctg aaggaagatt
     2221 gcaaccggct gcagcaggga cagcgagccg ccatgatgaa tctcctccga aacaacagct
     2281 gcctctccaa aatgaagaat tccatggctt ccatgtctca gcagctcaag gccaagttgg
     2341 atttcttcaa aaccagcatc cagattgacc tggagaagta cagcgagcaa accgagtttg
     2401 ggatcacatc agataaactg ctgctggcct ggagggaaat ggagcaggct gtggagctct
     2461 gtgggcggga gaacgaagtg aaactcctgg tagaacggat gatggctctg cagaccgaca
     2521 ttgtggactt acagaggagc cccatgggcc ggaagcaggg gggaacgctg gacgacctag
     2581 aggagcaagc aagggagctg tacaggagac taagggaaaa acctcgagac cagcgaactg
     2641 agggtgacag tcaggaaatg gtacggctgc tgcttcaggc aattcagagc ttcgagaaga
     2701 aagtgcgagt gatctatacg cagctcagta aaactgtggt ttgcaagcag aaggcgctgg
     2761 aactgttgcc caaggtggaa gaggtggtga gcttaatgaa tgaggatgag aagactgttg
     2821 tccggctgca ggagaagcgg cagaaggagc tctggaatct cctgaagatt gcttgtagca
     2881 aggtccgtgg tcctgtcagt ggaagcccgg atagcatgaa tgcctctcga cttagccagc
     2941 ctgggcagct gatgtctcag ccctccacgg cctccaacag cttacctgag ccagccaaga
     3001 agagtgaaga actggtggct gaagcacata acctctgcac cctgctagaa aatgccatac
     3061 aggacactgt gagggaacaa gaccagagtt tcacggccct agactggagc tggttacaga
     3121 cggaagaaga agagcacagc tgcctggagc aggcctcaga ctacaaggac gacgacgaca
     3181 aggaagctaa gtagcacgcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca
     3241 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     3301 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     3361 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     3421 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     3481 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     3541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3721 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3841 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     3961 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4021 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4081 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4141 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4201 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4261 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     4321 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     4381 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     4441 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     4501 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     4561 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     4621 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     4681 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     4741 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     4801 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     4861 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     4921 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     4981 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5041 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5101 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     5161 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     5221 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     5281 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     5341 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5401 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     5461 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     5521 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     5581 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     5641 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     5701 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     5761 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     5821 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     5881 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     5941 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6001 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6061 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6121 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6181 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6241 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6301 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     6361 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     6421 cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6481 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6541 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6601 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6661 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6721 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6781 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6841 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6901 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6961 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7021 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7081 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7141 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7201 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7261 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7321 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7381 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7441 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7501 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7561 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7621 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     7681 t
//
