LOCUS V039615 9605 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039615 VERSION V039615 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers LTR 234..414 /note="truncated 5' long terminal repeat (LTR) from HIV-1" /label="5' LTR (truncated)" misc_feature 458..583 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" misc_feature 1076..1309 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" CDS 1493..1537 /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 1798..1915 /note="central polypurine tract and central termination sequence of HIV-1" /label="cPPT/CTS" promoter 1960..2171 /note="core promoter for human elongation factor EF-1α" /label="EF-1α core promoter" LTR 2184..2452 /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" /label="5' LTR (truncated)" CDS 2498..3808 /label="CD177(NM_020406)" /note="CD177(NM_020406)" /gene="CD177" CDS 3845..4564 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" promoter 4574..5073 /note="mouse phosphoglycerate kinase 1 promoter" /label="PGK promoter" CDS 5094..5693 /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" misc_feature 5700..6288 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" LTR 6362..6595 /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" /label="3' LTR (ΔU3)" polyA_signal 6667..6788 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin 6807..6942 /note="SV40 origin of replication" /label="SV40 ori" primer_bind complement(6980..6996) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7004..7020 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7028..7058) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7073..7094 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7382..7970) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8141..9001) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9002..9106) /gene="bla" /label="AmpR promoter" primer_bind 9580..9596 /note="common sequencing primer, one of multiple similar variants" /label="M13 fwd" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg agcgcggtat tactgctggc 2521 cctcctgggg ttcatcctcc cactgccagg agtgcaggcg ctgctctgcc agtttgggac 2581 agttcagcat gtgtggaagg tgtccgacct gccccggcaa tggaccccta agaacaccag 2641 ctgcgacagc ggcttggggt gccaggacac gttgatgctc attgagagcg gaccccaagt 2701 gagcctggtg ctctccaagg gctgcacgga ggccaaggac caggagcccc gcgtcactga 2761 gcaccggatg ggccccggcc tctccctgat ctcctacacc ttcgtgtgcc gccaggagga 2821 cttctgcaac aacctcgtta actccctccc gctttgggcc ccacagcccc cagcagaccc 2881 aggatccttg aggtgcccag tctgcttgtc tatggaaggc tgtctggagg ggacaacaga 2941 agagatctgc cccaagggga ccacacactg ttatgatggc ctcctcaggc tcaggggagg 3001 aggcatcttc tccaatctga gagtccaggg atgcatgccc cagccagttt gcaacctgct 3061 caatgggaca caggaaattg ggcccgtggg tatgactgag aactgcgata tgaaagattt 3121 tctgacctgt catcggggga ccaccattat gacacacgga aacttggctc aagaacccac 3181 tgattggacc acatcgaata ccgagatgtg cgaggtgggg caggtgtgtc aggagacgct 3241 gctgctccta gatgtaggac tcacatcaac cctggtgggg acaaaaggct gcagcactgt 3301 tggggctcaa aattcccaga agaccaccat ccactcagcc cctcctgggg tgcttgtggc 3361 ctcctatacc cacttctgct cctcggacct gtgcaatagt gccagcagca gcagcgttct 3421 gctgaactcc ctccctcctc aagctgcccc tgtcccagga gaccggcagt gtcctacctg 3481 tgtgcagccc cttggaacct gttcaagtgg ctccccccga atgacctgcc ccaggggcgc 3541 cactcattgt tatgatgggt acattcatct ctcaggaggt gggctgtcca ccaaaatgag 3601 cattcagggc tgcgtggccc aaccttccag cttcttgttg aaccacacca gacaaatcgg 3661 gatcttctct gcgcgtgaga agcgtgatgt gcagcctcct gcctctcagc atgagggagg 3721 tggggctgag ggcctggagt ctctcacttg gggggtgggg ctggcactgg ccccagcgct 3781 gtggtgggga gtggtttgcc cttcctgcgg atccggcggt ggaggaagcg gcggaggtgg 3841 atctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 3901 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 3961 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 4021 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat 4081 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 4141 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 4201 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 4261 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 4321 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 4381 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 4441 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 4501 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 4561 gtaggcggcc gctgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta 4621 gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca 4681 tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact 4741 cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa 4801 atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag 4861 cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag 4921 aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc 4981 gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg 5041 ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg 5101 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 5161 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 5221 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 5281 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 5341 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 5401 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 5461 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 5521 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 5581 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 5641 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgagtcgaca 5701 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 5761 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 5821 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 5881 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5941 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 6001 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 6061 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 6121 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 6181 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 6241 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta 6301 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga 6361 ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg tactgggtct 6421 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 6481 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 6541 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 6601 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 6661 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 6721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 6781 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt 6841 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 6901 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt tgcagagacg 6961 gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 7021 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 7081 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 7141 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 7201 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 7261 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 7321 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 7381 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7441 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7501 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7561 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7621 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7681 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7741 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7801 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 7861 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7921 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7981 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 8041 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 8101 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 8161 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 8221 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 8281 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 8341 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 8401 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8461 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8521 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8581 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8641 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8701 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8761 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8821 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8881 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8941 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 9001 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 9061 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 9121 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 9181 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 9241 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 9301 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 9361 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 9421 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 9481 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 9541 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca 9601 agctg //