LOCUS       V043239                11664 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V043239
VERSION     V043239
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2010..2213
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2302..5835
                     /label="Adar(NM_001146296)"
                     /note="Adar(NM_001146296)"
                     /gene="Adar"
     promoter        5885..6096
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             6109..6377
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     CDS             6412..7104
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="red fluorescent protein from Entacmaea
                     quadricolor"
                     /transl_table=1
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
                     /label="TurboRFP"
     CDS             7105..7158
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             7159..7758
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    7759..8347
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             8421..8654
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    8726..8847
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      8866..9001
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(9039..9055)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9063..9079
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9087..9117)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    9132..9153
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(9441..10029)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(10200..11060)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11061..11165)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     11639..11655
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catgtctcaa gggttcaggg gacccacagg ggtgttccct
     2341 caccagacac agtcgtactt ggaccctagt catgagcata gcaagtggag atacccgcag
     2401 ccacaggggc cggagtctta ccctaggagt ttccagcttc agcagataga gtttctcaaa
     2461 gggcggctcc cagaagcacc cttgattgga atacaaaccc agtcactgcc gccattcctc
     2521 ccaggacact ggccaagatt cccagggcca cctgcccaag acaggcaact ggaaatctgg
     2581 gagttcccca ggagtgtgac tctcagaaat caggggttcc acataggacc cccacttcct
     2641 cccccacaca gcaggggtac accatggaga ggtgctgacg ggctttgctc acacttccgg
     2701 gagctgagca tcagtcagag tccggagcag aaggttctaa accgcctgga agagcttggg
     2761 gaggggaagg ccaccactgc ccatgtgcta gccagagagc tcagaatccc caaaagggac
     2821 atcaatcgta ttttgtactc cctggaaaag aagggaaagc tgcacagagg aagggggaaa
     2881 cctcctttgt ggagccttgt gcccttgagt caggcttgga ctcagccccc tggagttgtg
     2941 aatccagata gttgtatcca ggaattccct agaggagagc ctggtttgga cagtgaggac
     3001 ggagaccctg cctctgactt agaaggacct tctgagcctc ttgacatggc tgaaatcaag
     3061 gagaagatct gtgactatct gttcaatgtg tcaaactcct ctgccctgaa cctggctaag
     3121 aacattggcc tcaccaaggc ccgagatgtg acctcagtgc tgattgactt ggaaaggcaa
     3181 ggcgatgtct acaggcaagg ggcaactcct cccatctggt acttgacgga caagaagcgt
     3241 gagaggctgc agatgaagag aagtacacac agtgctcctg cccctacccc gacagctgtc
     3301 ccagaggcca ctagaagccc ctcattccct gcctgccacc cgcccccagc aggtgcctca
     3361 agcagtgtgg cagcctccaa gagagtggag aatgggcagg agcctgcgat aaagcatgaa
     3421 agtaggcatg aggccagacc aggaccaatg agactgcggc ctcacgctta tcacaatggc
     3481 ccctctagag cagggtatgt ggcctctgaa aatggccagt gggccacaga tgacatccca
     3541 gataacttga atagtatcca cacagcacca ggtgagtttc gagccatcat ggagatgccc
     3601 tccttctaca gccctacctt gccacggtgt tcaccctaca agaagctaac tgagtgccag
     3661 ctgaagaacc ctgtcagcgg gttgttagag tatgctcagt tcactagtca gacctgtgat
     3721 ttcaacctga tagagcagag tggaccgtcc catgaacctc gatttaaatt ccaggttgtc
     3781 atcaatgggc gggaatttcc cccagctgag gctggcagca agaaagtagc caagcaggac
     3841 gcagcagtga aagccatggc gattctgctt cgggaagcca aagccaaaga cagtggtcaa
     3901 ccagaagact tgtcccactg tcccatggaa gaagactcgg agaaaccagc agaggctcag
     3961 gcccccagct cctcagcaac atccttgttc tctgggaaga gcccagttac tacactgctt
     4021 gagtgcatgc acaaactagg gaactcctgt gaattccgtc tcctgtccaa agaaggccct
     4081 gctcatgacc ccaagttcca gtactgtgta gcagtaggag cccagacctt cccccctgtg
     4141 agcgccccca gcaagaaggt agcaaagcag atggccgcag aggaagccat gaaggcgctg
     4201 caggaggagg cagccagttc agcggatgac cagtctggag gtgcgaacac agactcactt
     4261 gatgaatcta tggctcccaa caagatcagg aggattggtg agctcgtcag gtacctgaac
     4321 accaaccccg taggcggctt gttggagtac gcccgatctc atggctttgc tgctgagttc
     4381 aagctcattg accagtctgg acctcctcac gaacccaagt ttgtttacca agcaaaagtt
     4441 gggggccgct ggtttccagc cgtgtgtgca cacagcaaga aacagggcaa gcaggatgca
     4501 gcggatgcag ccctccgggt cttgatcggg gagagcgaga aggcagagca gttgggtttc
     4561 gcagaggtaa ccccagtaac aggggccagt ctcagaagaa ctatgctcct cctttccagg
     4621 tccccagatg cacatccaaa gacacttcct ctctctggca gcaccttcca cgaccagata
     4681 gctatgctga gccacaggtg cttcaatgct ctgaccaaca gtttccagcc ctccctgctc
     4741 ggccgcaaga tcctggctgc cattattatg aaaagagatc cagaggacat gggtgttgtc
     4801 gtgagtttgg ggacaggcaa tcgctgtgtg aaaggggact ctctcagcct gaagggagag
     4861 acggtcaatg actgccatgc cgaaatcatc tcccggaggg gcttcatcag gtttctctac
     4921 agtgaactga tgaagtacaa ccaccacact gccaagaaca gcatatttga gcttgccagg
     4981 ggaggagaga agctgcagat aaagaagacg gtttcttttc atctctacat cagcacggcg
     5041 ccatgtggag atggggccct ctttgacaaa tcctgcagtg accgtgccgt ggaaagcaca
     5101 gagtcccgcc attaccctgt ctttgaaaat cccaagcaag gcaagcttcg caccaaggtg
     5161 gagaatgggg aaggcacaat tcctgtggag tccagtgata ttgtacccac gtgggatggc
     5221 atccgccttg gggaaagact ccgtaccatg tcctgtagtg acaaaatcct acgctggaat
     5281 gtgctgggcc tgcaaggggc gttgttgacg cacttcctac agcctgtgta cctgaaatct
     5341 gtaaccttag gttacctttt cagccaaggg catctgaccc gtgctatttg ctgccgcgtg
     5401 accagagatg ggaaagcatt tgaggatgga ctacgctatc cctttattgt caaccacccc
     5461 aaggtcggcc gagtcagtgt ttatgattcc aaaagacagt ccggaaagac caaggaaaca
     5521 agcgtcaact ggtgcatggc tgatggctat gacctagaga tcctggatgg caccagaggc
     5581 actgtggatg gaccagggaa agagttgtct cgggtgtcca agaagaatat tttccttcag
     5641 tttaagaagc tctgctcctt ccgagcccgc agagatttac tgcagctctc ttatggtgaa
     5701 gccaagaaag ctgcccgtga ctatgactta gccaagaact acttcaagaa aagcctgcga
     5761 gacatgggct atgggaattg gatcagcaaa ccccaggagg aaaagaactt ttacctctgt
     5821 ccagtaccca atgactgagg atccgcggcc gcaaggatct gcgatcgctc cggtgcccgt
     5881 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5941 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6001 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6061 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     6121 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     6181 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     6241 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     6301 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     6361 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac catgagcgag
     6421 ctgatcaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     6481 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgaagatc
     6541 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     6601 tacggcagca aagccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     6661 ttccctgagg gcttcacatg ggagagaatc accacatacg aagacggggg cgtgctgacc
     6721 gctacccagg acaccagctt ccagaacggc tgcatcatct acaacgtcaa gatcaacggg
     6781 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cacgcggctg ggaggccaac
     6841 accgagatgc tgtaccccgc tgacggcggc ctgagaggcc acagccagat ggccctgaag
     6901 ctcgtgggcg ggggctacct gcactgctcc ttcaagacca catacagatc caagaaaccc
     6961 gctaagaacc tcaagatgcc cggcttccac ttcgtggacc acagactgga aagaatcaag
     7021 gaggccgaca aagagaccta cgtcgagcag cacgagatgg ctgtggccaa gtactgcgac
     7081 ctccctagca aactggggca cagagagggc agaggaagtc ttctaacatg cggtgacgtg
     7141 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     7201 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     7261 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     7321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     7381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     7441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     7501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     7561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7621 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     7741 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     7801 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     7861 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     7921 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     7981 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8041 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8101 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     8161 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8221 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8281 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8341 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     8401 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct
     8461 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     8521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     8581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     8641 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa
     8701 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata
     8761 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8821 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact
     8881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     9001 ctagactttt gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga
     9061 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9121 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9181 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9241 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9841 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11161 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    11221 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
    11281 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    11341 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    11401 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    11461 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
    11521 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    11581 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    11641 aaaacgacgg ccagtgccaa gctg
//
