LOCUS       V015176                10785 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015176
VERSION     V015176
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             3718..5367
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             5443..6870
                     /label="tdTomato"
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
     misc_feature    6880..7468
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7542..7775
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7847..7981
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      7987..8122
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(8208..8238)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8253..8274)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8562..9150)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9324..10181)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10182..10286)
                     /label="AmpR promoter"
     primer_bind     10760..10776
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtaaggatc tgcgatcgct ccggtgcccg tcagtagtag ccccctcttt
     1981 gcccctcact gagccctact ggatgttctt ggttgtgtga cagtttcccc atctattaaa
     2041 cagaaacccc tatagcagag gggaggatga ggttggaaaa tcaggagcat tgttattcta
     2101 ttcttgtggg atcggggaag cagacatctg ggtggatgtt tggggaatgc tgggctcagt
     2161 tgaggaagta ggggggcccc tggggcttac agggactgga agctctgagc tggccagagg
     2221 gatgttgcaa tcctgccagg gtcttgtcta tgctgtccct ttcacaacca tccccctacc
     2281 gccaggctga cacgtggttg tgggggcaca aggccagccg aactagagtc tgaggctggg
     2341 ctgaggacac cctccccatc agctgccagg gtcactggcg gtcaaaggca gctggtgggg
     2401 aaggaattgg actccagccc tgggggacgg atgtggtgat ggtgggaagc aggcttggtg
     2461 ccaggagggg catcagaggg tgaataagag cagatagagt gtttggggga ggtagccagc
     2521 caaagggggt gaggcccggt ggaagggaag aaggggcata cactcagagc tttgcagctg
     2581 aagggctagc gcagagccag gattcacgcc ctggcaattt gactccagaa tcctaacctt
     2641 aacccagaag cacggcttca agcccctgga aaccacaata cctgtggcag ccagggggag
     2701 gtgctggaat ctcatttcac atgtggggag ggggctcccc tgtgctcaag gtcacaacca
     2761 aagaggaagc tgtgattaaa acccaggtcc catttgcaaa gcctcgactt ttagcaggtg
     2821 catcatactg ttcccacccc tcccatccca cttctgtcca gccgcctagc cccactttct
     2881 tttttttctt tttttgagac agtctccctc ttgctgaggc tggagtgcag tggcgagatc
     2941 tcggctcact gtaacctccg cctcccgggt tcaagcgatt ctcctgcctc agcctcccaa
     3001 gtagctagga ttacaggcgc ccgccaccac gcctggctaa cttttgtatt tttagtagag
     3061 atggggtttc accatgttgg ccaggctggt ctcaaactcc tgaccttaag tgattcgccc
     3121 actgtggcct cccaaagtgc tgggattaca ggcgtgagct accgccccca gcccctccca
     3181 tcccacttct gtccagcccc ctagccctac tttctttctg ggatccagga gtccagatcc
     3241 ccagccccct ctccagatta cattcatcca ggcacaggaa aggacagggt caggaaagga
     3301 ggactctggg cggcagcctc cacattcccc ttccacgctt ggcccccaga atggaggagg
     3361 gtgtctgtat tactgggcga ggtgtcctcc cttcctgggg actgtggggg gtggtcaaaa
     3421 gacctctatg ccccacctcc ttcctccctc tgccctgctg tgcctggggc agggggagaa
     3481 cagcccacct cgtgactggg ggctggccca gcccgcccta tccctggggg agggggcggg
     3541 acagggggag ccctataatt ggacaagtct gggatccttg agtcctactc agccccagcg
     3601 gaggtgaagg acgtccttcc ccaggagccg actggccaat cacaggcagg aagatgaagg
     3661 ttctcgagga ccaagcttca agctgtgacc ggcgcctact ctagagctag cgaattcatg
     3721 gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc
     3781 gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc
     3841 tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt
     3901 cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc
     3961 agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct
     4021 gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc
     4081 cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag
     4141 aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc
     4201 caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc
     4261 gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt
     4321 accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat
     4381 gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg
     4441 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
     4501 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
     4561 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
     4621 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
     4681 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
     4741 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
     4801 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
     4861 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
     4921 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
     4981 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
     5041 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
     5101 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
     5161 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
     5221 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
     5281 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
     5341 gccaagaagg gcggcaagat cgccgtgtta gcggccgctg agggcagagg aagtcttcta
     5401 acatgcggtg acgtggagga gaatcccggc ccttccggaa ccatggtgag caagggcgag
     5461 gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac
     5521 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     5581 ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc
     5641 atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg
     5701 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg
     5761 accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc
     5821 ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     5881 tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg
     5941 aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag
     6001 cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac
     6061 gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg
     6121 gggcatggca ccggcagcac cggtagcggc agctccggca ccgcctcctc cgaggacaac
     6181 aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
     6241 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     6301 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     6361 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
     6421 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     6481 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     6541 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     6601 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     6661 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     6721 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     6781 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     6841 ttcctgtacg gcatggacga gctgtacaag taagtcgaca atcaacctct ggattacaaa
     6901 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     6961 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7021 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7081 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7141 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7201 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7261 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7321 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7381 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7441 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     7501 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     7561 ccaacgaaaa taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     7621 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7681 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7741 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     7801 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     7861 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7921 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     7981 ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8041 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     8101 tgaggaggct tttttggagg cctagacttt tgcagagacg gcccaaattc gtaatcatgg
     8161 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8221 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8281 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8341 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8401 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8461 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8521 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8581 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8641 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8701 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8761 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8821 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8881 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8941 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9001 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9061 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9121 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9181 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9241 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9301 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9361 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9421 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9481 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9541 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9601 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9661 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9721 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9781 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9841 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9901 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9961 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10021 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10081 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10141 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10201 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10261 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    10321 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    10381 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    10441 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    10501 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
    10561 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    10621 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    10681 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    10741 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctg
//
