LOCUS       40924_9406        3677 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCDF-J23100-TetO-lacI-LVA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Schaerli Y, Munteanu A, Gili M, Cotterell J, Sharpe J, Isalan M.
  TITLE     A unified design space of synthetic stripe-forming networks
  JOURNAL   Nat Commun 5, 4905 (2014)
  PUBMED    25247316
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Schaerli Y, Munteanu A, Gili M, Cotterell J, Sharpe J, Isalan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2014) Centre for Genomic Regulation (CRG), 
            EMBL/CRG Systems Biology Research Unit, Dr. Aiguader 88, Barcelona, 
            Barcelona 08003, Spain
REFERENCE   3  (bases 1 to 3677)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3677)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            5, 4905 (2014)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-JUL-2014) Centre for Genomic Regulation (CRG), EMBL/CRG Systems 
            Biology Research Unit, Dr. Aiguader 88, Barcelona, Barcelona 08003, 
            Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(117..905)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(906..997)
                     /label=AmpR promoter
     rep_origin      complement(1045..1783)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     1946..1962
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1975..2009
                     /gene="lacI-LVA"
                     /label=J23100 promoter
                     /note="J23100 promoter"
                     /regulatory_class="promoter"
     protein_bind    2016..2034
                     /label=tet operator
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2142..2164
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2172..3251
                     /label=lacI
                     /note="lac repressor"
     CDS             3258..3290
                     /label=ssrA tag (LVA)
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
     terminator      3401..3448
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3457..3543
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3635..3662
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc
       61 gggtcatcgt ggccggatct tgcggcccct cggcttgaac gaattgttag acattatttg
      121 ccgactacct tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg
      181 cgcgaggcca agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg
      241 ggctgatact gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg
      301 attttgccgg ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca
      361 tcgccagccc agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat
      421 agatcctgtt caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg
      481 ctatgttctc ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg
      541 aagatacctg caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct
      601 ggataacgcc acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga
      661 atctcgctct ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc
      721 gttgtttcat caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc
      781 aggccgccat ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg
      841 cgctcgatga cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc
      901 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
      961 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag ctagctcact cggtcgctac
     1021 gctccgggcg tgagactgcg gcgggcgctg cggacacata caaagttacc cacagattcc
     1081 gtggataagc aggggactaa catgtgaggc aaaacagcag ggccgcgccg gtggcgtttt
     1141 tccataggct ccgccctcct gccagagttc acataaacag acgcttttcc ggtgcatctg
     1201 tgggagccgt gaggctcaac catgaatctg acagtacggg cgaaacccga caggacttaa
     1261 agatccccac cgtttccggc gggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg
     1321 tttaccggat acctgttccg cctttctccc ttacgggaag tgtggcgctt tctcatagct
     1381 cacacactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga
     1441 actccccgtt cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc
     1501 ggaaaagcac ggtaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga
     1561 tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga
     1621 aggacagatt tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca
     1681 actgacttaa ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa
     1741 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgct
     1801 ctagatttca gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata
     1861 taagttgtaa ttctcatgtt agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     1921 ctcctaggat gcagtgactc gagttgtaaa acgacggcca gtcaattgga attcttgacg
     1981 gctagctcag tcctaggtac agtgctagcg aagggtccct atcagtgata gagagagctc
     2041 gttgagttac ctgcagcgat tgtactagtt gcggccgcct gcgcaccacc tgcaggacct
     2101 cagcaatcgc ttggaccagc tttcttaagg gtaccaataa ttttgtttaa ctttaagaag
     2161 gagatataca tatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     2221 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     2281 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     2341 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     2401 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     2461 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     2521 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     2581 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     2641 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     2701 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     2761 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     2821 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     2881 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     2941 ttaccgagtc cgggctgcgc gttggtgcgg acatctcggt agtgggatac gacgataccg
     3001 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     3061 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     3121 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     3181 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3241 aaagcgggca gaggcctgct gctaacgatg aaaactatgc tctggtcgcc taataaaggc
     3301 cttaataagg atccggcgcc gctcattcgc taatcgccac gacgcgtagt cgacccgcca
     3361 attcccatgg ggcgcgcctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     3421 ctctaaacgg gtcttgaggg gttttttgcc aggcatcaaa taaaacgaaa ggctcagtcg
     3481 gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     3541 aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggagggtg gcgggcagga
     3601 cgcccgccat aaactgccag gcatcaaact aagcagaagg ccatcctgac ggatggcctt
     3661 tttgcgtttc agatcta
//
