LOCUS       V043499                10263 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043499
VERSION     V043499
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..4434
                     /label="Adarb1(NM_001024837)"
                     /note="Adarb1(NM_001024837)"
                     /gene="Adarb1"
     promoter        4484..4695
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4708..4976
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             5011..5703
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             5704..5757
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             5758..6357
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6358..6946
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7020..7253
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7325..7446
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7465..7600
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(7638..7654)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7662..7678
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7686..7716)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7731..7752
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8040..8628)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8799..9659)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9660..9764)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10238..10254
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggatata gaagatgaag agaatatgag ttccagcagc
     2341 actgatatta aagaaaaccg caatctggac aacatgcccc ccaaggacag cagcacacct
     2401 gggcctggcg agggtattcc gctctccaac gggggtggtg gtagcaccag caggaagcgg
     2461 cccctggagg agggcagcaa tggtcactcc aagtaccgcc tgaagaagcg aaggaaaaca
     2521 ccagggcctg ttctgcccaa gaacgccctg atgcagctga acgagatcaa acctggctta
     2581 cagtacatgc tgctgtccca gacaggaccc gtgcatgcac ctctgtttgt catgtctgtg
     2641 gaggtaaacg ggcaggtttt tgagggctct ggccctacaa agaaaaaggc aaaactccat
     2701 gctgctgaga aggccctgag gtcttttgtc cagtttccca acgcctccga ggcccaccta
     2761 gccatgggaa ggaccctctc tgtgaacaca gacttcacgt ctgaccaggc tgacttccct
     2821 gacacactct tcaatggctt cgagactcca gacaagtcag agccaccctt ctacgtaggc
     2881 tccaacgggg atgactcgtt cagctcaagt ggagatgtca gcctatcggc ctccccagtg
     2941 cctgccagcc ttacccagcc tcctctgccc atcccaccac cattcccacc cccaagtggg
     3001 aagaatcccg tgatgatctt gaatgagcta cgcccagggc tgaagtatga cttcctctct
     3061 gagagtgggg agagccacgc caagagcttc gtcatgtccg tggtggtaga tggccagttc
     3121 tttgagggct caggaagaaa caagaagctt gccaaggccc gggctgcaca gtctgccttg
     3181 gctactgtct tcaatttgca cttggaccaa acaccatctc gccagcctgt cctcagtgag
     3241 ggtcttcagt tgcatttgcc acaggtattg gcagatgctg tctcccgcct ggtcctgggt
     3301 aagttcagtg acctgacaga caacttttcc tcccctcatg cacgaaggaa agtgctctct
     3361 ggagtagtga tgaccacagg tacagatgtc aaagatgcca aggtgataag tgtttcgaca
     3421 gggacgaagt gtatcaacgg tgaatacatg agtgaccgtg gcctcgcact caatgactgc
     3481 cacgcagaga taatctcccg aaggtccctg ctcaggtttc tttatgcaca gctcgagctt
     3541 tatttaaata acaaagaaga ccagaaaaag tccatatttc agaagtcaga gcggggtggg
     3601 ttccggctga aggataccgt gcagttccac ctgtacatca gcacctcgcc ctgcggagac
     3661 gccagaatat tctctcccca cgagcccgtg ctagagggta tgacgccaga ctctcaccag
     3721 ctgacagaac cagcagatag acatccgaat cgcaaagcaa ggggacagct acggacgaaa
     3781 atagagtctg gcgaggggac aatccctgtg cgctcaaatg ccagcatcca gacctgggac
     3841 ggggtgctgc agggggaacg gctgctcacc atgtcctgca gtgacaagat agcacgctgg
     3901 aacgtggtgg gcatccaggg gtccctgctc agcattttcg tggaacccat ctacttctcc
     3961 agcatcatct tgggcagcct gtaccacggg gaccacctct ccagggccat gtaccagcgg
     4021 atctccaaca tagaggacct gccaccgctc tacactctca acaagcccct gctcagcggc
     4081 atcagcaatg cagaggcacg gcagccaggg aaggcaccca acttcagtgt caactggaca
     4141 gtgggtgacg ccaccattga ggtcatcaat gccacaacag ggaaggatga gcttggccgc
     4201 ccatcccgcc tgtgtaagca cgcgctgtac tgtcgctgga tgcgtgtaca cggcaaggtt
     4261 cccccccacc tgctgcgcac caagatcact aagcctacca cataccacga gtccaagctg
     4321 gcagcgaggg agtaccaggc tgccaaggcc cgtctgttca ctgccttcat caaggcgggg
     4381 ctgggcgcct gggtggagaa gcccacagag caggaccagt tctccttcac tccctgagga
     4441 tccgcggccg caaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     4501 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     4561 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     4621 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     4681 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     4741 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     4801 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     4861 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     4921 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     4981 ctgtgaccgg cgcctacgct agacgccacc atgagcgagc tgatcaagga gaacatgcac
     5041 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc
     5101 gaaggcaagc cctacgaggg cacccagacc atgaagatca aggtggtcga gggcggccct
     5161 ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa agccttcatc
     5221 aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg
     5281 gagagaatca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcttc
     5341 cagaacggct gcatcatcta caacgtcaag atcaacgggg tgaacttccc atccaacggc
     5401 cctgtgatgc agaagaaaac acgcggctgg gaggccaaca ccgagatgct gtaccccgct
     5461 gacggcggcc tgagaggcca cagccagatg gccctgaagc tcgtgggcgg gggctacctg
     5521 cactgctcct tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
     5581 ggcttccact tcgtggacca cagactggaa agaatcaagg aggccgacaa agagacctac
     5641 gtcgagcagc acgagatggc tgtggccaag tactgcgacc tccctagcaa actggggcac
     5701 agagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     5761 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     5821 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     5881 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     5941 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6001 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6061 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6121 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6181 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     6241 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     6301 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaaat
     6361 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     6421 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     6481 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     6541 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     6601 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     6661 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     6721 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     6781 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     6841 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     6901 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg tacctttaag
     6961 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7021 ggaagggcta attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct
     7081 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7141 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7201 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7261 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7321 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7381 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7441 atcttatcat gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct
     7501 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7561 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc
     7621 ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     7681 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7741 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7801 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     7861 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     7921 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     7981 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8041 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8101 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8161 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8221 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8281 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8341 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8401 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8461 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8521 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     8581 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     8641 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     8701 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     8761 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     8821 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     8881 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     8941 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9001 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9061 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9121 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9181 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9241 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9301 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9361 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9421 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9481 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9541 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     9601 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     9661 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     9721 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9781 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     9841 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     9901 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     9961 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
    10021 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    10081 aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
    10141 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
    10201 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
    10261 ctg
//
