LOCUS       V014512                 9590 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014512
VERSION     V014512
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             50..91
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    168..284
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        374..577
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             657..683
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             684..2048
                     /gene="METTL14"
                     /label="N6-adenosine-methyltransferase non-catalytic
                     subunit"
                     /note="N6-adenosine-methyltransferase non-catalytic subunit
                     from Pongo abelii. Accession#: Q5R5N4"
     promoter        2093..2304
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             2317..2585
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2641..3306
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             3376..3429
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             3430..4026
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4030..4618
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4692..4925
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4997..5131
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5158..5293
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(5326..5342)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5350..5366)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5374..5404)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5419..5440)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5728..6316)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6490..7347)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7348..7452)
                     /label="AmpR promoter"
     primer_bind     7926..7942
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7957..8183
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    8411..8536
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    9029..9262
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             9447..9491
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
ORIGIN
        1 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
       61 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
      121 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
      181 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      241 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat actagtatta
      301 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      361 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      421 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      481 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      541 taggcgtgta cggtgggagg tttatataag cagagctcgt ttagtgaacc gtcagatcgc
      601 ctggagacgc catccacgct gttttgacct ccatagaaga ttctagaacc accatgtacc
      661 catacgatgt tccagattac gctgatagcc gcttgcagga gatccgggag cggcagaagt
      721 tacggcgaca gctcctcgcg cagcagttgg gagctgaaag tgccgacagc attggtgccg
      781 tgttaaatag caaagatgag cagagagaaa ttgctgaaac aagagaaact tgcagggctt
      841 cctatgatac ctctgctcca aatgcaaaac gtaagtatct ggatgaagga gagacagatg
      901 aagacaaaat ggaagaatat aaggatgaac tagaaatgca acaggatgaa gaaaatttgc
      961 catatgaaga agagatttac aaagattcta gtacttttct taagggaaca cagagcttaa
     1021 atccccataa tgattactgc caacattttg tagacactgg acatagacct cagaatttca
     1081 tcagggatgt aggtttagct gacagatttg aagaatatcc taaactgagg gagctcatca
     1141 ggctaaagga tgagttaata gctaaatcta acactcctcc catgtactta caagccgata
     1201 tagaagcctt tgacatcaga gaactaacac ccaaatttga tgtgattctt ctggaacccc
     1261 ctttagaaga atattacaga gaaactggca tcactgctaa tgaaaaatgc tggacttggg
     1321 atgatattat gaagttagaa attgatgaga ttgcagcacc tcgatcattt atttttctct
     1381 ggtgtggttc tggggagggg ttggaccttg gaagagtgtg tttacgaaaa tggggttaca
     1441 gaagatgtga agatatttgt tggattaaaa ccaataaaaa caatcctggg aagactaaga
     1501 ctttagatcc aaaggctgtc tttcagagaa caaaggaaca ctgcctcatg gggatcaaag
     1561 gaactgtgaa gcgtagcaca gacggggact tcattcatgc taatgttgac attgacttaa
     1621 ttatcacaga agaacctgaa attggcaata tagaaaaacc tgtagaaatt tttcatataa
     1681 ttgagcattt ttgtcttggt agaagacgcc ttcatctatt tggaagagat agtacaattc
     1741 gaccaggctg gctcacagtt ggaccaacgc ttacaaatag caactacaat gcagaaacat
     1801 atgcatccta tttcagtgct cctaattcct acttgactgg ttgtacagaa gaaattgaga
     1861 gacttcgacc aaaatcgcct cctcccaaat ctaaatctga ccgaggaggt ggagctccca
     1921 gaggtggagg aagaggtgga acttctgctg gccgtggacg agaaagaaat agatctaact
     1981 tccgaggaga aagaggtggc tttagagggg gccgtggagg agcacacaga ggtggctttc
     2041 cacctcgata agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
     2101 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
     2161 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2221 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2281 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
     2341 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
     2401 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
     2461 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
     2521 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
     2581 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
     2641 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
     2701 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
     2761 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
     2821 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
     2881 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
     2941 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
     3001 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
     3061 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
     3121 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
     3181 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
     3241 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
     3301 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
     3361 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
     3421 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     3481 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     3541 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     3601 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     3661 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     3721 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     3781 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     3841 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     3901 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     3961 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     4021 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4081 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4141 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4201 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4261 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4321 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4381 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     4441 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4501 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4561 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4621 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4681 aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg
     4741 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4801 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4861 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4921 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     4981 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5041 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5101 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     5161 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     5221 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     5281 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc
     5341 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     5401 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5461 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5521 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     5581 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     5641 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     5701 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     5761 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     5821 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     5881 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     5941 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6001 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6061 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6121 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     6181 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6241 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6301 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6361 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     6421 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     6481 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6541 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6601 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6661 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6721 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6781 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6841 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6901 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6961 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7021 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7081 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7141 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7201 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7261 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7321 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7381 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7441 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     7501 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     7561 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     7621 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     7681 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     7741 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     7801 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     7861 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     7921 acgttgtaaa acgacggcca gtgccaagct gacgcgtgta gtcttatgca atactcttgt
     7981 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     8041 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     8101 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     8161 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     8221 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8281 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8341 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     8401 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     8461 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     8521 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     8581 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     8641 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     8701 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     8761 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8821 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccac
     8881 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     8941 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     9001 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     9061 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     9121 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     9181 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     9241 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     9301 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     9361 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     9421 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     9481 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     9541 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
//
