LOCUS       40924_9366       10295 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector pCD341, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10295)
  AUTHORS   Dehio M, Knorre A, Lanz C, Dehio C.
  TITLE     Construction of versatile high-level expression vectors for 
            Bartonella henselae and the use of green fluorescent protein as a 
            new expression marker
  JOURNAL   Gene 215 (2), 223-229 (1998)
  PUBMED    9714815
REFERENCE   2  (bases 1 to 10295)
  AUTHORS   Dehio C, Dehio M, Knorre A, Lanz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-1998) Infektionsbiologie, Max-Planck-Institut fuer
            Biologie, Spemannstrasse 34, Tuebingen D-72076, Germany
REFERENCE   3  (bases 1 to 10295)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10295)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1998"; volume: "215"; issue: "2"; pages: "223-229"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAR-1998) Infektionsbiologie, Max-Planck-Institut fuer Biologie,
            Spemannstrasse 34, Tuebingen D-72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     Name: SYNPCD341.
FEATURES             Location/Qualifiers
     source          1..10295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(37..53)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      392..478
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      570..597
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        616..707
                     /label=AmpR promoter
     promoter        1158..1260
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1750..2541
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     rep_origin      complement(3236..3630)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(3657..3941)
                     /codon_start=1
                     /gene="mobC"
                     /product="conjugational DNA transfer protein"
                     /label=mobC
                     /protein_id="AAC35767.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRERAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            complement(3657..3941)
                     /gene="mobC"
                     /label=mobC
     oriT            3972..4059
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             4888..5301
                     /codon_start=1
                     /gene="mobB"
                     /product="conjugal DNA transfer protein"
                     /label=mobB
                     /protein_id="AAC35769.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTKQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            4888..5301
                     /gene="mobB"
                     /label=mobB
     CDS             5298..6266
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             6330..6542
                     /codon_start=1
                     /product="unknown"
                     /label=unknown
                     /note="E"
                     /protein_id="AAC35771.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             6544..6750
                     /codon_start=1
                     /gene="cac"
                     /product="Cac"
                     /function="regulates repC and repA gene expression"
                     /label=cac
                     /protein_id="AAC35772.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     gene            6544..6750
                     /gene="cac"
                     /label=cac
     CDS             6780..7616
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             7606..8454
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(8877..9692)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /protein_id="AAC35776.1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTTWTACCNSLRARR"
     gene            complement(8877..9692)
                     /gene="lacI"
                     /label=lacI
     promoter        complement(9816..9893)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        10123..10151
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    10159..10175
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        10205..10235
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10243..10259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10267..10283
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
       61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
      241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
      301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
      361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
      541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtgatg
      901 tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
      961 gatagaaaca gaagccactg gagcaagcac ctcaaaaaca ccatcataca ctaaatcagt
     1021 aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
     1081 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
     1141 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
     1201 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
     1261 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     1321 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     1381 attacggcct ttttagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg
     1441 aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc
     1501 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt
     1561 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg
     1621 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc
     1681 ttgccgccaa ggatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga
     1741 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1801 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1861 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1921 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1981 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2041 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     2101 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     2161 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     2221 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     2281 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     2341 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     2401 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     2461 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     2521 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     2581 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     2641 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     2701 agttcttcgc ccaccccaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     2761 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     2821 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     2881 gatgagtggc agggcggggc gtaatttttt taaggcagtt attgtcttca aattcccgtt
     2941 gcacatagcc cggcaattcc tttccctgct ctgccataag cgcagcgaat gccgggtaat
     3001 actcgtcaac gatctgatag agaagggttt gctcgggtcg gtggctctgg taacgaccag
     3061 tatcccgatc ccggctggcc gtcctggccg ccacatgagg catgttccgc gtccttgcaa
     3121 tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc agccgaaatg
     3181 cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac tgtcacgaac
     3241 ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc aataactgtc
     3301 acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg gaaaaatcca
     3361 tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg tggggcgctg
     3421 ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg ctgcgctagg
     3481 ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc gctaaacccc
     3541 acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg cctttccccc
     3601 tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc gatcattcag
     3661 cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg gtcgcgttca
     3721 aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct gttcaccttg
     3781 gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg ctcttgctgc
     3841 tgttcccttg cccgctcccg ctgaatttcg gcattgattc gcgctcgttg ttcttcgagc
     3901 ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc ccattgttaa
     3961 tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc ctactttgta
     4021 ggggagggcg cacttaccgg tttctcttcg agaaactggc ctaacggcca cccttcgggc
     4081 ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca aaagcaacag cgaggcagca
     4141 tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc gggcggccaa tcggccaggg
     4201 ccaaggccga ctacatccag cgcgaaggca agtatgcccg cgacatggat gaagtcttgc
     4261 acgccgaatc cgggcacatg ccggagttcg tcgagcggcc cgccgactac tgggatgctg
     4321 ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga ggtcgaattt gccctgccgg
     4381 tcgagctgac cctcgaccag cagaaggcgc tggcgtccga gttcgcccag cacctgaccg
     4441 gtgccgagcg cctgccgtat acgctggcca tccatgccgg tggcggcgag aacccgcact
     4501 gccacctgat gatctccgag cggatcaatg acggcatcga gcggcccgcc gctcagtggt
     4561 tcaagcggta caacggcaag accccggaga agggcggggc acagaagacc gaagcgctca
     4621 agcccaaggc atggcttgag cagacccgcg aggcatgggc cgaccatgcc aaccgggcat
     4681 tagagcgggc tggccacgac gcccgcattg accacagaac acttgaggcg cagggcatcg
     4741 agcgcctgcc cggtgttcac ctggggccga acgtggtgga gatggaaggc cggggcatcc
     4801 gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc caacgcccag atcatcgact
     4861 tacaggaata ccgggaggca atagaccatg aacgcaatcg acagagtgaa gaaatccaga
     4921 ggcatcaacg agttagcgga gcagatcgaa ccgctggccc agagcatggc gacactggcc
     4981 gacgaagccc ggcaggtcat gagccagacc aagcaggcca gcgaggcgca ggcggcggag
     5041 tggctgaaag cccagcgcca gacaggggcg gcatgggtgg agctggccaa agagttgcgg
     5101 gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc ggagcgcgtc gcgggggtgg
     5161 cactggaagc tatggctaac cgtgatgctg gcttccatga tgcctacggt ggtgctgctg
     5221 atcgcatcgt tgctcttgct cgacctgacg ccactgacaa ccgaggacgg ctcgatctgg
     5281 ctgcgcttgg tggcccgatg aagaacgaca ggactttgca ggccataggc cgacagctca
     5341 aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga cgcacccacc ggccagatga
     5401 tgaaccggga atggtcagcc gccgaagtgc tccagaacac gccatggctc aagcggatga
     5461 atgcccaggg caatgacgtg tatatcaggc ccgccgagca ggagcggcat ggtctggtgc
     5521 tggtggacga cctcagcgag tttgacctgg atgacatgaa agccgagggc cgggagcctg
     5581 ccctggtagt ggaaaccagc ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg
     5641 caggcggtga acttcggggg cagattgccc ggacgctggc cagcgagtac gacgccgacc
     5701 cggccagcgc cgacagccgc cactatggcc gcttggcggg cttcaccaac cgcaaggaca
     5761 agcacaccac ccgcgccggt tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga
     5821 ccgccaccgc tggcccggcg ctggtgcagc aggctggcca gcagatcgag caggcccagc
     5881 ggcagcagga gaaggcccgc aggctggcca gcctcgaact gcccgagcgg cagcttagcc
     5941 gccaccggcg cacggcgctg gacgagtacc gcagcgagat ggccgggctg gtcaagcgct
     6001 tcggtcatga cctcagcaag tgcgacttta tcgccgcgca gaagctggcc agccggggcc
     6061 gcagtgccga ggaaatcggc aaggccatgg ccgaggccag cccagcgctg gcagagcgca
     6121 agcccggcca cgaagcggat tacatcgagc gcaccgtcag caaggtcatg ggtctgccca
     6181 gcgtccagct tgcgcgggcc gagctggcac gggcaccggc accccgccag cgaggcatgg
     6241 acaggggcgg gccagatttc agcatgtagt gcttgcgttg gtactcacgc ctgttatact
     6301 atgagtactc acgcacagaa gggggtttta tggaatacga aaaaagcgct tcagggtcgg
     6361 tctacctgat caaaagtgac aagggctatt ggttgcccgg tggctttggt tatacgtcaa
     6421 acaaggccga ggctggccgc ttttcagtcg ctgatatggc cagccttaac cttgacggct
     6481 gcaccttgtc cttgttccgc gaagacaagc ctttcggccc cggcaagttt ctcggtgact
     6541 gatatgaaag accaaaagga caagcagacc ggcgacctgc tggccagccc tgacgctgta
     6601 cgccaagcgc gatatgccga gcgcatgaag gccaaaggga tgcgtcagcg caagttctgg
     6661 ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg aagaactcag agcggcgcag
     6721 ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg cctgcaaagg aggcaatcaa
     6781 tggctaccca taagcctatc aatattctgg aggcgttcgc agcagcgccg ccaccgctgg
     6841 actacgtttt gcccaacatg gtggccggta cggtcggggc gctggtgtcg cccggtggtg
     6901 ccggtaaatc catgctggcc ctgcaactgg ccgcacagat tgcaggcggg ccggatctgc
     6961 tggaggtggg cgaactgccc accggcccgg tgatctacct gcccgccgaa gacccgccca
     7021 ccgccattca tcaccgcctg cacgcccttg gggcgcacct cagcgccgag gaacggcaag
     7081 ccgtggctga cggcctgctg atccagccgc tgatcggcag cctgcccaac atcatggccc
     7141 cggagtggtt cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg gtgctggaca
     7201 cgctgcgccg gttccacatc gaggaagaaa acgccagcgg ccccatggcc caggtcatcg
     7261 gtcgcatgga ggccatcgcc gccgataccg ggtgctctat cgtgttcctg caccatgcca
     7321 gcaagggcgc ggccatgatg ggcgcaggcg accagcagca ggccagccgg ggcagctcgg
     7381 tactggtcga taacatccgc tggcagtcct acctgtcgag catgaccagc gccgaggccg
     7441 aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt gtgagcaagg
     7501 ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc ggggtgctca
     7561 agcccgccgt gctggagagg cagcgcaaga gcaagggggt gccccgtggt gaagcctaag
     7621 aacaagcaca gcctcagcca cgtccggcac gacccggcgc actgtctggc ccccggcctg
     7681 ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac gtatgactac
     7741 ggcgacggca agcggatcga gttcagcggc ccggagccgc tgggcgctga tgatctgcgc
     7801 atcctgcaag ggctggtggc catggctggg cctaatggcc tagtgcttgg cccggaaccc
     7861 aagaccgaag gcggacggca gctccggctg ttcctggaac ccaagtggga ggccgtcacc
     7921 gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg caaaggaaat cggggcagag
     7981 gtcgatagtg gtggggcgct caagcacata caggactgca tcgagcgcct ttggaaggta
     8041 tccatcatcg cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc ggagtacgcc
     8101 agcgacgagg cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc gcaggccgtc
     8161 atgggtggcg gccagcatgt gcgcatcagc atggacgagg tgcgggcgct ggacagcgaa
     8221 accgcccgcc tgctgcacca gcggctgtgt ggctggatcg accccggcaa aaccggcaag
     8281 gcttccatag ataccttgtg cggctatgtc tggccgtcag aggccagtgg ttcgaccatg
     8341 cgcaagcgcc gcaagcgggt gcgcgaggcg ttgccggagc tggtcgcgct gggctggacg
     8401 gtaaccgagt tcgcggcggg caagtacgac atcacccggc ccaaggcggc aggctgaccc
     8461 cccccactct attgtaaaca agacattttt atcttttata ttcaatggct tattttcctg
     8521 ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat attttgaaaa
     8581 attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt tgcttccaga
     8641 tgtatgctct tctgctcccg aacgccagca agacgtagcc cagcgcgtcg gccagcttgc
     8701 aattcgcgct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8761 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8821 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     8881 cgcctggccc tgagagagtt gcagcaagcg gtccacgtgg tttgccccag caggcgaaaa
     8941 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     9001 cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     9061 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     9121 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     9181 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     9241 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     9301 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     9361 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     9421 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     9481 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     9541 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     9601 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     9661 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     9721 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     9781 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
     9841 tccgggcgct atcatgccat accgcgaaag gttttgcacc attcgatggt gtcaacgtaa
     9901 atgccgcttc gccttcgcgc gcgaattgca agctgatccg ggcttatcga ctgcacggtg
     9961 caccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta
    10021 aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg
    10081 ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatcgg
    10141 ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac taggcacccc
    10201 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    10261 ttcacacagg aaacagctat gaccatgatt acgaa
//
