LOCUS       V008640                 4991 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008640
VERSION     V008640
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Kim B, Binkley R, Kim HU, Lee SY.
  TITLE     Metabolic engineering of Escherichia coli for the enhanced
            production of l-tyrosine
  JOURNAL   Biotechnol. Bioeng. (2018) In press
   PUBMED   30019750
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
            Korea Advanced Institute of Science and Technology, Daehak-ro 291,
            Daejeon 34141, South Korea
REFERENCE   3  (bases 1 to 4991)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4991)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng. (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
            Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
            34141, South Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             71..1279
                     /gene="PKS3"
                     /label="Aloesone synthase"
                     /note="Aloesone synthase from Aloe arborescens. Accession#:
                     C4MBZ5"
     CDS             1293..1310
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        1424..1442
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1443..1467
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1576..1620
                     /label="S-Tag"
                     /note="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
     terminator      1672..1719
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(1893..2681)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(2682..2773)
                     /label="AmpR promoter"
     rep_origin      complement(2821..3559)
                     /direction=LEFT
                     /label="CloDF13 ori"
                     /note="Plasmids containing the CloDF13 (CDF) origin of
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     protein_bind    complement(3735..3756)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3772..4851)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4852..4929)
                     /label="lacI promoter"
     regulatory      4975..4991
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
       61 gagatatacc atgggttcac tctccgactc tacgcctttg atgaaggatg tgcagggcat
      121 ccgaaaggcc caaaaggccg acggaactgc gaccgtgatg gccattggaa cagctcaccc
      181 tcctcatatc atttcgcagg acagctacgc ggatttctac ttccgggtca ccaacagcga
      241 gcacaaggtc gagctcaaga agaagttcga tcgcatctgc aagaaaacga tgataggcaa
      301 gcgttacttt aacttcgacg aggagttctt gaagaagtat cccaacatta cttcatttga
      361 caagcccagc ctcaacgaca ggcatgacat ttgcattcct ggggtgccgg cgctgggggc
      421 ggaagcggct gttaaagcca ttgaggagtg gggacgccct aagtctgaga tcactcacct
      481 cgtcttctgc accagcggcg gtgttgacat gcctagtgcc gatttccagt gcgctaagct
      541 ccttggcctc cgcaccaatg tcaacaaata ctgcatctac atgcagggat gctatgctgg
      601 tggcaccgtc atgcggtatg ccaaggatct ggccgagaac aaccgtggtg ctcgtgttct
      661 gatggtgtgc gcagagctca ccatcattgc gctccgtggc cccaatgatt cccatatcga
      721 caatgctatc ggcaactctc ttttcggaga tggagctgct gcgctgattg ttgggtcaga
      781 ccccatcatc ggtgtcgaga agcccatgtt cgagattgtc tgcgcaaagc agactgtgat
      841 cccaaacagt gaagaagtta tccatctcca cctgagggag tcgggtctga tgttctacat
      901 gaccaaggac agcgccgcga ccatctccaa taatatagag gcctgccttg ttgatgtgtt
      961 taagtcggtg gggatgactc ctccggagga ttggaactct ctcttctgga tccctcatcc
     1021 cggtggccgg gctatcctcg accaagttga ggccaagctg aagcttcgcc ccgagaagtt
     1081 tagtgcgact cgaacagttc tctgggatta tggcaacatg ataagtgcat gtgtgctcta
     1141 tatactggat gagatgagaa gaaaatctgc tgctgaagga ttagaaacct acggagaggg
     1201 gttagagtgg ggtgtcttgc ttggttttgg accaggaatg actattgaaa ctatccttct
     1261 tcatagcctg cctcctgtgt aatggcagca gccatcacca tcatcaccac agccaggatc
     1321 cgaattcgag ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc
     1381 gaacagaaag taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata
     1441 ggggaattgt gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga
     1501 gatatacata tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt
     1561 accctcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg
     1621 tctactagcg cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa
     1681 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag
     1741 aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa
     1801 gcggctattt aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg
     1861 gcccctcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct
     1921 ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct
     1981 tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc
     2041 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
     2101 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
     2161 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
     2221 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
     2281 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
     2341 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
     2401 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
     2461 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
     2521 accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg
     2581 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
     2641 gatagttgag tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat
     2701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     2761 aaaaataaac aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg
     2821 gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg
     2881 tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca
     2941 gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg
     3001 aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt
     3061 cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt
     3121 tctcccttac gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt
     3181 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg
     3241 cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact
     3301 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
     3361 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
     3421 tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc
     3481 acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
     3541 tcaagaagat cctttgatct tttctactga accgctctag atttcagtgc aatttatctc
     3601 ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc
     3661 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
     3721 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
     3781 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3841 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     3901 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     3961 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     4021 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
     4081 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     4141 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     4201 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     4261 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     4321 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     4381 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     4441 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     4501 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     4561 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     4621 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     4681 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     4741 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     4801 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     4861 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     4921 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aaattaatac
     4981 gactcactat a
//
