LOCUS       V038801                10182 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038801
VERSION     V038801
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4266
                     /label="SMPD3(NM_018667)"
                     /note="SMPD3(NM_018667)"
                     /gene="SMPD3"
     CDS             4267..4293
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4297..4323
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4327..4353
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     promoter        4403..4614
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4627..4895
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4930..5622
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             5623..5676
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5677..6276
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6277..6865
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6939..7172
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7244..7365
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7384..7519
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7557..7573)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7581..7597
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7605..7635)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7650..7671
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7959..8547)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8718..9578)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9579..9683)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10157..10173
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggttttg tacacgaccc cctttcctaa cagctgtctg
     2341 tccgccctgc actgtgtgtc ctgggccctt atctttccat gctactggct ggtggaccgg
     2401 ctcgctgcct ccttcatacc caccacctac gagaagcgcc agcgggcaga cgacccgtgc
     2461 tgcctgcagc tgctctgcac tgccctcttc acgcccatct acctggccct cctggtggcc
     2521 tcgctgccct ttgcgtttct cggctttctc ttctggtccc cactgcagtc ggcccgccgg
     2581 ccctacatct attcacggct ggaagacaag ggcctggccg gtggggcagc cctgctcagt
     2641 gaatggaagg gcacggggcc tggcaaaagc ttctgctttg ccactgccaa cgtctgcctc
     2701 ctgcccgact cactcgccag ggtcaacaac ctttttaaca cccaagcgcg ggccaaggag
     2761 atcgggcaga gaatccgcaa tggggccgcc cggccccaga tcaaaattta catcgactcc
     2821 cccaccaata cctccatcag cgccgctagc ttcagcagcc tggtgtcacc acagggcggc
     2881 gatggggtgg cccgggccgt ccccgggagc attaagagga cagcctctgt ggagtacaag
     2941 ggtgacggtg ggcggcaccc cggtgacgag gctgccaacg gcccagcctc tggggaccct
     3001 gtcgacagca gcagcccgga ggatgcctgc atcgtgcgca tcggtggcga ggagggcggc
     3061 cggccacctg aagctgacga ccctgtgcct gggggccagg ccaggaacgg agctggcggg
     3121 ggcccaaggg gccagacgcc caaccataat cagcaggacg gggattcagg gagcctgggc
     3181 agcccctcgg cctcccggga gtccctggtg aaggggcgag ctgggccaga caccagtgcc
     3241 agcggggagc caggtgccaa cagcaagctc ctgtacaagg cctcggtggt gaagaaggcg
     3301 gctgcacgca ggaggcggca ccccgacgag gccttcgacc atgaggtctc cgccttcttc
     3361 cccgccaacc tggacttcct gtgcctgcag gaggtgtttg acaagcgagc agccaccaaa
     3421 ttgaaagagc agctgcacgg ctacttcgag tacatcctgt acgacgtcgg ggtctacggc
     3481 tgccagggct gctgcagctt caagtgtctc aacagcggcc tcctctttgc cagccgctac
     3541 cccatcatgg acgtggccta tcactgttac cccaacaagt gtaacgacga tgccctggcc
     3601 tctaagggag ctctgtttct caaggtgcag gtgggaagca cacctcagga ccaaagaatc
     3661 gtcgggtaca tcgcctgcac acacctgcat gccccgcaag aggacagcgc catccggtgt
     3721 gggcagctgg acctgcttca ggactggctg gctgatttcc gaaaatctac ctcctcgtcc
     3781 agcgcagcca accccgagga gctggtggca tttgacgtcg tctgtggaga tttcaacttt
     3841 gataactgct cctctgacga caagctggag cagcaacact ccctgttcac ccactacagg
     3901 gacccctgcc gcctggggcc tggtgaggag aagccgtggg ccatcggtac tctgctggac
     3961 acgaacggcc tgtacgatga ggatgtgtgc acccccgaca acctgcagaa ggtcctggag
     4021 agtgaggagg gccgcaggga gtacctggcg tttcccacca gcaagagctc gggccagaag
     4081 gggcggaagg agctgctgaa gggcaacggc cggcgcatcg actacatgct gcatgcagag
     4141 gaggggctgt gcccagactg gaaggccgag gtggaagaat tcagttttat cacccagctg
     4201 tccggcctga cggaccacct gccagtagcc atgcgactga tggtgtcttc gggggaggag
     4261 gaggcatacc catacgacgt acctgattat gcaacttatc cttatgatgt acctgattat
     4321 gctagctatc cttatgatgt cccagactac gcatagggat ccgcggccgc aaggatctgc
     4381 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     4441 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     4501 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     4561 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     4621 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     4681 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     4741 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     4801 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     4861 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta
     4921 gacgccacca tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc
     4981 accgtgaaca accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     5041 acccagacca tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     5101 ctggctacca gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc
     5161 gacttcttta agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa
     5221 gacgggggcg tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac
     5281 aacgtcaaga tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca
     5341 cgcggctggg aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac
     5401 agccagatgg ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca
     5461 tacagatcca agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac
     5521 agactggaaa gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct
     5581 gtggccaagt actgcgacct ccctagcaaa ctggggcaca gagagggcag aggaagtctt
     5641 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg
     5701 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     5761 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     5821 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     5881 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     5941 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     6001 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     6061 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     6121 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     6181 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     6241 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt
     6301 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6361 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6421 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6481 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6541 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6601 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6661 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6721 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6781 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6841 atctcccttt gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc
     6901 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6961 acgaaaataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     7021 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7081 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7141 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7201 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7261 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7321 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7381 gctatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7441 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7501 ggaggctttt ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca
     7561 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     7621 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     7681 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     7741 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     7801 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     7861 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     7921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     7981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     8401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8701 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     8761 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     8821 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     8881 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     8941 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9001 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9061 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9121 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9181 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9241 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9301 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9361 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9421 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9481 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9541 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9601 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9661 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     9721 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     9781 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     9841 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9901 ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     9961 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
    10021 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
    10081 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
    10141 tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tg
//
