LOCUS       V047216                10947 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V047216
VERSION     V047216
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5118
                     /label="MORC3(NM_015358)"
                     /note="MORC3(NM_015358)"
                     /gene="MORC3"
     promoter        5168..5379
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             5392..5660
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5695..6387
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             6388..6441
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6442..7041
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7042..7630
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7704..7937
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8009..8130
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8149..8284
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8322..8338)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8346..8362
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8370..8400)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8415..8436
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8724..9312)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9483..10343)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10344..10448)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10922..10938
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggcg cagccacccc gcgggatacg cctcagcgcg
     2341 ctttgcccga agtttttaca tacaaattct actagtcaca cctggccatt cagtgcagtt
     2401 gctgaattaa tagataatgc ttatgatcct gatgtgaacg ctaaacaaat atggattgac
     2461 aaaacagtga taaatgacca tatatgcttg acattcaccg acaatgggaa tggtatgact
     2521 tctgataaat tacataaaat gctaagcttt ggcttcagtg acaaagtcac catgaatggt
     2581 catgtcccag ttggattata tgggaatggc ttcaagtcgg gttctatgcg tctgggtaaa
     2641 gacgcaatcg tttttaccaa aaatggagaa agcatgagcg tgggcctttt gtctcagacc
     2701 tacttggaag tcataaaagc ggagcatgtt gttgttccaa tagtggcatt caacaagcac
     2761 cgacagatga ttaatttagc agaatcaaaa gccagccttg ctgcaattct ggaacattct
     2821 ctgttttcca cggaacagaa gttactggca gaacttgatg ctattatagg caagaagggg
     2881 acgaggatca tcatttggaa tcttagaagc tacaaaaatg caacagagtt cgattttgaa
     2941 aaggataaat atgatatcag aattcccgag gatttagatg agataacagg gaagaagggg
     3001 tacaagaagc aggaaaggat ggaccagatt gcccctgaga gtgactattc cctgagggct
     3061 tattgcagta tattatatct aaagccaaga atgcagatca tcctacgtgg acagaaagtg
     3121 aagacacagc tggtttcgaa gagtcttgcc tacatcgaac gtgatgttta tcgaccaaaa
     3181 tttttatcta aaacagtgag aattaccttt ggattcaact gcagaaataa agatcattat
     3241 gggataatga tgtatcacag aaatagactc atcaaagctt atgaaaaagt tggatgtcag
     3301 ttaagggcaa acaacatggg tgttggagtg gttggaatta tagagtgtaa tttccttaag
     3361 ccaactcata ataaacaaga tttcgactat actaatgagt acagacttac aataacagca
     3421 ctaggagaaa agctgaatga ttactggaat gaaatgaaag tgaagaaaaa tacagaatat
     3481 cctctaaatt tgccagttga agatatacag aagcgtcctg atcagacatg ggttcagtgt
     3541 gatgcctgtc taaagtggcg gaaattacct gatgggatgg atcaacttcc tgaaaaatgg
     3601 tattgctcca ataaccctga cccacagttc agaaattgtg aggttccaga agaacctgaa
     3661 gatgaggatt tggtacatcc cacttatgaa aaaacctaca aaaagaccaa caaggaaaaa
     3721 ttcaggatca gacaaccgga aatgatccct cggattaatg ctgaactgtt gtttcggcca
     3781 actgctcttt caactccaag cttttcttct cctaaggaaa gtgttccaag aagacatctt
     3841 tcagaaggaa caaattctta tgcgacaaga cttctaaata atcatcaagt tccacctcag
     3901 tctgaacctg agagcaacag cttgaaacgg agactttcta ctcgttcctc aattttgaat
     3961 gcaaagaatc ggagattgag tagtcagttt gaaaattcag tttataaagg tgatgatgat
     4021 gatgaagatg tcatcatctt agaagaaaac agtaccccca aacctgcagt agatcatgat
     4081 attgacatga aatcagaaca gagtcacgtt gagcaaggtg gtgttcaggt tgagtttgtg
     4141 ggtgacagtg aaccttgtgg ccagactggt tcaacaagca cctcatcatc ccgatgcgac
     4201 cagggaaata ctgcagctac ccagactgaa gtaccaagtt tagttgttaa aaaagaagaa
     4261 actgttgaag acgagataga cgtaagaaat gatgcagtga ttctgccctc ctgtgtagaa
     4321 gctgaagcaa agatacatga aacccaggaa accaccgata aatctgcaga tgatgcaggc
     4381 tgccaattac aagaactgag aaaccagcta ctccttgtca ctgaggaaaa agagaattat
     4441 aaaagacagt gtcatatgtt tactgatcaa atcaaagtgt tacaacagag gatactagaa
     4501 atgaatgaca agtatgttaa gaaagaaact tgccatcagt ccactgaaac cgatgctgta
     4561 tttttacttg aaagtattaa tggcaaatct gaaagtccag accatatggt atctcagtat
     4621 cagcaagctt tggaagaaat agaaaggctg aaaaaacaat gtagtgcttt gcaacatgta
     4681 aaggctgaat gcagccagtg ttccaataat gagagtaaaa gtgaaatgga tgagatggct
     4741 gtgcagcttg acgatgtgtt tagacaactg gacaaatgca gtattgagag ggaccagtat
     4801 aaaagtgagg ttgaattgct ggaaatggaa aagtcacaaa tccgttcaca gtgtgaagaa
     4861 ctcaaaactg aagtagaaca gttaaaatct acaaatcaac agacggcaac agatgtttca
     4921 acatcaagta acattgagga gtctgtaaat catatggatg gagaaagcct caaactccga
     4981 tctcttcgag ttaacgtagg acaactgctg gctatgattg tgcctgatct tgatcttcag
     5041 caagtgaatt acgatgttga tgtagttgat gagattttag gacaagttgt tgaacaaatg
     5101 agtgaaatca gtagtactta aggatccgcg gccgcaagga tctgcgatcg ctccggtgcc
     5161 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     5221 aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     5281 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     5341 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg aggggctcgc
     5401 atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg gttgagtcgc
     5461 gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta
     5521 aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc tagactcagc
     5581 cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt ttcgttttct
     5641 gttctgcgcc gttacagatc caagctgtga ccggcgccta cgctagacgc caccatgagc
     5701 gagctgatca aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac
     5761 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaag
     5821 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc
     5881 atgtacggca gcaaagcctt catcaaccac acccagggca tccccgactt ctttaagcag
     5941 tccttccctg agggcttcac atgggagaga atcaccacat acgaagacgg gggcgtgctg
     6001 accgctaccc aggacaccag cttccagaac ggctgcatca tctacaacgt caagatcaac
     6061 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacacgcgg ctgggaggcc
     6121 aacaccgaga tgctgtaccc cgctgacggc ggcctgagag gccacagcca gatggccctg
     6181 aagctcgtgg gcgggggcta cctgcactgc tccttcaaga ccacatacag atccaagaaa
     6241 cccgctaaga acctcaagat gcccggcttc cacttcgtgg accacagact ggaaagaatc
     6301 aaggaggccg acaaagagac ctacgtcgag cagcacgaga tggctgtggc caagtactgc
     6361 gacctcccta gcaaactggg gcacagagag ggcagaggaa gtcttctaac atgcggtgac
     6421 gtggaggaga atcccggccc tatgaccgag tacaagccca cggtgcgcct cgccacccgc
     6481 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     6541 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     6601 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     6661 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     6721 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     6781 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     6841 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     6901 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg
     6961 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7021 acccgcaagc ccggtgcctg aaatcaacct ctggattaca aaatttgtga aagattgact
     7081 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7141 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7201 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7261 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7321 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7381 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7441 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7501 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7561 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7621 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7681 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa aataagatct
     7741 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7801 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7861 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7921 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7981 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     8041 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8101 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     8161 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8221 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8281 ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg
     8341 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8401 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8461 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8521 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     8941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9121 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     9181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9601 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    10381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    10441 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
    10501 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
    10561 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
    10621 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
    10681 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    10741 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg
    10801 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
    10861 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
    10921 tgtaaaacga cggccagtgc caagctg
//
