LOCUS       V021905                 9512 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021905
VERSION     V021905
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        1960..2171
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             2184..2452
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             2486..4447
                     /label="TCF3(NM_003200)"
                     /note="TCF3(NM_003200)"
                     /gene="TCF3"
     promoter        4481..4980
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             5001..5600
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5607..6195
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6269..6502
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6574..6695
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6714..6849
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6887..6903)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6911..6927
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6935..6965)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6980..7001
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7289..7877)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8048..8908)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8909..9013)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9487..9503
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagag ccaccatgaa ccagccgcag aggatggcgc ctgtgggcac
     2521 agacaaggag ctcagtgacc tcctggactt cagcatgatg ttcccgctgc ctgtcaccaa
     2581 cgggaagggc cggcccgcct ccctggccgg ggcgcagttc ggaggttcag gtcttgagga
     2641 ccggcccagc tcaggctcct ggggcagcgg cgaccagagc agctcctcct ttgaccccag
     2701 ccggaccttc agcgagggca cccacttcac tgagtcgcac agcagcctct cttcatccac
     2761 attcctggga ccgggactcg gaggcaagag cggtgagcgg ggcgcctatg cctccttcgg
     2821 gagagacgca ggcgtgggcg gcctgactca ggctggcttc ctgtcaggcg agctggccct
     2881 caacagcccc gggcccctgt ccccttcggg catgaagggg acctcccagt actacccctc
     2941 ctactccggc agctcccggc ggagagcggc agacggcagc ctagacacgc agcccaagaa
     3001 ggtccggaag gtcccgccgg gtcttccatc ctcggtgtac ccacccagct caggtgagga
     3061 ctacggcagg gatgccaccg cctacccgtc cgccaagacc cccagcagca cctatcccgc
     3121 ccccttctac gtggcagatg gcagcctgca cccctcagcc gagctctgga gtcccccggg
     3181 ccaggcgggc ttcgggccca tgctgggtgg gggctcatcc ccgctgcccc tcccgcccgg
     3241 tagcggcccg gtgggcagca gtggaagcag cagcacgttt ggtggcctgc accagcacga
     3301 gcgtatgggc taccagctgc atggagcaga ggtgaacggt gggctcccat ctgcatcctc
     3361 cttctcctca gcccccggag ccacgtacgg cggcgtctcc agccacacgc cgcctgtcag
     3421 cggggccgac agcctcctgg gctcccgagg gaccacagct ggcagctccg gggatgccct
     3481 cggcaaagca ctggcctcga tctactcccc ggatcactca agcaataact tctcgtccag
     3541 cccttctacc cccgtgggct ccccccaggg cctggcagga acgtcacagt ggcctcgagc
     3601 aggagccccc ggtgccttat cgcccagcta cgacgggggt ctccacggcc tgcagagtaa
     3661 gatagaagac cacctggacg aggccatcca cgtgctccgc agccacgccg tgggcacagc
     3721 cggcgacatg cacacgctgc tgcctggcca cggggcgctg gcctcaggtt tcaccggccc
     3781 catgtcactg ggcgggcggc acgcaggcct ggttggaggc agccaccccg aggacggcct
     3841 cgcaggcagc accagcctca tgcacaacca cgcggccctc cccagccagc caggcaccct
     3901 ccctgacctg tctcggcctc ccgactccta cagtgggcta gggcgagcag gtgccacggc
     3961 ggccgccagc gagatcaagc gggaggagaa ggaggacgag gagaacacgt cagcggctga
     4021 ccactcggag gaggagaaga aggagctgaa ggccccccgg gcccggacca gcccagacga
     4081 ggacgaggac gaccttctcc ccccagagca gaaggccgag cgggagaagg agcgccgggt
     4141 ggccaataac gcccgggagc ggctgcgggt ccgtgacatc aacgaggcct ttaaggagct
     4201 ggggcgcatg tgccaactgc acctcaacag cgagaagccc cagaccaaac tgctcatcct
     4261 gcaccaggct gtctcggtca tcctgaactt ggagcagcaa gtgcgagagc ggaacctgaa
     4321 tcccaaagca gcctgtttga aacggcgaga agaggaaaag gtgtcaggtg tggttggaga
     4381 cccccagatg gtgctttcag ctccccaccc aggcctgagc gaagcccaca accccgccgg
     4441 gcacatgtga gctagcgaat tcgaaggatc cgcggccgct gggtagggga ggcgcttttc
     4501 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     4561 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     4621 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     4681 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     4741 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     4801 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     4861 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     4921 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     4981 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     5041 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     5101 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     5161 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     5221 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     5281 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     5341 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     5401 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     5461 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     5521 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     5581 cccgcaagcc cggtgcctga gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     5641 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     5701 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     5761 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     5821 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     5881 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5941 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6001 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6061 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6121 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6181 gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6241 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaaataa
     6301 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6361 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6421 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6481 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6541 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6601 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6661 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6781 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     6841 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc
     6901 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6961 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7021 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     7081 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     7141 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7201 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7261 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7321 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7381 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7441 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7501 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7561 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7621 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7681 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7741 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7801 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7861 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7921 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7981 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     8041 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     8101 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     8161 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8221 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8281 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8341 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8401 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8461 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8521 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8581 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8641 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8701 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8761 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8821 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8881 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8941 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9001 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     9061 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     9121 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9181 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9241 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9301 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     9361 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9421 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9481 gacgttgtaa aacgacggcc agtgccaagc tg
//
