LOCUS       V044077                13124 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V044077
VERSION     V044077
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        1960..2171
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             2184..2452
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             2543..2566
                     /codon_start=1
                     /product="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
                     /transl_table=1
                     /translation="DYKDDDDK"
                     /label="FLAG"
     CDS             3974..4030
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4049..7237
                     /label="ITGA8(NM_003638)"
                     /note="ITGA8(NM_003638)"
                     /gene="ITGA8"
     CDS             7244..7333
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /transl_table=1
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
                     /label="3xMyc"
     CDS             7364..8083
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK*"
                     /label="EGFP"
     promoter        8093..8592
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             8613..9212
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    9219..9807
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             9881..10114
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    10186..10307
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      10326..10461
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(10499..10515)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    10523..10539
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(10547..10577)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10592..10613
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10901..11489)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11660..12520)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(12521..12625)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     13099..13115
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
     1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc
     2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
     2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct
     2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc
     2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc
     2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt
     2461 gaccggcgcc tactctagaa tggctctgcc agtgacagct ctgctgcttc cactggcact
     2521 gttgctgcat gccgcaaggc ctgactacaa agatgacgac gacaagggag gtggcggatc
     2581 tgagatcgtc ttgacacagt cacctgccac cttgtctctg tcacctggcg agagagccac
     2641 cttgagctgt agagccagcc aagacatcag caagtatctg aactggtatc agcagaagcc
     2701 aggtcaggca cctagactgc tgatctacca cacctctcgg cttcactccg gcattccaga
     2761 tcggttctct ggttccggca gcggcacaga cttcactctg accatcagca ggctggagcc
     2821 agaggacatc gccgtgtact actgtcaaca gggcaacaca ctgccataca ccttcggtca
     2881 gggaaccaag ctggaaatcg gtggcggcgg aagcggcgga ggtggatctg gaggcggtgg
     2941 aagccaagtc acattgagag aaagcggtcc agctctggtg aagcctactc agaccttgac
     3001 cttgacatgc accttctccg gcgttagcct gcctgactat ggagtgagct ggatcagaca
     3061 gccacctggt aaggctctgg aatggctcgc cgtgatctgg ggcagcgaga caacctacta
     3121 caacagcgca ctgaagtcca gactgaccat ctccaaagac acctccaaga accaggtggt
     3181 gttgaccatg acaaacatgg acccagtgga caccgctacc tactattgtg ccaagcacta
     3241 ctactacggt ggcagttacg ccatggatta ctggggtcag ggcaccactg tcaccgtgtc
     3301 cagcaccacc actccagcac ctaggcctcc aaccccagct ccaacaatcg ccagtcagcc
     3361 actgtctctt agacctgagg cctgcagacc agcagcaggt ggtgccgtgc ataccagagg
     3421 cttggacttc gcctgtgaca tctacatctg ggctccattg gctggcacat gcggcgtact
     3481 gctgctgagc ctggtcatca cactgtactg caagcgcggt cggaagaagt tgctgtacat
     3541 cttcaagcag ccatttatgc ggcctgtcca gaccacacaa gaagaagatg gatgcagctg
     3601 cagatttcca gaagaagaag agggcggttg tgagttgcgg gtcaagttct ccagatctgc
     3661 tgacgctcca gcttaccagc agggccagaa tcagctgtat aacgagttga acctgggcag
     3721 aagggaagaa tacgatgtgc tggataagag gcgcggaagg gaccctgaga tgggtggtaa
     3781 gcctagacgg aagaatccac aagagggtct gtacaatgag ctgcagaaag ataagatggc
     3841 cgaggcctac tccgagatcg gcatgaaggg cgagagacgg agaggcaagg gccacgatgg
     3901 tctgtaccag ggcttgagca ccgctactaa agacacttac gacgctctgc acatgcaggc
     3961 actgcctcca agagccacga acttctctct gttaaagcaa gcaggagatg ttgaagaaaa
     4021 ccccgggcct gctagcgaat tcgccaccat gtcgcccggg gccagccgcg gtccccgggg
     4081 aagccaggcg ccgctgatcg cgcccctctg ctgcgccgcg gccgcgctgg ggatgttgct
     4141 gtggtccccc gcctgtcagg cgttcaacct ggacgtggaa aagctcacag tgtacagcgg
     4201 ccccaagggc agctacttcg gctacgccgt ggacttccac atacccgacg cccgcacagc
     4261 gagtgtcttg gtgggggcgc ccaaagccaa caccagccag cccgatatcg tggaaggggg
     4321 agccgtctat tactgtcctt ggcccgcgga ggggtctgcg cagtgcaggc agataccgtt
     4381 tgacaccacc aacaacagaa agatcagagt taatggaacc aaagaaccta tcgagttcaa
     4441 atccaatcag tggtttggag caacagtgaa agctcacaaa ggaaaagttg tggcctgtgc
     4501 tcctttatat cactggagaa ctcttaaacc gacaccagaa aaggacccag ttggcacctg
     4561 ctatgtagca attcagaact tcagcgccta tgccgagttc tctccttgcc ggaacagcaa
     4621 tgctgatccg gaaggccagg gttactgcca agcaggattt agtctggatt tttataagaa
     4681 tggagacctt attgtgggag gacctgggag tttctactgg caaggacaag tgatcactgc
     4741 cagtgttgca gatatcattg caaattactc attcaaggat atcctcagga aactggcagg
     4801 agaaaagcag acggaagtgg ctccagcttc ctatgatgac agttaccttg gatactcagt
     4861 tgctgctggg gagtttactg gggattctca gcaagaattg gttgctggaa ttccaagagg
     4921 agcacagaat tttggatatg tttccatcat taactctacg gatatgacgt ttattcagaa
     4981 tttcacggga gaacagatgg catcttattt tggatatacc gttgtcgtat cagatgttaa
     5041 cagtgatgga ctggatgatg tcctggttgg ggcacctctc tttatggaac gtgaatttga
     5101 gagcaacccc agagaagtag ggcaaatcta cctgtatttg caagtgagct ctctcctctt
     5161 cagagacccc cagatcctca ctggcaccga gacgtttggg agattcggta gtgctatggc
     5221 acacttagga gacctgaacc aagatggata caatgacatt gccatcggag tgccttttgc
     5281 aggcaaggat caaagaggca aagtgctcat ttataatggg aacaaagatg gcttaaacac
     5341 caagccttcc caagttctgc aaggagtgtg ggcctcacat gctgtccctt ccggatttgg
     5401 ctttacttta agaggagatt cagacataga caagaatgat tacccagatt tgattgtggg
     5461 tgcatttgga acaggaaaag tcgctgttta cagagcaaga ccggttgtga ctgtagatgc
     5521 ccagcttctg ctgcacccaa tgattatcaa tcttgaaaat aaaacttgcc aggttccaga
     5581 ctctatgaca tctgctgcct gcttttcttt aagagtatgt gcatctgtca caggccagag
     5641 cattgcaaac acaatagtct tgatggcaga ggtgcaatta gattccctga aacagaaagg
     5701 agctattaaa cggacgctct tccttgataa ccatcaggct catcgcgtct tccctcttgt
     5761 gataaaaagg cagaaatccc accagtgcca ggatttcatc gtttaccttc gagatgaaac
     5821 tgaattccga gataaattat ctccaatcaa cattagtttg aattacagtt tggacgaatc
     5881 cacctttaaa gaaggcctgg aagtgaaacc aatattgaac tactacagag aaaacattgt
     5941 tagtgaacag gctcacattc tggtggactg tggagaagac aatctgtgtg ttcctgactt
     6001 gaagctgtcg gctagaccag ataagcatca ggtaatcatt ggagatgaaa atcaccttat
     6061 gctcataata aatgcaagaa atgaagggga aggagcatat gaagctgaac tctttgtaat
     6121 gataccagaa gaggcagatt atgttggaat cgaacgcaac aacaagggat ttcgaccact
     6181 gagctgtgag tacaagatgg aaaatgtaac caggatggtg gtgtgtgacc ttgggaaccc
     6241 tatggtgtct ggaacaaatt attccctggg cctccgattt gcagttccac gtcttgagaa
     6301 aacaaacatg agcattaact tcgatctcca aatcagaagt tccaacaagg acaatccaga
     6361 cagcaatttt gtgagcctgc aaatcaacat cactgctgta gcgcaggtgg aaataagagg
     6421 agtgtcacac cctccgcaga ttgttctgcc cattcataac tgggaaccag aagaggagcc
     6481 ccacaaagag gaggaggttg gaccattggt ggaacatatt tatgagctgc acaatattgg
     6541 accaagtacc atcagtgaca ccatcctgga ggtgggctgg cctttctctg cccgggatga
     6601 atttcttctc tatattttcc atattcaaac tctgggacct ctgcagtgcc aaccaaatcc
     6661 taatatcaat ccacaggata taaagcctgc tgcctcccca gaggacaccc ctgagctcag
     6721 cgcctttttg cgaaactcta ctattcctca tcttgtcagg aagagggatg tacatgtggt
     6781 cgaattccac agacagagcc ctgcaaaaat actgaattgt acaaatatcg agtgtttaca
     6841 aatctcctgt gcagtgggac gactcgaagg aggagaaagc gcagtcctga aagtcaggtc
     6901 acgattatgg gcccacacct tcctccagag aaaaaatgat ccctatgctc ttgcatccct
     6961 ggtgtccttt gaagttaaga agatgcctta tacagatcag ccagcaaaac tcccagaagg
     7021 aagcatagta attaagacat cagttatttg ggcaactccg aatgtttcct tctcaatccc
     7081 attatgggta ataatactag caatacttct tggattgttg gttctcgcca ttttaacctt
     7141 agctttatgg aagtgtggat tctttgacag agccagacct cctcaggagg acatgaccga
     7201 cagggaacag ctgacaaatg acaagacccc tgaggcactc gaggaacaaa aactaatatc
     7261 agaggaagat ttggaacaga aactaatcag tgaagaagat ttagagcaaa agttaatttc
     7321 cgaggaggac ttaggcggtg gaggaagcgg cggaggtgga tctatggtga gcaagggcga
     7381 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     7441 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     7501 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     7561 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     7621 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     7681 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     7741 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     7801 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     7861 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     7921 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     7981 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     8041 cgccgccggg atcactctcg gcatggacga gctgtacaag taggcggccg ctgggtaggg
     8101 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     8161 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     8221 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     8281 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     8341 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     8401 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     8461 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     8521 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     8581 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc
     8641 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     8701 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     8761 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     8821 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     8881 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     8941 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     9001 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     9061 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     9121 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     9181 cctggtgcat gacccgcaag cccggtgcct gagtcgacaa tcaacctctg gattacaaaa
     9241 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     9301 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     9361 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     9421 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9481 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9541 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9601 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     9661 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9721 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9781 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     9841 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     9901 caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     9961 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10021 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10081 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
    10141 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
    10201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
    10321 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
    10381 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
    10441 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt
    10501 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    10561 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    10621 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    10681 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    10741 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10801 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10861 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10921 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10981 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    11041 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    11101 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    11161 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    11221 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    11281 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    11341 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    11401 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    11461 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    11521 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    11581 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    11641 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    11701 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    11761 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    11821 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    11881 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    11941 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    12001 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    12061 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    12121 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    12181 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    12241 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    12301 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    12361 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    12421 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    12481 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    12541 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    12601 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
    12661 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    12721 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    12781 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    12841 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    12901 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
    12961 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    13021 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    13081 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
