LOCUS       V034637                12281 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V034637
VERSION     V034637
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        1960..2171
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             2184..2452
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             2543..2566
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             3974..4030
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4049..7237
                     /label="ITGA8(NM_003638)"
                     /note="ITGA8(NM_003638)"
                     /gene="ITGA8"
     promoter        7250..7749
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             7770..8369
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    8376..8964
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9038..9271
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    9343..9464
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      9483..9618
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9656..9672)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9680..9696
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9704..9734)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9749..9770
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10058..10646)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10817..11677)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11678..11782)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     12256..12272
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagaa tggctctgcc agtgacagct ctgctgcttc cactggcact
     2521 gttgctgcat gccgcaaggc ctgactacaa agatgacgac gacaagggag gtggcggatc
     2581 tgagatcgtc ttgacacagt cacctgccac cttgtctctg tcacctggcg agagagccac
     2641 cttgagctgt agagccagcc aagacatcag caagtatctg aactggtatc agcagaagcc
     2701 aggtcaggca cctagactgc tgatctacca cacctctcgg cttcactccg gcattccaga
     2761 tcggttctct ggttccggca gcggcacaga cttcactctg accatcagca ggctggagcc
     2821 agaggacatc gccgtgtact actgtcaaca gggcaacaca ctgccataca ccttcggtca
     2881 gggaaccaag ctggaaatcg gtggcggcgg aagcggcgga ggtggatctg gaggcggtgg
     2941 aagccaagtc acattgagag aaagcggtcc agctctggtg aagcctactc agaccttgac
     3001 cttgacatgc accttctccg gcgttagcct gcctgactat ggagtgagct ggatcagaca
     3061 gccacctggt aaggctctgg aatggctcgc cgtgatctgg ggcagcgaga caacctacta
     3121 caacagcgca ctgaagtcca gactgaccat ctccaaagac acctccaaga accaggtggt
     3181 gttgaccatg acaaacatgg acccagtgga caccgctacc tactattgtg ccaagcacta
     3241 ctactacggt ggcagttacg ccatggatta ctggggtcag ggcaccactg tcaccgtgtc
     3301 cagcaccacc actccagcac ctaggcctcc aaccccagct ccaacaatcg ccagtcagcc
     3361 actgtctctt agacctgagg cctgcagacc agcagcaggt ggtgccgtgc ataccagagg
     3421 cttggacttc gcctgtgaca tctacatctg ggctccattg gctggcacat gcggcgtact
     3481 gctgctgagc ctggtcatca cactgtactg caagcgcggt cggaagaagt tgctgtacat
     3541 cttcaagcag ccatttatgc ggcctgtcca gaccacacaa gaagaagatg gatgcagctg
     3601 cagatttcca gaagaagaag agggcggttg tgagttgcgg gtcaagttct ccagatctgc
     3661 tgacgctcca gcttaccagc agggccagaa tcagctgtat aacgagttga acctgggcag
     3721 aagggaagaa tacgatgtgc tggataagag gcgcggaagg gaccctgaga tgggtggtaa
     3781 gcctagacgg aagaatccac aagagggtct gtacaatgag ctgcagaaag ataagatggc
     3841 cgaggcctac tccgagatcg gcatgaaggg cgagagacgg agaggcaagg gccacgatgg
     3901 tctgtaccag ggcttgagca ccgctactaa agacacttac gacgctctgc acatgcaggc
     3961 actgcctcca agagccacga acttctctct gttaaagcaa gcaggagatg ttgaagaaaa
     4021 ccccgggcct gctagcgaat tcgccaccat gtcgcccggg gccagccgcg gtccccgggg
     4081 aagccaggcg ccgctgatcg cgcccctctg ctgcgccgcg gccgcgctgg ggatgttgct
     4141 gtggtccccc gcctgtcagg cgttcaacct ggacgtggaa aagctcacag tgtacagcgg
     4201 ccccaagggc agctacttcg gctacgccgt ggacttccac atacccgacg cccgcacagc
     4261 gagtgtcttg gtgggggcgc ccaaagccaa caccagccag cccgatatcg tggaaggggg
     4321 agccgtctat tactgtcctt ggcccgcgga ggggtctgcg cagtgcaggc agataccgtt
     4381 tgacaccacc aacaacagaa agatcagagt taatggaacc aaagaaccta tcgagttcaa
     4441 atccaatcag tggtttggag caacagtgaa agctcacaaa ggaaaagttg tggcctgtgc
     4501 tcctttatat cactggagaa ctcttaaacc gacaccagaa aaggacccag ttggcacctg
     4561 ctatgtagca attcagaact tcagcgccta tgccgagttc tctccttgcc ggaacagcaa
     4621 tgctgatccg gaaggccagg gttactgcca agcaggattt agtctggatt tttataagaa
     4681 tggagacctt attgtgggag gacctgggag tttctactgg caaggacaag tgatcactgc
     4741 cagtgttgca gatatcattg caaattactc attcaaggat atcctcagga aactggcagg
     4801 agaaaagcag acggaagtgg ctccagcttc ctatgatgac agttaccttg gatactcagt
     4861 tgctgctggg gagtttactg gggattctca gcaagaattg gttgctggaa ttccaagagg
     4921 agcacagaat tttggatatg tttccatcat taactctacg gatatgacgt ttattcagaa
     4981 tttcacggga gaacagatgg catcttattt tggatatacc gttgtcgtat cagatgttaa
     5041 cagtgatgga ctggatgatg tcctggttgg ggcacctctc tttatggaac gtgaatttga
     5101 gagcaacccc agagaagtag ggcaaatcta cctgtatttg caagtgagct ctctcctctt
     5161 cagagacccc cagatcctca ctggcaccga gacgtttggg agattcggta gtgctatggc
     5221 acacttagga gacctgaacc aagatggata caatgacatt gccatcggag tgccttttgc
     5281 aggcaaggat caaagaggca aagtgctcat ttataatggg aacaaagatg gcttaaacac
     5341 caagccttcc caagttctgc aaggagtgtg ggcctcacat gctgtccctt ccggatttgg
     5401 ctttacttta agaggagatt cagacataga caagaatgat tacccagatt tgattgtggg
     5461 tgcatttgga acaggaaaag tcgctgttta cagagcaaga ccggttgtga ctgtagatgc
     5521 ccagcttctg ctgcacccaa tgattatcaa tcttgaaaat aaaacttgcc aggttccaga
     5581 ctctatgaca tctgctgcct gcttttcttt aagagtatgt gcatctgtca caggccagag
     5641 cattgcaaac acaatagtct tgatggcaga ggtgcaatta gattccctga aacagaaagg
     5701 agctattaaa cggacgctct tccttgataa ccatcaggct catcgcgtct tccctcttgt
     5761 gataaaaagg cagaaatccc accagtgcca ggatttcatc gtttaccttc gagatgaaac
     5821 tgaattccga gataaattat ctccaatcaa cattagtttg aattacagtt tggacgaatc
     5881 cacctttaaa gaaggcctgg aagtgaaacc aatattgaac tactacagag aaaacattgt
     5941 tagtgaacag gctcacattc tggtggactg tggagaagac aatctgtgtg ttcctgactt
     6001 gaagctgtcg gctagaccag ataagcatca ggtaatcatt ggagatgaaa atcaccttat
     6061 gctcataata aatgcaagaa atgaagggga aggagcatat gaagctgaac tctttgtaat
     6121 gataccagaa gaggcagatt atgttggaat cgaacgcaac aacaagggat ttcgaccact
     6181 gagctgtgag tacaagatgg aaaatgtaac caggatggtg gtgtgtgacc ttgggaaccc
     6241 tatggtgtct ggaacaaatt attccctggg cctccgattt gcagttccac gtcttgagaa
     6301 aacaaacatg agcattaact tcgatctcca aatcagaagt tccaacaagg acaatccaga
     6361 cagcaatttt gtgagcctgc aaatcaacat cactgctgta gcgcaggtgg aaataagagg
     6421 agtgtcacac cctccgcaga ttgttctgcc cattcataac tgggaaccag aagaggagcc
     6481 ccacaaagag gaggaggttg gaccattggt ggaacatatt tatgagctgc acaatattgg
     6541 accaagtacc atcagtgaca ccatcctgga ggtgggctgg cctttctctg cccgggatga
     6601 atttcttctc tatattttcc atattcaaac tctgggacct ctgcagtgcc aaccaaatcc
     6661 taatatcaat ccacaggata taaagcctgc tgcctcccca gaggacaccc ctgagctcag
     6721 cgcctttttg cgaaactcta ctattcctca tcttgtcagg aagagggatg tacatgtggt
     6781 cgaattccac agacagagcc ctgcaaaaat actgaattgt acaaatatcg agtgtttaca
     6841 aatctcctgt gcagtgggac gactcgaagg aggagaaagc gcagtcctga aagtcaggtc
     6901 acgattatgg gcccacacct tcctccagag aaaaaatgat ccctatgctc ttgcatccct
     6961 ggtgtccttt gaagttaaga agatgcctta tacagatcag ccagcaaaac tcccagaagg
     7021 aagcatagta attaagacat cagttatttg ggcaactccg aatgtttcct tctcaatccc
     7081 attatgggta ataatactag caatacttct tggattgttg gttctcgcca ttttaacctt
     7141 agctttatgg aagtgtggat tctttgacag agccagacct cctcaggagg acatgaccga
     7201 cagggaacag ctgacaaatg acaagacccc tgaggcatga gcggccgctg ggtaggggag
     7261 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     7321 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     7381 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     7441 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     7501 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     7561 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     7621 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     7681 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     7741 ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg gtgcgcctcg
     7801 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     7861 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     7921 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     7981 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     8041 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     8101 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     8161 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     8221 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     8281 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     8341 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt
     8401 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     8461 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     8521 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     8581 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     8641 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     8701 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     8761 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     8821 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     8881 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     8941 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     9001 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     9061 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     9121 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     9181 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9241 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     9301 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     9361 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9421 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     9481 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9541 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9601 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     9661 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     9721 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     9781 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     9841 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     9901 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     9961 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10021 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10081 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10141 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10201 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10261 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10321 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10381 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10441 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    10501 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10561 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10621 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10681 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10741 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10801 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    10861 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    10921 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    10981 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11041 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11101 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11161 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11221 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11281 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11341 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11401 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11461 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11521 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11581 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11641 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11701 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11761 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    11821 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    11881 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    11941 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    12001 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    12061 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    12121 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    12181 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    12241 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
