LOCUS       V013207                 9458 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013207
VERSION     V013207
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             234..414
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1920
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2010..2213
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        4001..4212
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     LTR             4225..4493
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4521..5543
                     /label="HygR"
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    5553..6141
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6215..6448
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6520..6654
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6660..6795
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6833..6849)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6857..6873)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6881..6911)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6926..6947)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7235..7823)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7997..8854)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8855..8959)
                     /label="AmpR promoter"
     primer_bind     9433..9449
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
     2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
     2281 agagctagcg aattcgccac catggaggac gccaagaaca tcaagaaggg ccccgccccc
     2341 ttctaccccc tggaggacgg caccgccggc gagcagctgc acaaggccat gaagcgctac
     2401 gccctggtgc ccggcgccat cgccttcacc gacgcccaca tccaggtgga cgtgacctac
     2461 gccgagtact tcgagatgag cgtgcgcctg gccgaggcca tgcgccgcta cggcctgaac
     2521 accaaccacc gcatcgtggt gtgcagcgag aacagcagcc agttcttcat gcccgtgctg
     2581 ggcgccctgt tcatcggcgt ggccgtggcc cccgccaacg acatctacaa cgagcgcgag
     2641 ctgctgaaca gcatgggcat cagccagccc accgtggtgt tcgtgagcaa gaagggcctg
     2701 cgcaaggtgc tgaacgtgca gaagaagctg cccatcatcc gcaagatcat catcatggac
     2761 agcaagaccg actaccaggg cttccagagc atgtacacct tcgtgaccag ccacctgccc
     2821 cccagcttca acgagtacga cttcgtgccc gagagcttcg accgcgacaa gaccatcgcc
     2881 ctgatcatga acagcagcgg cagcaccggc ctgcccaagg gcgtggccct gccccaccgc
     2941 accgcctgcg tgcgcttcag ccacgcccgc gaccccatct tcggctacca gaacatcccc
     3001 gacaccgcca tcctgagcgt ggtgcccttc caccacggct tcggcatgtt caccaccctg
     3061 ggctacctga tctgcggctt ccgcgtggtg ctgatgtacc gcttcgagga ggagctgttc
     3121 ctgcgcagcc tgcaggacta caagatccag agcgccctgc tggtgcccac cctgttcagc
     3181 tgcctggcca agagcaccct gatcgacaag tacgacctga gcagcctgcg cgagatcgcc
     3241 agcggcggcg cccccctgag caaggaggtg ggcgaggccg tggccaagcg cttccgcctg
     3301 cccggcatcc gccagggcta cggcctgacc gagaccacca acgccgtgat gatcaccccc
     3361 gagggcgacc gcaagcccgg cagcgtgggc aaggtggtgc ccttcttcga ggccaaggtg
     3421 gtggacctgg tgaccggcaa gaccctgggc gtgaaccagc gcggcgagct gtgcgtgcgc
     3481 ggccccatga tcatgagcgg ctacgtgaac aaccccgagg ccaccaacgc cctgatcgac
     3541 aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga gcacttcttc
     3601 atcgtggacc gcctgaagag cctgatcaag tacaagggct accaggtggc ccccgccgag
     3661 ctggagggca tcctgctgca gcacccctac atcttcgacg ccggcgtggc cggcctgccc
     3721 gacgacgacg ccggcgagct gcccgccgcc gtggtggtgc tggagcacgg caagaccatg
     3781 accgagaagg agatcgtgga ctacgtggcc agccaggtga ccaccgccaa gaagctgcgc
     3841 ggcggcgtgg tgttcgtgga cgaggtgccc cgcggcagca ccggcaagct ggacgcccgc
     3901 aagatccgcg agatcctgac caaggccaag aaggacggca agatcgccgt gtgaggatcc
     3961 gcggccgcaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     4021 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     4081 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     4141 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     4201 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     4261 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     4321 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     4381 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     4441 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     4501 tgaccggcgc ctacgtcgac atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     4561 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     4621 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     4681 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     4741 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     4801 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     4861 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     4921 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     4981 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5041 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5101 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     5161 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     5221 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     5281 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     5341 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     5401 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     5461 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     5521 gcactcgtcc gagggcaaag gaataggtcg acaatcaacc tctggattac aaaatttgtg
     5581 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5641 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5701 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5761 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5821 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5881 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5941 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6001 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6061 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6121 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta
     6181 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     6241 aaataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     6301 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6361 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6421 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     6481 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     6541 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     6601 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     6661 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6721 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6781 gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc
     6841 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6901 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6961 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7021 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     7681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9001 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     9061 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9121 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9181 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9241 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     9301 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9361 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9421 agtcacgacg ttgtaaaacg acggccagtg ccaagctg
//
