LOCUS       V027657                10116 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V027657
VERSION     V027657
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1494..1538
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1801..1916
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
                     /label="cPPT/CTS"
     enhancer        1974..2277
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2278..2481
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2588..4186
                     /label="Phgdh(NM_016966)"
                     /note="Phgdh(NM_016966)"
                     /gene="Phgdh"
     CDS             4211..4282
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags"
                     /transl_table=1
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
                     /label="3xFLAG"
     promoter        4336..4547
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4560..4828
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4856..5548
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="Zoanthus green fluorescent protein"
                     /transl_table=1
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
                     /label="ZsGreen1"
     CDS             5558..5611
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5612..6211
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6212..6799
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6873..7106
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7178..7299
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7318..7453
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7491..7507)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7515..7531
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7539..7569)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7584..7605
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7893..8481)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8652..9512)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9513..9617)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10091..10107
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     1801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     1861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
     1921 gtttattaca gggacagcag agatccagtt tatcgataag cttgggagtt ccgcgttaca
     1981 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2041 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     2101 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     2161 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     2221 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     2281 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     2341 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     2401 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     2461 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     2521 cgctgttttg acctccatag aagacaccga ctctactaga ggatctattt ccggtgaatt
     2581 cgccaccatg gccttcgcaa atctgcgcaa agtactcatc agtgacagcc tggacccctg
     2641 ctgccggaag atcctgcaag atggagggct gcaggtggtg gagaagcaga acttgagcaa
     2701 ggaggagctg atagctgaac tccaggactg tgaaggcctc attgtccggt ctgctactaa
     2761 ggtcactgct gatgtcatca atgcagcaga gaagctccag gtggtgggca gggctggcac
     2821 aggtgtggac aatgtggatc tggaggctgc cacgaggaag ggcatcctag tcatgaacac
     2881 ccccaacggg aacagcctca gtgctgcgga gctcacctgt gggatgatca tgtgcctggc
     2941 caggcagatt ccccaggcaa cagcttcgat gaaagatggc aaatgggacc ggaagaagtt
     3001 catggggaca gaactgaacg ggaagacact gggaattctt ggcctcggca gaattggaag
     3061 agaggtggcc acccgaatgc aatcctttgg aatgaagact gtaggctatg accccatcat
     3121 ctctcctgaa gtcgccgcct cctttggtgt tcagcagctg ccgctggagg agatctggcc
     3181 tctctgtgac tttataactg tccatacccc actcctgccc tctaccacag gcttgctgaa
     3241 tgacagcacc tttgctcagt gcaagaaagg tgtgcgagta gtgaactgtg ctcgaggagg
     3301 cattgtggat gaaggtgccc tgctgcgtgc cctgcagtct ggtcagtgtg ctggggctgc
     3361 actggatgtg tttacagaag agccaccacg ggaccgggcc ttagtggacc acgagaatgt
     3421 catcagctgt ccccacctgg gtgccagcac caaggaagcc cagagccgct gtggggagga
     3481 aatcgcagtc cagtttgtgg acatggtgaa ggggaaatct ctaacaggcg ttgtgaacgc
     3541 ccaggccctc accagtgcct tctctccaca caccaagcct tggattggtc tggcagaagc
     3601 aatgggcacg ctgatgcacg cctgggctgg ctcccctaaa gggaccatcc aggtggttac
     3661 acaaggaaca tctctgaaga atgctgggac ctgcctgagc cctgcagtca ttgtcggcct
     3721 tctgagagaa gcatctaagc aggcagacgt gaacttggtg aacgctaagc tactggtgaa
     3781 agaggctggc ctcaatgtca ccacctccca caaccctggg gttccagggg agcagggtag
     3841 cggggaatgc ctcctgactg tggccctggc aggtgctccc taccaagccg tgggcttggt
     3901 ccagggcacc acaccaatgc tgcagatgct caacggagct gtcttcagac cagaggtgcc
     3961 actacgcagg ggccaacccc tgctcgtatt ccgggctcag ccctccgacc ctgggatgct
     4021 gcccactatg attggcctcc tggcagaggc gggtgtacag ctgctgtcct accaaacctc
     4081 catggtgtct gacggagagc cctggcacgt catgggcctg tcctccctgc tgcccagcct
     4141 ggaaacatgg aagcagcatg tattggaggc tttccagttc tgcttcggat cctcggtacc
     4201 aagcttaagt gactacaagg atgacgatga caaggattac aaagacgacg atgataagga
     4261 ctataaggat gatgacgaca aataaagatc catcgatact agtaaggatc tgcgatcgct
     4321 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     4381 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     4441 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     4501 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag
     4561 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt
     4621 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg
     4681 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta
     4741 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt
     4801 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctact ctagcatggc
     4861 ccagtccaag cacggcctga ccaaggagat gaccatgaag taccgcatgg agggctgcgt
     4921 ggacggccac aagttcgtga tcaccggcga gggcatcggc taccccttca agggcaagca
     4981 ggccatcaac ctgtgcgtgg tggagggcgg ccccttgccc ttcgccgagg acatcttgtc
     5041 cgccgccttc atgtacggca accgcgtgtt caccgagtac ccccaggaca tcgtcgacta
     5101 cttcaagaac tcctgccccg ccggctacac ctgggaccgc tccttcctgt tcgaggacgg
     5161 cgccgtgtgc atctgcaacg ccgacatcac cgtgagcgtg gaggagaact gcatgtacca
     5221 cgagtccaag ttctacggcg tgaacttccc cgccgacggc cccgtgatga agaagatgac
     5281 cgacaactgg gagccctcct gcgagaagat catccccgtg cccaagcagg gcatcttgaa
     5341 gggcgacgtg agcatgtacc tgctgctgaa ggacggtggc cgcttgcgct gccagttcga
     5401 caccgtgtac aaggccaagt ccgtgccccg caagatgccc gactggcact tcatccagca
     5461 caagctgacc cgcgaggacc gcagcgacgc caagaaccag aagtggcacc tgaccgagca
     5521 cgccatcgcc tccggctccg ccttgcccgc ggccgctgag ggcagaggaa gtcttctaac
     5581 atgcggtgac gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct
     5641 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5701 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5761 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     5821 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     5881 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     5941 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6001 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6061 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6121 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6181 ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga
     6241 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6301 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6361 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6421 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6481 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6541 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6601 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6661 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6721 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc
     6781 cctttggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     6841 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa
     6901 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6961 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7021 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7081 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7141 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     7201 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7261 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     7321 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7381 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7441 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg
     7501 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7561 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7621 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7681 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7741 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7801 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7861 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7921 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7981 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8041 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8101 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8161 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8221 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8281 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     8341 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8401 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8461 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8521 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8581 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8641 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8701 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8761 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8821 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8881 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8941 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9001 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9061 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9181 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9241 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9301 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9361 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9421 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9481 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9541 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9601 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9661 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     9721 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     9781 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9841 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9901 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     9961 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    10021 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    10081 tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//
