LOCUS       V008644                 9065 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008644
VERSION     V008644
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Novel strategies for genomic manipulation of Trichoderma reesei with
            the purpose of strain engineering
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
   PUBMED   26150462
REFERENCE   2  (bases 1 to 9065)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien,
            Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE   3  (bases 1 to 9065)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2015) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2015) Institute of Chemical Engineering, TU Wien,
            Gumpendorfer Strasse 1a, Wien 1060, Austria"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        320..338
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4538..5680
                     /gene="ura3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Hypocrea
                     jecorina. Accession#: P21594"
     rep_origin      complement(7165..7753)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7930..8718)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(8938..9042)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccagcgg ccgccagatc tcgagatagt atctcattca gtaaaatact
      421 cgtacgagtt tgagggggcg cgaggctaag ctgcaagcgg gcgcggcaac actacctgta
      481 tgtattaata aagcagcaga gagagagaga gccctggact gccggcaatc ctgagaaggg
      541 gggatcgacc ggaccatctg cacgcagcag ggctgctgct gccaacatgg agagactaaa
      601 gatgggattg gtggtacata gcaagacgct tggatgcttg ttgacgggct gctccaataa
      661 gcaagctcac aagcagtgct ttgggggtcc gcacggacgg tcgagagggc tcggacggct
      721 gatattgcag acctggacga gtcagttgtg caggtaggca ctcgtatgtg tgtctttgtg
      781 cttgatcaac cttgagtaag tattattagg gtgatggctt aggtaggtag gttaggtagg
      841 tagtttaggt acagggaggt gaggagggga gacgtccagc tgccatatgc gccacttgca
      901 gcctcaactc tgcaaaagcc agccccgatc acggcatgtc gtacatactg taaggtagta
      961 cctagtagta gaacgaatgc taaaggtgca gagcaactaa cttgagcaaa accccccctc
     1021 cccccatcga gattgattgc cctctacgct gaaaatagtc tcgccagccg gcgacgctta
     1081 gccgtggcgc taccgcgaca tggcgcttgt tagcctgcgg tccaggttga tgcgaagcat
     1141 gtcgcagggc gggtctcgtg cgtgccgaca gccagcagag agacagaaga gagcgagaga
     1201 gcgagagagc gagagagaga gagagagaga aagacgcctc gggggtttgg ctggtggctt
     1261 cgatgcttgt ccgtgtcaag ggttcacgaa agacgggacg gctgagttgg tgactggcgg
     1321 cggtggcatc gaggctggag tccaggcaca tgcatggcgc aaggcatgtc aggccatgtc
     1381 gtgtcatgcc ccggcacagc tgaagggaat actactattg agagctgttc tccaaggcgt
     1441 caagcatgtg agcaaaaact cgagttgtct agactcgact cgacttgcgg ctgcagtaca
     1501 acaatacagt actactcgta cacgtatgta cagcaaggag caggtggatc agcaaagttg
     1561 catgataatg gactggaccg gcaacgctca aagttcgcct tgcagctccg gcatgaaggg
     1621 cagacccccg ccattattag cgatgactag ccgtggaggc cactttggct ttagtgattt
     1681 aaaccccgag attcatgctc gatgctctac aggtatgtat gtactagctc agctcagcct
     1741 tagtaggtag gtattgaagt ctggtggtgc gaggaggacc tgcaggtggg agttttgggg
     1801 gaaaaagatg atgctcacca actaaaataa atagtcaggc cacacacaca acctactgag
     1861 cagaaccacc acaaccagtg aagagctact ccggatggct cgagtttttc agcaagatac
     1921 tagtgaattc tggatccttt cgtgaccggg cttcaaacaa tgatgtgcga tggtgtggtt
     1981 cccggttggc ggagtctttg tctactttgg ttgtctgtcg caggtcggta gaccgcaaat
     2041 gagcaactga tggattgttg ccagcgatac tataattcac atggatggtc tttgcgatca
     2101 gtagctagtg agagagagag aacatctatc cacaatgtcg agtgtctatt agacatactc
     2161 cgagaataaa gtcaactgtg tctgtgatct aaagatcgat tcggcagtcg agtagcgtat
     2221 aacaactccg agtaccagca aaagcacgtc gtgacaggag caggctttgc caactgcgca
     2281 accttgcttg aatgaggata cacggggtgc aacatggctg tactgatcca tcgcaaccaa
     2341 aatttctgtt tatagatcaa gctggtagat tccaattact ccacctcttg cgcttctcca
     2401 tgacatgtaa gtgcacgtag gaaaccatac ccaaattgcc tacagctgcg gagcatgagc
     2461 ctatggcgat cagtctggtc atgttaacca gcctgtgctc tgacgttaat gcagaataga
     2521 aagccgcggt tgcaatgcaa atgatgatgc ctttgcagaa atggcttgct cgctgactga
     2581 taccagtaac acctttgctt ggccgtctag cgctgttgat tgtattcatc acaacctcgt
     2641 ctccctcctt tgggttgagc tctttggatg gctttccaaa cgttaatagc gctagtagta
     2701 gaaatggagg gtaaactgaa catcagtcaa aagacgtcga gacgcagata ctactagata
     2761 cctatcccag cgacggaaaa tggacaaggt gtcgccgccg ccaagctctc agatgcgcta
     2821 tcgtgcatga tgcctctcat caacaaacgg cgccagtgac gggctagccg tgggattgac
     2881 tcgagctcgc ctcttctttg tgcttttctc ctgtcgacat cccggcttgc gcttggacct
     2941 cgccgggacg tgcaaacgaa cacatcactt tcaaagagag tgtttgatgc tcacgctcgg
     3001 atgctccagc ccccccttgc ttgcttgtcc atcattgagg ctcgaagggg cagttgctcc
     3061 ccattccctc tgctccaaca ggaactgtaa ccgaaaaaaa agccatcctc cttcttcctc
     3121 ttcctcttcc gcctcctcct cttcctcctc ctactaatac ctactcctcc tctcctcatc
     3181 gactagactg acccccccgg ttgggcccct cgtcccgtct ccaacagagc accaccagac
     3241 aaagacccct gcccgcgcga atccagaccc ccccagcaat tccgggcctc gttgatcctc
     3301 ctctactgta gttgtacata catacctacc gactgcattg cattggtaca gctgcaggca
     3361 cttccaggca cggccaccaa attgcagcgg cccttgcttg cttgcttggt tcgagaacta
     3421 ggatctgtgt cttttgcctt gccttgtctt gtctgggttc ctgctcgtct gcggcaatcg
     3481 gaacgccgcc agtgcggtgc caagcaaacc agccaaggta ggtacctacc actaggcttc
     3541 ttttctcgtt gtctcactct ctcttttcct ctttgtcctc tcttatcccc atcttttctc
     3601 tctctctctg ctcctttcct aaccacttcc ctacctttct ctttttcctt ttcttgtcat
     3661 ctccatcttg gctgacgaaa aaggtctgac tgggtaggta ttatctggca gacttgtgtg
     3721 tatcattcac cctatttctg cttcatagta catgtactgt acctgaacgg ctcaaccgct
     3781 atttacgact cttatttttt tgtggcgttg gtcacgtttg ccagctgttg tccgtctttc
     3841 tagggctcct caaacttgac ctgaccgagc tccctttctg gacccggtgg gcttcacttc
     3901 cagctgctga gcgacctgag ccgaacatcc tcagtccttg tccagcgcaa ttcattttct
     3961 ttccttttct ttttttttat tcctttcttt acttttattc tctctttttc tcctcttcct
     4021 cttcttcttc tttctcctcc tcctccatat cctcactctc gtctccctca ttactaccct
     4081 ctcggctcct caggtccacc aaccctcccg cacccaaacc tctgccgctg aaacccattc
     4141 ggtggtcgcc gttttttttt tttttttttt ctcaccccca aagtcgcaat atcgggtatc
     4201 gccgccggca ttgaatcgcc ttctccgcta gcatcgacta ctgctgctct gctctcgttg
     4261 ccagcgctgc tccctagaat tttgaccagg ggacgagccc gacattaaag caactccctc
     4321 gcctcgagac gactcggatc gcacgaaatt ctcccaatcg ccgacagttc ctactcctct
     4381 tcctcccgca cggctgtcgc gcttccaacg tcattcgcac agcagaattg tgccatctct
     4441 ctcttttttt tccccccctc taaaccgcca caacggcacc ctaagggtta aactatccaa
     4501 ccagccgcag cctcagcctc tctcagcctc atcagccatg gcaccacacc cgacgctcaa
     4561 ggccaccttc gcggccagga gcgagacggc gacgcacccg ctgacggctt acctgttcaa
     4621 gctcatggac ctcaaggcgt ccaacctgtg cctgagcgcc gacgtgccga cagcgcgcga
     4681 gctgctgtac ctggccgaca agattggccc gtcgattgtc gtgctcaaga cgcactacga
     4741 catggtctcg ggctgggact tccacccgga gacgggcacg ggagcccagc tggcgtcgct
     4801 ggcgcgcaag cacggcttcc tcatcttcga ggaccgcaag tttggcgaca ttggccacac
     4861 cgtcgagctg cagtacacgg gcgggtcggc gcgcatcatc gactgggcgc acattgtcaa
     4921 cgtcaacatg gtgcccggca aggcgtcggt ggcctcgctg gcccagggcg ccaagcgctg
     4981 gctcgagcgc tacccctgcg aggtcaagac gtccgtcacc gtcggcacgc ccaccatgga
     5041 ctcgtttgac gacgacgccg actccaggga cgccgagccc gccggcgccg tcaacggcat
     5101 gggctccatt ggcgtcctgg acaagcccat ctactcgaac cggtccggcg acggccgcaa
     5161 gggcagcatc gtctccatca ccaccgtcac ccagcagtac gagtccgtct cctcgccccg
     5221 gttaacaaag gccatcgccg agggcgacga gtcgctcttc ccgggcatcg aggaggcgcc
     5281 gctgagccgc ggcctcctga tcctcgccca aatgtccagc cagggcaact tcatgaacaa
     5341 ggagtacacg caggcctgcg tcgaggccgc ccgggagcac aaggactttg tcatgggctt
     5401 catctcgcag gagacgctca acaccgagcc cgacgatgcc tttatccaca tgacgcccgg
     5461 ctgccagctg ccccccgaag acgaggacca gcagaccaac ggatcggtcg gtggagacgg
     5521 ccagggccag cagtacaaca cgccgcacaa gctgattggc atcgccggca gcgacattgc
     5581 cattgtgggc cggggcatcc tcaaggcctc agaccccgta gaggaggcag agcggtaccg
     5641 atcagcagcg tggaaagcct acaccgagag gctgctgcga taggggaggg aagggaagaa
     5701 agaagtaaag aaaggcattt agcaagaagg gggaaaaggg agggaggaca aacggagctg
     5761 agaaagagct cttgtccaaa gcccggcatc atagaatgca gctgtattta ggcgacctct
     5821 ttttccatct tgtcgatttt tgttatgacg taccagttgg gatgatggat gattgtaccc
     5881 cagctgcgat tgatgtgtat ctttgcatgc aacaacacgc gatggcggag gcgaactgca
     5941 cattggaagg ttcatatatg gtcctgacat atctggtgga tctggaagca tggaattgta
     6001 tttttgattt ggcatttgct tttgcgcgtg gagggaacat atcaccctcg ggcatttttc
     6061 atttggtagg atggtttgga tgcagttgtc gacgatatca gcttccatat atacggaatt
     6121 gttcaattga gtattacctg atggctattt gtgtcgatcc tttgtgacat tgacatgtga
     6181 tggcgcctgt acctctaaat gtatgcagcg taacatcttc agagcacggc ggcatgttct
     6241 cccctctaca atattcagat aagcagtact tgtgctgtga gaaacccatt gatcgctggt
     6301 ggcaaccaag gcgatatgtc tccaggagag gaaattgcac gatcattgat cgtttgtgct
     6361 actcgtttcg tgtccaagcg aacagagtat taatatatac cattatatca tatgcatcgt
     6421 ggagtcgtta ctatgcagcc taacctaagg tacctacaaa tcggcatccc ggagttaacg
     6481 cggaggctct catcgccggg cgtctccacc aattcgacat atcaatgcta atcgggcgca
     6541 aaggctccaa cgcggatgct ggtcgcattg aggttccgca ttccttttcc tccttccgac
     6601 cctctttctt acatctcact ctcacatccc gtttgccggc ccgctcccca ccgagacacg
     6661 aaacggacgg caacaatcaa ccgtcccccc ggaacagcga cctgacctcc cctcccccca
     6721 tatacgatta cgagctgaac gagacgagag agagagagag agagaagaag aagacagaga
     6781 agaggcatgg ccgacattga ggtggcagtc aacgtacggg ccgagcgggg cccgactaag
     6841 aagcggagac ggatcgtcat ctcgtgcacc gagtgccatc gaaggaagca aaaggtcagg
     6901 cttctttcct accaagaaac aaagtgaagc ttattaatta actggcctca tgggccttca
     6961 cttcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa catggtcata
     7021 gctgtttcct tgcgtattgg gcgctctccg cttcctcgct cactgactcg ctgcgctcgg
     7081 tcgttcgggt aaagcctggg gtgcctaatg agcaaaaggc cagcaaaagg ccaggaaccg
     7141 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7201 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7261 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7321 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7381 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7441 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7501 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7561 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7621 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7681 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7741 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7801 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7861 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7921 cagttattat ttacccacca ctttggtaat ttcgcctttc acgtaatgca caaattcttc
     7981 cagctgatcc gcacggctgg ccagacgatc ttcttcctgg cccagatacg cctgacgcgc
     8041 ttccagaatc accggctgat actgcgccgg cagacgttcc atggcccaat ccgccgccac
     8101 atctttcggc gcaattttgc cggtcaccgc gctataccaa atacggctca gggtcagcac
     8161 cacgttgcgt tcatcacccg cccaatccgg cgggctgttc cacagggtca gggtttcgtt
     8221 cagcgcttca aacaggtcct gttccggcac cggatcaaac agttcttccg ctgccggacc
     8281 aaccagcgcc acgctatgtt cacgcgcttt ggtcagcaga atggccagat cgatatcaat
     8341 ggtcgccggt tcaaaaatgc cggccagaat atcgttacgc tgccattcgc caaactgcag
     8401 ctcacgtttc gccggataac gccacggaat aatgtcatca tgcaccacga tggtcacttc
     8461 caccgcacgc agaatttcgc tttcacccgg gctcgcgctg gtttccagca gatcgttaat
     8521 cagcgcacga cgggtggttt catccagacg cacggtcacg gtcaccagca gatcaatatc
     8581 gctatgcggt ttcaggccgc catccaccgc gctgccatac agatgcacgg ccagcagggt
     8641 cggttccaga tgacgttcaa tcacgcccac cacttcagac agctgggtgc tcacttccgc
     8701 aatcaccgct tcacgcataa tgttcagttt ggttttcgga cggctgccct gctgggtcac
     8761 atcgttgctg ctccacagca tcaggcaacg gcccaccgca taacgcgcct gctgtttgct
     8821 cgcacgcggc atgctctgca cgccaaaaaa gcaatcatag cacgccataa acaccgccac
     8881 cgcaccatta ccgccgctac gttcggtcag ggtacggctc caattacggc tacgcatact
     8941 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     9001 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     9061 gccac
//
