LOCUS       V045702                10841 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045702
VERSION     V045702
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1798..1915
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2005..2208
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2297..5527
                     /label="RAPGEF1(NM_005312)"
                     /note="RAPGEF1(NM_005312)"
                     /gene="RAPGEF1"
     CDS             5528..5593
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        5644..5855
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5868..6136
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      6165..6174
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             6195..6857
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     misc_feature    6936..7524
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7598..7831
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7903..8024
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8043..8178
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8216..8232)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8240..8256
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8264..8294)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8309..8330
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8618..9206)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9377..10237)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10238..10342)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10816..10832
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gccaccatgg acacagactc tcagcgttct catctctctt ccttcaccat
     2341 gaagctgatg gacaaattcc actcacccaa aatcaagaga acgccatcaa agaagggaaa
     2401 accagctgag gtgtccgtaa agattccaga gaagcctgtg aacaaagagg caacagacag
     2461 atttctacca gagggctacc ctctcccctt ggatctggag cagcaggcag tagaatttat
     2521 gtccaccagt gctgtggctt ccaggtctca aaggcagaag aacctgagct ggctggagga
     2581 gaaagagaag gaagttgtca gtgccctgcg ctactttaag accattgtgg acaaaatggc
     2641 aattgataag aaggtactgg agatgcttcc agggtcagcc agcaaggtgc tggaggccat
     2701 cttacccctg gtgcagaacg atcctcgaat tcagcacagc tcagccctct cttcctgcta
     2761 tagccgagtg taccaaagcc tcgccaacct cattcgctgg tctgaccaag tgatgctgga
     2821 aggcgtgaac tcagaagaca aggagatggt gacgactgtg aagggggtca tcaaggctgt
     2881 gctggatgga gtgaaggagc tggtcaggct caccatcgag aagcagggac gtccgtctcc
     2941 gacgagcccc gtgaagccca gttcccctgc cagcaagcct gatggcccag cagagctccc
     3001 cctgacagac cgcgaggtag agatcctaaa caagacgact gggatgtcac agtcaactga
     3061 gctcctccca gatgccacgg atgaagaggt cgcgcccccc aagcctcctc tgcctggcat
     3121 tcgggtggtt gataatagtc ctccaccagc attgccaccc aagaaaagac agtcggcgcc
     3181 gtcccctacc cgagtggctg tggtggcccc catgagccga gccaccagtg gctccagttt
     3241 gcctgttgga atcaataggc aggattttga tgttgactgt tacgcacaga ggcgactgtc
     3301 aggaggcagc cactcatatg gtggagagtc gccccgcctc tccccctgca gcagcatagg
     3361 caagctcagc aagtcagacg agcagctgtc ctctctggac agggacagtg ggcagtgctc
     3421 ccggaacaca agctgtgaaa cactagacca ctatgatccc gactatgaat tcctccagca
     3481 agacctctct aacgcagacc agatacctca gcagacggcc tggaacctta gcccgttgcc
     3541 agagtctttg ggggagtctg ggtctccatt tcttggccct cctttccagc tgcctcttgg
     3601 cggccatccc cagccagacg gacctctggc cccagggcag cagacagata cgccacctgc
     3661 tctccccgag aagaagcgca ggagcgcagc ctcccagacg gcggacggct ctggctgcag
     3721 ggtgtcctac gagcggcatc cctcgcagta tgacaacatc tctggggagg acctgcagag
     3781 cacagccccg atcccatccg tcccctacgc gccctttgct gctattctgc cctttcagca
     3841 tggaggttcc tcagcccctg tcgaatttgt gggtgatttt actgctcctg agtcaaccgg
     3901 tgacccagaa aaaccacctc ctctaccaga gaagaaaaac aaacacatgc tggcctacat
     3961 gcagttgctg gaggactact cggagccgca gccctctatg ttctaccaga cgccacagaa
     4021 cgagcacatc taccagcaga agaacaagct cctcatggag gtatacggct tcagcgactc
     4081 cttcagtggg gtggactccg tgcaggagct ggccccgccg cccgccctac cccccaagca
     4141 gcggcagctg gagccaccgg ctgggaaaga cggacatccc agagatccct cagcggtcag
     4201 cggcgtccct gggaaggaca gcagagacgg cagtgagagg gccccaaagt caccagatgc
     4261 tctggagtcg gctcagtcgg aggaggaagt ggacgagctg tccctcattg accacaacga
     4321 aattatgtcc aggctgacgc tcaagcagga gggtgatgac gggccggacg tccgcggagg
     4381 atctggggac atcttactgg tccatgctac tgagactgac aggaaagatt tggtgttgta
     4441 ctgcgaggca ttcctgacca cctacaggac cttcatctcc ccagaggagc tcatcaagaa
     4501 gctgcagtac agatatgaga aattctctcc ctttgccgac acattcaaga agcgcgtcag
     4561 caagaacacg ttcttcgtgc tggtacgggt ggtggatgag ctctgcctgg tggagttgac
     4621 agaagagatc ctgaagctgc tgatggaact ggtcttccgc ctggtgtgca atggggagct
     4681 gagcctggcc cgtgtgctcc ggaagaacat cctggacaag gtggaccaga agaagctact
     4741 caggtgtgcc acctccagcc agcccctggc agcccggggg gtagcagcca ggccggggac
     4801 cttgcacgac tttcacagcc atgagatagc ggagcagcta acgctgctgg atgctgagct
     4861 cttctataaa atagagattc ctgaggtttt gctttgggca aaagagcaga atgaggagaa
     4921 gagccccaac ttgacccagt tcacggagca cttcaacaac atgtcctact gggtccggtc
     4981 cataatcatg ttacaggaaa aggcccagga cagggaacgg ctgctcttga agttcatcaa
     5041 gatcatgaag cacttgcgga agctgaataa cttcaactcc tacttggcca tcctctctgc
     5101 cctggactcg gcgcccatcc gcaggctgga gtggcagaag cagacttcag agggcctggc
     5161 cgagtactgc acactgatcg acagctcgtc ctccttccga gcctaccggg ccgccctctc
     5221 ggaggtggaa ccgccgtgca tcccgtacct ggggctgatc ctgcaggacc tgaccttcgt
     5281 tcacctggga aacccagact acatcgacgg gaaagtgaac ttctccaagc ggtggcagca
     5341 gttcaacatc ctcgacagca tgcgctgctt ccagcaggcg cactatgaca tgcggaggaa
     5401 cgacgacatt ataaacttct tcaatgactt cagtgaccac ctggctgagg aggccctatg
     5461 ggaactgtct ctgaaaatta aacccaggaa cataacaagg agaaaaacag accgggaaga
     5521 gaagaccgac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
     5581 tgacgatgac aagtagggat ccgcggccgc gaaggatctg cgatcgctcc ggtgcccgtc
     5641 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     5701 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     5761 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     5821 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct
     5881 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc
     5941 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc
     6001 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc
     6061 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc
     6121 tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agacgccacc atggagagcg
     6181 acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc ctgaacggcg
     6241 tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc atgaccaaca
     6301 agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc cacgtgatgg
     6361 gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc ttcctgcacg
     6421 ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc
     6481 tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg
     6541 tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc cgcagcaacg
     6601 ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca
     6661 ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac atgcacttca
     6721 agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc ttccgccgcg
     6781 tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac gccttcaaga
     6841 cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg
     6901 ccggacccgg ctccaccgga tctcgctaag tcgacaatca acctctggat tacaaaattt
     6961 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7021 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7081 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7141 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7201 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7261 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7321 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     7381 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7441 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7501 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
     7561 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     7621 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     7681 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7741 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7801 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     7861 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     7921 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7981 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     8041 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8161 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
     8221 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8281 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8341 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8401 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8461 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8521 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8581 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9301 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9361 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9421 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9481 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9541 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9601 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9661 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9721 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9781 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9841 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9901 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9961 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10021 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10081 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10141 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10201 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10261 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10321 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10381 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    10441 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    10501 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    10561 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    10621 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    10681 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    10741 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
    10801 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
