LOCUS       V018865                 9381 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018865
VERSION     V018865
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..3555
                     /codon_start=1
                     /gene=""
                     /note=""
                     /product="human lysosome-associated membrane glycoprotein
                     1"
                     /protein_id=""
                     /transl_table=1
                     /translation="MAAPGSARRPLLLLLLLLLLGLMHCASA,AMFMVKNGNGTACIMA
                     NFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLN
                     FTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVH
                     MNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDK
                     YNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHS
                     EGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCN
                     AEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLI
                     VLIAYLVGRKRSHAGYQTI*"
                     /label="LAMP1"
     promoter        3602..3813
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             3826..4094
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             4129..4821
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             4822..4875
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             4876..5475
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    5476..6064
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             6138..6371
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    6443..6564
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      6583..6718
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(6756..6772)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6780..6796
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6804..6834)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6849..6870
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7158..7746)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7917..8777)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8778..8882)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     9356..9372
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggcggcc cccggcagcg cccggcgacc cctgctgctg
     2341 ctactgctgt tgctgctgct cggcctcatg cattgtgcgt cagcagcaat gtttatggtg
     2401 aaaaatggca acgggaccgc gtgcataatg gccaacttct ctgctgcctt ctcagtgaac
     2461 tacgacacca agagtggccc taagaacatg acctttgacc tgccatcaga tgccacagtg
     2521 gtgctcaacc gcagctcctg tggaaaagag aacacttctg accccagtct cgtgattgct
     2581 tttggaagag gacatacact cactctcaat ttcacgagaa atgcaacacg ttacagcgtc
     2641 cagctcatga gttttgttta taacttgtca gacacacacc ttttccccaa tgcgagctcc
     2701 aaagaaatca agactgtgga atctataact gacatcaggg cagatataga taaaaaatac
     2761 agatgtgtta gtggcaccca ggtccacatg aacaacgtga ccgtaacgct ccatgatgcc
     2821 accatccagg cgtacctttc caacagcagc ttcagccggg gagagacacg ctgtgaacaa
     2881 gacaggcctt ccccaaccac agcgccccct gcgccaccca gcccctcgcc ctcacccgtg
     2941 cccaagagcc cctctgtgga caagtacaac gtgagcggca ccaacgggac ctgcctgctg
     3001 gccagcatgg ggctgcagct gaacctcacc tatgagagga aggacaacac gacggtgaca
     3061 aggcttctca acatcaaccc caacaagacc tcggccagcg ggagctgcgg cgcccacctg
     3121 gtgactctgg agctgcacag cgagggcacc accgtcctgc tcttccagtt cgggatgaat
     3181 gcaagttcta gccggttttt cctacaagga atccagttga atacaattct tcctgacgcc
     3241 agagaccctg cctttaaagc tgccaacggc tccctgcgag cgctgcaggc cacagtcggc
     3301 aattcctaca agtgcaacgc ggaggagcac gtccgtgtca cgaaggcgtt ttcagtcaat
     3361 atattcaaag tgtgggtcca ggctttcaag gtggaaggtg gccagtttgg ctctgtggag
     3421 gagtgtctgc tggacgagaa cagcatgctg atccccatcg ctgtgggtgg tgccctggcg
     3481 gggctggtcc tcatcgtcct catcgcctac ctcgtcggca ggaagaggag tcacgcaggc
     3541 taccagacta tctagggatc cgcggccgca aggatctgcg atcgctccgg tgcccgtcag
     3601 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     3661 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     3721 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     3781 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct
     3841 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg
     3901 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc
     3961 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc
     4021 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg
     4081 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat gagcgagctg
     4141 atcaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
     4201 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag
     4261 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
     4321 ggcagcaaag ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
     4381 cctgagggct tcacatggga gagaatcacc acatacgaag acgggggcgt gctgaccgct
     4441 acccaggaca ccagcttcca gaacggctgc atcatctaca acgtcaagat caacggggtg
     4501 aacttcccat ccaacggccc tgtgatgcag aagaaaacac gcggctggga ggccaacacc
     4561 gagatgctgt accccgctga cggcggcctg agaggccaca gccagatggc cctgaagctc
     4621 gtgggcgggg gctacctgca ctgctccttc aagaccacat acagatccaa gaaacccgct
     4681 aagaacctca agatgcccgg cttccacttc gtggaccaca gactggaaag aatcaaggag
     4741 gccgacaaag agacctacgt cgagcagcac gagatggctg tggccaagta ctgcgacctc
     4801 cctagcaaac tggggcacag agagggcaga ggaagtcttc taacatgcgg tgacgtggag
     4861 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     4921 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     4981 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     5041 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     5101 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     5161 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     5221 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     5281 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     5341 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     5401 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     5461 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5521 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5581 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5641 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5701 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5761 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5821 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     5881 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5941 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6001 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     6061 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6121 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaaataag atctgctttt
     6181 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6241 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6301 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6361 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6421 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6541 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6601 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6721 gacttttgca gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6781 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6841 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6901 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6961 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7021 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7081 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7141 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7201 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7261 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7321 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7381 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7441 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7501 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7561 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     7621 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7681 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7741 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7801 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7861 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7921 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7981 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8041 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8101 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8161 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8221 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8281 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8341 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8401 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8461 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8521 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8581 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8641 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8701 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8761 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8821 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8881 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     8941 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     9001 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     9061 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     9121 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     9181 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     9241 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     9301 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     9361 acgacggcca gtgccaagct g
//
