LOCUS       40924_9801        7806 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Mammalian expression vector pCDNA-B-catenin, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7806)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7806)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             958..3300
                     /codon_start=1
                     /label=CTNNB1
                     /note="protein involved both in cell-cell adhesion as part
                     of the cadherin protein complex, and in gene transcription 
                     as part of the Wnt signaling pathway"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     promoter        complement(3358..3376)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    3402..3626
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3672..4100
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4114..4444
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4511..5302
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5479..5612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5649..5665)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5673..5689)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5697..5727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5742..5763)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6051..6639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6813..7670)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7671..7775)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat cnnnnnnatg
      961 gctactcaag ctgatttgat ggagttggac atggccatgg aaccagacag aaaagcggct
     1021 gttagtcact ggcagcaaca gtcttacctg gactctggaa tccattctgg tgccactacc
     1081 acagctcctt ctctgagtgg taaaggcaat cctgaggaag aggatgtgga tacctcccaa
     1141 gtcctgtatg agtgggaaca gggattttct cagtccttca ctcaagaaca agtagctgat
     1201 attgatggac agtatgcaat gactcgagct cagagggtac gagctgctat gttccctgag
     1261 acattagatg agggcatgca gatcccatct acacagtttg atgctgctca tcccactaat
     1321 gtccagcgtt tggctgaacc atcacagatg ctgaaacatg cagttgtaaa cttgattaac
     1381 tatcaagatg atgcagaact tgccacacgt gcaatccctg aactgacaaa actgctaaat
     1441 gacgaggacc aggtggtggt taataaggct gcagttatgg tccatcagct ttctaaaaag
     1501 gaagcttcca gacacgctat catgcgttct cctcagatgg tgtctgctat tgtacgtacc
     1561 atgcagaata caaatgatgt agaaacagct cgttgtaccg ctgggacctt gcataacctt
     1621 tcccatcatc gtgagggctt actggccatc tttaagtctg gaggcattcc tgccctggtg
     1681 aaaatgcttg gttcaccagt ggattctgtg ttgttttatg ccattacaac tctccacaac
     1741 cttttattac atcaagaagg agctaaaatg gcagtgcgtt tagctggtgg gctgcagaaa
     1801 atggttgcct tgctcaacaa aacaaatgtt aaattcttgg ctattacgac agactgcctt
     1861 caaattttag cttatggcaa ccaagaaagc aagctcatca tactggctag tggtggaccc
     1921 caagctttag taaatataat gaggacctat acttacgaaa aactactgtg gaccacaagc
     1981 agagtgctga aggtgctatc tgtctgctct agtaataagc cggctattgt agaagctggt
     2041 ggaatgcaag ctttaggact tcacctgaca gatccaagtc aacgtcttgt tcagaactgt
     2101 ctttggactc tcaggaatct ttcagatgct gcaactaaac aggaagggat ggaaggtctc
     2161 cttgggactc ttgttcagct tctgggttca gatgatataa atgtggtcac ctgtgcagct
     2221 ggaattcttt ctaacctcac ttgcaataat tataagaaca agatgatggt ctgccaagtg
     2281 ggtggtatag aggctcttgt gcgtactgtc cttcgggctg gtgacaggga agacatcact
     2341 gagcctgcca tctgtgctct tcgtcatctg accagccgac accaagaagc agagatggcc
     2401 cagaatgcag ttcgccttca ctatggacta ccagttgtgg ttaagctctt acacccacca
     2461 tcccactggc ctctgataaa ggctactgtt ggattgattc gaaatcttgc cctttgtccc
     2521 gcaaatcatg cacctttgcg tgagcagggt gccattccac gactagttca gttgcttgtt
     2581 cgtgcacatc aggataccca gcgccgtacg tccatgggtg ggacacagca gcaatttgtg
     2641 gagggggtcc gcatggaaga aatagttgaa ggttgtaccg gagcccttca catcctagct
     2701 cgggatgttc acaaccgaat tgttatcaga ggactaaata ccattccatt gtttgtgcag
     2761 ctgctttatt ctcccattga aaacatccaa agagtagctg caggggtcct ctgtgaactt
     2821 gctcaggaca aggaagctgc agaagctatt gaagctgagg gagccacagc tcctctgaca
     2881 gagttacttc actctaggaa tgaaggtgtg gcgacatatg cagctgctgt tttgttccga
     2941 atgtctgagg acaagccaca agattacaag aaacggcttt cagttgagct gaccagctct
     3001 ctcttcagaa cagagccaat ggcttggaat gagactgctg atcttggact tgatattggt
     3061 gcccagggag aaccccttgg atatcgccag gatgatccta gctatcgttc ttttcactct
     3121 ggtggatatg gccaggatgc cttgggtatg gaccccatga tggaacatga gatgggtggc
     3181 caccaccctg gtgctgacta tccagttgat gggctgccag atctggggca tgcccaggac
     3241 ctcatggatg ggctgcctcc aggtgacagc aatcagctgg cctggtttga tactgacctg
     3301 taannnnnnt atccatcaca ctggcggccg ctcgagcatg catctagagg gccctattct
     3361 atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     3421 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3481 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3541 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3601 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3661 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3721 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3781 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
     3841 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3901 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     3961 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4021 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
     4081 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4141 ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4201 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4261 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4321 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4381 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4441 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4501 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4561 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4621 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4681 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4741 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4801 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4861 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4921 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4981 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5041 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5101 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5161 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5221 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     5281 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     5341 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     5401 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     5461 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5521 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5581 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     5641 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5701 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5761 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5821 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5881 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5941 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6001 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6061 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6241 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6481 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6541 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6601 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6661 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6721 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6781 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6841 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6901 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6961 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7021 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7081 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7141 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7201 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7261 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7321 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7381 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7441 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7501 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7561 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7621 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7681 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7741 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7801 gacgtc
//
