LOCUS       V014051                 9040 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014051
VERSION     V014051
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..233
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    458..583
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1076..1309
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1798..1915
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     intron          2578..3595
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             3661..3750
                     /label="3xHA"
                     /note="three tandem HA epitope tags"
     misc_feature    3788..4365
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4398..5114
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    5135..5723
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5797..6030
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6102..6236
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6242..6377
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     primer_bind     complement(6415..6431)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6439..6455)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6463..6493)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6508..6529)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6817..7405)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7579..8436)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8437..8541)
                     /label="AmpR promoter"
     primer_bind     9015..9031
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     1981 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2041 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2101 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     2161 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     2221 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     2281 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     2341 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     2401 ggggcggggg gggggggggg gcccccccca ggcggggcgg ggcggggcga ggggcggggc
     2461 ggggcgaggc ggaaaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     2521 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     2581 gtcgttgcgc gctgccttcc ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     2641 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     2701 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     2761 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
     2821 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
     2881 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     2941 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     3001 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     3061 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     3121 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     3181 ccgcctcggg ccggggaggg ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg
     3241 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     3301 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     3361 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     3421 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     3481 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     3541 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     3601 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgc caccatggct
     3661 tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga
     3721 tcctatccat atgacgttcc agattacgct ctcgagaatt cgaatttaaa tcggatccgc
     3781 ggccgcgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     3841 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     3901 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     3961 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     4021 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     4081 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     4141 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctcacc tcaagcgtat
     4201 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     4261 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
     4321 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggtgagc aagggcgagg
     4381 aggataacat ggccatcatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     4441 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4501 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4561 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     4621 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4681 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4741 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4801 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     4861 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     4921 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4981 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     5041 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     5101 acgagctgta caagtaatgt acaagtgagt cgacaatcaa cctctggatt acaaaatttg
     5161 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     5221 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     5281 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     5341 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5401 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5461 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5521 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     5581 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5641 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5701 ctccctttgg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg
     5761 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     5821 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     5881 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     5941 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6001 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6061 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6241 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6301 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6361 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata
     6421 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6481 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6541 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6601 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6661 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     7261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7441 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7501 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7561 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7621 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7681 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7741 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7801 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7861 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7921 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7981 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8041 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8101 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8161 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     8221 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     8281 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8341 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8401 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8461 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8521 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     8581 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc
     8641 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     8701 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     8761 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     8821 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     8881 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     8941 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     9001 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//
