LOCUS       40924_9426        3682 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCDF-SP6p(-11C)-SpacerB-lacI-LVA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Schaerli Y, Munteanu A, Gili M, Cotterell J, Sharpe J, Isalan M.
  TITLE     A unified design space of synthetic stripe-forming networks
  JOURNAL   Nat Commun 5, 4905 (2014)
  PUBMED    25247316
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Schaerli Y, Munteanu A, Gili M, Cotterell J, Sharpe J, Isalan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2014) Centre for Genomic Regulation (CRG), 
            EMBL/CRG Systems Biology Research Unit, Dr. Aiguader 88, Barcelona, 
            Barcelona 08003, Spain
REFERENCE   3  (bases 1 to 3682)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3682)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            5, 4905 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JUL-2014) Centre for Genomic Regulation (CRG), EMBL/CRG Systems 
            Biology Research Unit, Dr. Aiguader 88, Barcelona, Barcelona 08003, 
            Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(117..905)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(906..997)
                     /label=AmpR promoter
     rep_origin      complement(1045..1783)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     1946..1962
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1975..1993
                     /gene="lacI-LVA"
                     /label=SP6(-11C) promoter
                     /note="SP6(-11C) promoter"
                     /regulatory_class="promoter"
     misc_feature    1994..2027
                     /gene="lacI-LVA"
                     /label=SpacerB
                     /note="SpacerB"
     RBS             2084..2106
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2114..3193
                     /label=lacI
                     /note="lac repressor"
     CDS             3200..3232
                     /label=ssrA tag (LVA)
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
     terminator      3406..3453
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3462..3548
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3640..3667
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc
       61 gggtcatcgt ggccggatct tgcggcccct cggcttgaac gaattgttag acattatttg
      121 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
      181 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
      241 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
      301 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
      361 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
      421 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
      481 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
      541 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
      601 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
      661 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
      721 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
      781 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
      841 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
      901 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
      961 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag ctagctcact cggtcgctac
     1021 gctccgggcg tgagactgcg gcgggcgctg cggacacata caaagttacc cacagattcc
     1081 gtggataagc aggggactaa catgtgaggc aaaacagcag ggccgcgccg gtggcgtttt
     1141 tccataggct ccgccctcct gccagagttc acataaacag acgcttttcc ggtgcatctg
     1201 tgggagccgt gaggctcaac catgaatctg acagtacggg cgaaacccga caggacttaa
     1261 agatccccac cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg
     1321 tttaccggat acctgttccg cctttctccc ttacgggaag tgtggcgctt tctcatagct
     1381 cacacactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga
     1441 actccccgtt cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc
     1501 ggaaaagcac ggtaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga
     1561 tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga
     1621 aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca
     1681 actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa
     1741 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgct
     1801 ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata
     1861 taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     1921 ctcctaggat gcagtgactc gagttgtaaa acgacggcca gtcaattgga attcatttag
     1981 ctgacactat agaaggggcc aagcaggggg ccaagcaggg ggccaaggag ctcgttgagt
     2041 tacctgcagc gattgtacta gttgcggccg cggtaccaat aattttgttt aactttaaga
     2101 aggagatata catatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
     2161 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     2221 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
     2281 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
     2341 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
     2401 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
     2461 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
     2521 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
     2581 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
     2641 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
     2701 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
     2761 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
     2821 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
     2881 cattaccgag tccgggctgc gcgttggtgc ggacatctcg gtagtgggat acgacgatac
     2941 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
     3001 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     3061 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     3121 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     3181 ggaaagcggg cagaggcctg ctgctaacga tgaaaactat gctctggtcg cctaataaag
     3241 gccttaataa ggatcccctg caggacctca gcaatcgctt ggaccagctt tcttaagggt
     3301 acctattaca gctggatccg gcgccgctca ttcgctaatc gccacgacgc gtagtcgacc
     3361 cgccaattcc catggggcgc gcctgctgcc accgctgagc aataactagc ataacccctt
     3421 ggggcctcta aacgggtctt gaggggtttt ttgccaggca tcaaataaaa cgaaaggctc
     3481 agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     3541 ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga gggtggcggg
     3601 caggacgccc gccataaact gccaggcatc aaactaagca gaaggccatc ctgacggatg
     3661 gcctttttgc gtttcagatc ta
//
