LOCUS       V015197                 8563 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015197
VERSION     V015197
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1798..1915
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2005..2208
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        2348..2559
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             2572..2840
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             2924..3637
                     /label="GFP"
                     /note="green fluorescent protein"
     CDS             3701..4648
                     /label="iRFP720"
                     /note="near-infrared fluorescent protein with an emission
                     peak at 720 nm, engineered from a bacterial phytochrome
                     (Shcherbakova and Verkhusha, 2013)"
     misc_feature    4658..5246
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5320..5553
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5625..5759
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5765..5900
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(5938..5954)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5962..5978)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5986..6016)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6031..6052)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6340..6928)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7102..7959)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7960..8064)
                     /label="AmpR promoter"
     primer_bind     8538..8554
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc
     2281 tagcgaattc gaatttaaat ggatccgcgg ccgcgaagga tctgcgatcg ctccggtgcc
     2341 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     2401 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     2461 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     2521 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     2581 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     2641 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     2701 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     2761 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     2821 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagatga ccgagtacaa
     2881 gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gacatgagca agggcgagga
     2941 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     3001 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     3061 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     3121 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     3181 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     3241 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     3301 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     3361 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     3421 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     3481 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     3541 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     3601 cgccgggatc actcacggca tggacgagct gtacaagggt accgccacga acttctctct
     3661 gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct atggcggaag gatccgtcgc
     3721 caggcagcct gacctcttga cctgcgacga tgagccgatc catatccccg gtgccatcca
     3781 accgcatgga ctgctgctcg ccctcgccgc cgacatgacg atcgttgccg gcagcgacaa
     3841 ccttcccgaa ctcaccggac tggcgatcgg cgccctgatc ggccgctctg cggccgatgt
     3901 cttcgactcg gagacgcaca accgtctgac gatcgccttg gccgagcccg gggcggccgt
     3961 cggagcaccg atcactgtcg gcttcacgat gcgaaaggac gcaggcttca tcggctcctg
     4021 gcatcgccat gatcagctca tcttcctcga gctcgagcct ccccagcggg acgtcgccga
     4081 gccgcaggcg ttcttccgcc gcaccaacag cgccatccgc cgcctgcagg ccgccgaaac
     4141 cttggaaagc gcctgcgccg ccgcggcgca agaggtgcgg aagattaccg gcttcgatcg
     4201 ggtgatgatc tatcgcttcg cctccgactt cagcgggtcc gtgatcgcag aggatcggtg
     4261 cgccgaggtc gagtcaaaac taggcctgca ctatcctgcc tcattcatcc cggcgcaggc
     4321 ccgtcggctc tataccatca acccggtacg gatcattccc gatatcaatt atcggccggt
     4381 gccggtcacc ccagacctca atccggtcac cgggcggccg attgatctta gcttcgccat
     4441 cctgcgcagc gtctcgccca accatctgga gttcatgcgc aacataggca tgcacggcac
     4501 gatgtcgatc tcgattttgc gcggcgagcg actgtgggga ttgatcgttt gccatcaccg
     4561 aacgccgtac tacgtcgatc tcgatggccg ccaagcctgc gagctagtcg cccaggttct
     4621 ggcctggcag atcggcgtga tggaagagtg agtcgacaat caacctctgg attacaaaat
     4681 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     4741 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     4801 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     4861 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     4921 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     4981 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     5041 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     5101 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     5161 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     5221 gatctccctt tgggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag
     5281 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5341 aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5401 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5461 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5521 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5581 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5641 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5701 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5761 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5821 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5881 aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc
     5941 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6001 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6061 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6781 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7021 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7081 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7141 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7201 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7261 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7321 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7381 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7441 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7501 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7561 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7621 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7681 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7741 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7801 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7861 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7921 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     8101 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8161 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     8221 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8281 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8341 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     8401 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     8461 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     8521 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg
//
