LOCUS       V020598                11632 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020598
VERSION     V020598
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2294..5803
                     /label="THBS1(NM_003246)"
                     /note="THBS1(NM_003246)"
                     /gene="THBS1"
     promoter        5853..6064
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6077..6345
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6380..7072
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             7073..7126
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7127..7726
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7727..8315
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8389..8622
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8694..8815
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8834..8969
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9007..9023)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9031..9047
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9055..9085)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9100..9121
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9409..9997)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10168..11028)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11029..11133)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11607..11623
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcc accatggggc tggcctgggg actaggcgtc ctgttcctga tgcatgtgtg
     2341 tggcaccaac cgcattccag agtctggcgg agacaacagc gtgtttgaca tctttgaact
     2401 caccggggcc gcccgcaagg ggtctgggcg ccgactggtg aagggccccg acccttccag
     2461 cccagctttc cgcatcgagg atgccaacct gatcccccct gtgcctgatg acaagttcca
     2521 agacctggtg gatgctgtgc gggcagaaaa gggtttcctc cttctggcat ccctgaggca
     2581 gatgaagaag acccggggca cgctgctggc cctggagcgg aaagaccact ctggccaggt
     2641 cttcagcgtg gtgtccaatg gcaaggcggg caccctggac ctcagcctga ccgtccaagg
     2701 aaagcagcac gtggtgtctg tggaagaagc tctcctggca accggccagt ggaagagcat
     2761 caccctgttt gtgcaggaag acagggccca gctgtacatc gactgtgaaa agatggagaa
     2821 tgctgagttg gacgtcccca tccaaagcgt cttcaccaga gacctggcca gcatcgccag
     2881 actccgcatc gcaaaggggg gcgtcaatga caatttccag ggggtgctgc agaatgtgag
     2941 gtttgtcttt ggaaccacac cagaagacat cctcaggaac aaaggctgct ccagctctac
     3001 cagtgtcctc ctcacccttg acaacaacgt ggtgaatggt tccagccctg ccatccgcac
     3061 taactacatt ggccacaaga caaaggactt gcaagccatc tgcggcatct cctgtgatga
     3121 gctgtccagc atggtcctgg aactcagggg cctgcgcacc attgtgacca cgctgcagga
     3181 cagcatccgc aaagtgactg aagagaacaa agagttggcc aatgagctga ggcggcctcc
     3241 cctatgctat cacaacggag ttcagtacag aaataacgag gaatggactg ttgatagctg
     3301 cactgagtgt cactgtcaga actcagttac catctgcaaa aaggtgtcct gccccatcat
     3361 gccctgctcc gctgccacag ttcctgatgg agaatgctgt cctcgctgtt ggcccagcga
     3421 ctctgcggac gatggctggt ctccatggtc cgagtggacc tcctgttcta cgagctgtgg
     3481 caatggaatt cagcagcgcg gccgctcctg cgatagcctc aacaaccgat gtgagggctc
     3541 ctcggtccag acacggacct gccacattca ggagtgtgac aagagattta aacaggatgg
     3601 tggctggagc cactggtccc cgtggtcatc ttgttctgtg acatgtggtg atggtgtgat
     3661 cacaaggatc cggctctgca actctcccag cccccagatg aacgggaaac cctgtgaagg
     3721 cgaagcgcgg gagaccaaag cctgcaagaa agacgcctgc cccatcaatg gaggctgggg
     3781 tccttggtca ccatgggaca tctgttctgt cacctgtgga ggaggggtac agaaacgtag
     3841 tcgtctctgc aacaacccca caccccagtt tggaggcaag gactgcgttg gtgatgtaac
     3901 agaaaaccag atctgcaaca agcaggactg tccaattgat ggatgcctgt ccaatccctg
     3961 ctttgccggc gtgaagtgta ctagctaccc tgatggcagc tggaaatgtg gtgcttgtcc
     4021 ccctggttac agtggaaatg gcatccagtg cacagatgtt gatgagtgca aagaagtgcc
     4081 tgatgcctgc ttcaaccaca atggagagca ccggtgtgag aacacggacc ccggctacaa
     4141 ctgcctgccc tgccccccac gcttcaccgg ctcacagccc ttcggccagg gtgtcgaaca
     4201 tgccacggcc aacaaacagg tgtgcaagcc ccgtaacccc tgcacggatg ggacccacga
     4261 ctgcaacaag aacgccaagt gcaactacct gggccactat agcgacccca tgtaccgctg
     4321 cgagtgcaag cctggctacg ctggcaatgg catcatctgc ggggaggaca cagacctgga
     4381 tggctggccc aatgagaacc tggtgtgcgt ggccaatgcg acttaccact gcaaaaagga
     4441 taattgcccc aaccttccca actcagggca ggaagactat gacaaggatg gaattggtga
     4501 tgcctgtgat gatgacgatg acaatgataa aattccagat gacagggaca actgtccatt
     4561 ccattacaac ccagctcagt atgactatga cagagatgat gtgggagacc gctgtgacaa
     4621 ctgtccctac aaccacaacc cagatcaggc agacacagac aacaatgggg aaggagacgc
     4681 ctgtgctgca gacattgatg gagacggtat cctcaatgaa cgggacaact gccagtacgt
     4741 ctacaatgtg gaccagagag acactgatat ggatggggtt ggagatcagt gtgacaattg
     4801 ccccttggaa cacaatccgg atcagctgga ctctgactca gaccgcattg gagatacctg
     4861 tgacaacaat caggatattg atgaagatgg ccaccagaac aatctggaca actgtcccta
     4921 tgtgcccaat gccaaccagg ctgaccatga caaagatggc aagggagatg cctgtgacca
     4981 cgatgatgac aacgatggca ttcctgatga caaggacaac tgcagactcg tgcccaatcc
     5041 cgaccagaag gactctgacg gcgatggtcg aggtgatgcc tgcaaagatg attttgacca
     5101 tgacagtgtg ccagacatcg atgacatctg tcctgagaat gttgacatca gtgagaccga
     5161 tttccgccga ttccagatga ttcctctgga ccccaaaggg acatcccaaa atgaccctaa
     5221 ctgggttgta cgccatcagg gtaaagaact cgtccagact gtcaactgtg atcctggact
     5281 cgctgtaggt tatgatgagt ttaatgctgt ggacttcagt ggcaccttct tcatcaacac
     5341 cgaaagggac gatgactatg ctggatttgt ctttggctac cagtccagca gccgctttta
     5401 tgttgtgatg tggaagcaag tcacccagtc ctactgggac accaacccca cgagggctca
     5461 gggatactcg ggcctttctg tgaaagttgt aaactccacc acagggcctg gcgagcacct
     5521 gcggaacgcc ctgtggcaca caggaaacac ccctggccag gtgcgcaccc tgtggcatga
     5581 ccctcgtcac ataggctgga aagatttcac cgcctacaga tggcgtctca gccacaggcc
     5641 aaagacgggt ttcattagag tggtgatgta tgaagggaag aaaatcatgg ctgactcagg
     5701 acccatctat gataaaacct atgctggtgg tagactaggg ttgtttgtct tctctcaaga
     5761 aatggtgttc ttctctgacc tgaaatacga atgtagagat ccctaaggat ccgcggccgc
     5821 aaggatctgc gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     5881 cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt
     5941 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     6001 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     6061 acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg
     6121 ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac
     6181 tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct
     6241 cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca
     6301 actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc
     6361 gcctacgcta gacgccacca tgagcgagct gatcaaggag aacatgcaca tgaagctgta
     6421 catggagggc accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc
     6481 ctacgagggc acccagacca tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc
     6541 cttcgacatc ctggctacca gcttcatgta cggcagcaaa gccttcatca accacaccca
     6601 gggcatcccc gacttcttta agcagtcctt ccctgagggc ttcacatggg agagaatcac
     6661 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcttcc agaacggctg
     6721 catcatctac aacgtcaaga tcaacggggt gaacttccca tccaacggcc ctgtgatgca
     6781 gaagaaaaca cgcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct
     6841 gagaggccac agccagatgg ccctgaagct cgtgggcggg ggctacctgc actgctcctt
     6901 caagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcttccactt
     6961 cgtggaccac agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca
     7021 cgagatggct gtggccaagt actgcgacct ccctagcaaa ctggggcaca gagagggcag
     7081 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa
     7141 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     7201 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     7261 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     7321 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     7381 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     7441 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     7501 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     7561 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     7621 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     7681 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga
     7741 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7801 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7861 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7921 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7981 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8041 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8101 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     8161 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8221 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8281 gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt
     8341 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     8401 ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     8461 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     8521 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     8581 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     8641 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     8701 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8761 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8821 tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8881 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8941 gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta
     9001 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9061 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9121 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9181 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9241 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9301 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9361 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9421 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9481 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9541 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9601 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9661 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9721 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9781 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9841 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9901 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9961 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10021 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10081 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10141 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10201 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10261 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10321 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10381 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10441 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10501 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10561 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10621 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10681 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10741 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10801 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10861 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10921 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10981 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11041 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11101 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11161 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    11221 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    11281 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    11341 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    11401 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    11461 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    11521 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    11581 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
