LOCUS       V015158                 9321 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015158
VERSION     V015158
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     protein_bind    complement(2056..2085)
                     /label="NFAT binding site"
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        2093..2206
                     /label="IL-2 promoter"
                     /note="minimal promoter from the human interleukin-2 gene"
     CDS             2254..3903
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             3979..5406
                     /label="tdTomato"
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     misc_feature    5416..6004
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6078..6311
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6383..6517
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6523..6658
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6696..6712)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6720..6736)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6744..6774)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6789..6810)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7098..7686)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7860..8717)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8718..8822)
                     /label="AmpR promoter"
     primer_bind     9296..9312
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtaaggatc tgcgatcgct ccggtgcccg tcagtacgcc ttctgtatga
     1981 aacagttttt cctccacgcc ttctgtatga aacagttttt cctccacgcc ttctgtatga
     2041 aacagttttt cctccacgcc ttctgtatga aacagttttt cctcctcgag gacattttga
     2101 cacccccata atatttttcc agaattaaca gtataaattg catctcttgt tcaagagttc
     2161 cctatcactc tctttaatca ctactcacag taacctcaac tcctgcccaa gcttcaagct
     2221 gtgaccggcg cctactctag agctagcgaa ttcatggaag atgccaaaaa cattaagaag
     2281 ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct gcacaaagcc
     2341 atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca tatcgaggtg
     2401 gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc tatgaagcgc
     2461 tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt gcagttcttc
     2521 atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa cgacatctac
     2581 aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt attcgtgagc
     2641 aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat acaaaagatc
     2701 atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac cttcgtgact
     2761 tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt cgaccgggac
     2821 aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa gggcgtagcc
     2881 ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat cttcggcaac
     2941 cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg cttcggcatg
     3001 ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta ccgcttcgag
     3061 gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct gctggtgccc
     3121 acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct aagcaacttg
     3181 cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc cgtggccaaa
     3241 cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac cagcgccatt
     3301 ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt gcccttcttc
     3361 gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca gcgcggcgag
     3421 ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga ggctacaaac
     3481 gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg ggacgaggac
     3541 gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg ctaccaggta
     3601 gccccagccg aactggagag catcctgctg caacacccca acatcttcga cgccggggtc
     3661 gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt gctggaacac
     3721 ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt tacaaccgcc
     3781 aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact gaccggcaag
     3841 ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg caagatcgcc
     3901 gtgttagcgg ccgctgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     3961 cccggccctt ccggaaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc
     4021 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     4081 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     4141 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     4201 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     4261 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     4321 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     4381 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     4441 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     4501 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     4561 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4621 tacgagcgct ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggt
     4681 agcggcagct ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc
     4741 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     4801 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
     4861 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
     4921 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
     4981 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
     5041 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
     5101 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
     5161 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
     5221 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
     5281 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
     5341 gaacagtacg agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg
     5401 tacaagtaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5461 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5521 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5581 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5641 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5701 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5761 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5821 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5881 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5941 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6001 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6061 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     6121 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6181 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6241 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6301 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6361 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6421 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6481 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6541 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6601 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6661 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6721 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6781 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6841 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6901 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6961 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7021 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7081 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7141 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7201 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7261 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7321 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7381 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7441 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7501 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7561 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7621 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7681 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7741 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7801 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7861 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7921 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7981 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8041 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8101 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8161 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8221 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8281 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8341 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8401 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8461 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8521 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8581 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8641 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8701 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8761 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8821 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8881 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     8941 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     9001 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     9061 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     9121 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     9181 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     9241 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     9301 acgacggcca gtgccaagct g
//
