LOCUS       V016812                 7731 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V016812
VERSION     V016812
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        202..581
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        582..785
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        830..848
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             954..3188
                     /gene="Chuk"
                     /label="Inhibitor of nuclear factor kappa-B kinase subunit
                     alpha"
                     /note="Inhibitor of nuclear factor kappa-B kinase subunit
                     alpha from Mus musculus. Accession#: Q60680"
     CDS             3195..3221
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     polyA_signal    3298..3522
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3568..3996
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4010..4339
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4406..5197
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5374..5507
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5544..5560)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5568..5584)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5592..5622)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5637..5658)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5946..6531)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6705..7562)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7563..7667)
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtaac ggccgccagt gtgctggaat tctgcagatc cggaattccc accatggagc
      961 ggcccccggg gctgcggccg ggcgcgggcg gcccctggga gatgcgggaa cggcttggca
     1021 ccggcggttt cgggaacgtc agtctgtacc agcaccggga acttgatctc aaaatagcaa
     1081 ttaagtcttg tcgtttagag ctaagttcca aaaacagaga gcgatggtgc catgaaatcc
     1141 agatcatgaa aaagttggac catgcgaatg ttgtaaaggc ctgtgatgtc cctgaggaat
     1201 tgaacttttt aattaacgat gtgcctcttc tggcaatgga gtactgttct ggaggggacc
     1261 tccggaagct actcaacaaa ccagaaaatt gttgtggact taaagaaagc cagatacttt
     1321 ctttactgag tgacatagga tctgggatcc gatatctgca tgaaaacaaa attatacatc
     1381 gagatctaaa acctgaaaat atagttcttc aagatgttgg tgggaagaca atacataaaa
     1441 taattgattt gggttatgcc aaagatgttg atcaaggaag tctctgtaca tcttttgtgg
     1501 gaacattgca gtatttggcc ccagagctct ttgaaaataa gccgtacaca gccactgtgg
     1561 attattggag ctttgggacc atggtgtttg aatgtattgc tggatatagg ccttttttgc
     1621 atcatctgca gccatttacc tggcatgaga agattaagaa gaaagatcca aagtgtatat
     1681 ttgcatgtga agagatgact ggagaagttc ggtttagtag ccatttacct cagccaaaca
     1741 gcctttgtag tttaatagta gagccaatgg aaagctggct ccaattgatg ctgaattggg
     1801 acccacagca gagaggggga cctattgatc ttactttgaa gcagccaaga tgttttgcat
     1861 taatggatca cattctcaat ttaaagatag tgcacatcct aaatatgact tctgcaaaaa
     1921 tcatttcttt tctgttacca tgtgatgaaa gtcttcattc actacagtct cgaattgagc
     1981 gtgaaacagg aataaataca ggttctcagg agcttctgtc agagacaggg atttctctgg
     2041 atcctcggaa accagcctct cagtgtgttc tagatggagt tagaggctgt gatagctaca
     2101 tggtttattt gtttgataaa agtaagactg tatatgaagg accatttgca tccagaagtt
     2161 tatctgattg tgtaaattat attgtacaag acagcaaaat acaactgcca attatacagc
     2221 tgcggaaagt atgggctgaa gcagtgcact acgtatctgg gctaaaggaa gactacagca
     2281 ggctcttcca gggacaaaga gcagcaatgt taagtcttct tagatataat gctaacttga
     2341 caaaaatgaa gaatactttg atctcagcat cacagcaact caaagctaaa ttggagtttt
     2401 ttcgaaaaag cattcagctt gacttggaga gatatagtga gcagatgact tatgggatat
     2461 cttcagaaaa aatgttaaaa gcatggaaag aaatggaaga aaaggccatt cactattctg
     2521 aggttggtgt cattggttat cttgaggatc aaattatgtc tttgcacact gaaatcatgg
     2581 agctgcagaa gagcccctat ggacgacgcc agggagactt gatggagtct ctggagcagc
     2641 gtgccattga tctctataag cagctaaagc acagacctcc tgatcacttg tacagcgaca
     2701 gcacagagat ggtgaagatc atcgtgcaca ccgtgcagag tcaggaccgt gttctcaagg
     2761 agctgtttgg tcacctgagc aagttgttgg gctgcaagca gaagattatt gatctactcc
     2821 ccaaggtgga agtggccctc agtaacatca aagaagctga caatactgtc atgtttatgc
     2881 agggaaagag gcagaaagaa atttggcacc tccttaaaat tgcctgtaca cagagttctg
     2941 cccgctctct tgtaggatac agtctagaag gcacagtaac ccctccagta tcagcatggc
     3001 tgccccctac attagcagac cgtgaacatc ctctgacatg tgtggtaact cctcaagatg
     3061 gagagacgtt agcacaaatg atagaagaaa atctgaactg tcttggccat ttaagtacta
     3121 ttattcgtga agcaaatgag gaccagagca gtagtttgat gagtcttgat tggagttggt
     3181 tagcagaata cccatacgat gttccagatt acgctagctt gtaggaattc gaggatccat
     3241 cacactggcg gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     3301 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     3361 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     3421 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     3481 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     3541 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     3601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     3661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     3721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     3781 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     3841 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     3901 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     3961 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     4021 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4081 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4141 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     4201 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4261 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     4321 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     4381 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4441 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4501 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4561 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4621 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4681 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4741 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4801 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4861 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4921 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4981 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5041 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5101 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     5161 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     5221 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     5281 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     5341 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     5401 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5461 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     5521 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     5581 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     5641 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     5701 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5761 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5821 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5881 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5941 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6001 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6061 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6121 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6181 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     6241 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     6301 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     6361 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     6421 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     6481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6541 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6601 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6661 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6721 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6781 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6841 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6901 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     6961 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7021 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7081 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7141 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7201 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7261 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7321 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7381 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7441 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7501 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7561 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7621 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7681 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg t
//
