LOCUS       V008645                 8293 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008645
VERSION     V008645
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Novel strategies for genomic manipulation of Trichoderma reesei with
            the purpose of strain engineering
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
   PUBMED   26150462
REFERENCE   2  (bases 1 to 8293)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien,
            Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE   3  (bases 1 to 8293)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8293)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2015) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-JUN-2015) Institute of Chemical Engineering, TU Wien,
            Gumpendorfer Strasse 1a, Wien 1060, Austria"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        320..338
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3766..4908
                     /gene="ura3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Hypocrea
                     jecorina. Accession#: P21594"
     rep_origin      complement(6393..6981)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7158..7946)
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(8166..8270)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccagcgg ccgccagatc tcgagatagt atctcattca gtaaaatact
      421 cgtacgagtt tgagggggcg cgaggctaag ctgcaagcgg gcgcggcaac actacctgta
      481 tgtattaata aagcagcaga gagagagaga gccctggact gccggcaatc ctgagaaggg
      541 gggatcgacc ggaccatctg cacgcagcag ggctgctgct gccaacatgg agagactaaa
      601 gatgggattg gtggtacata gcaagacgct tggatgcttg ttgacgggct gctccaataa
      661 gcaagctcac aagcagtgct ttgggggtcc gcacggacgg tcgagagggc tcggacggct
      721 gatattgcag acctggacga gtcagttgtg caggtaggca ctcgtatgtg tgtctttgtg
      781 cttgatcaac cttgagtaag tattattagg gtgatggctt aggtaggtag gttaggtagg
      841 tagtttaggt acagggaggt gaggagggga gacgtccagc tgccatatgc gccacttgca
      901 gcctcaactc tgcaaaagcc agccccgatc acggcatgtc gtacatactg taaggtagta
      961 cctagtagta gaacgaatgc taaaggtgca gagcaactaa cttgagcaaa accccccctc
     1021 cccccatcga gattgattgc cctctacgct gaaaatagtc tcgccagccg gcgacgctta
     1081 gccgtggcgc taccgcgaca tggcgcttgt tagcctgcgg tccaggttga tgcgaagcat
     1141 gtcgcagggc gggtctcgtg cgtgccgaca gccagcagag agacagaaga gagcgagaga
     1201 gcgagagagc gagagagaga gagagagaga aagacgcctc gggggtttgg ctggtggctt
     1261 cgatgcttgt ccgtgtcaag ggttcacgaa agacgggacg gctgagttgg tgactggcgg
     1321 cggtggcatc gaggctggag tccaggcaca tgcatggcgc aaggcatgtc aggccatgtc
     1381 gtgtcatgcc ccggcacagc tgaagggaat actactattg agagctgttc tccaaggcgt
     1441 caagcatgtg agcaaaaact cgagttgtct agactcgact cgacttgcgg ctgcagtaca
     1501 acaatacagt actactcgta cacgtatgta cagcaaggag caggtggatc agcaaagttg
     1561 catgataatg gactggaccg gcaacgctca aagttcgcct tgcagctccg gcatgaaggg
     1621 cagacccccg ccattattag cgatgactag ccgtggaggc cactttggct ttagtgattt
     1681 aaaccccgag attcatgctc gatgctctac aggtatgtat gtactagctc agctcagcct
     1741 tagtaggtag gtattgaagt ctggtggtgc gaggaggacc tgcaggtggg agttttgggg
     1801 gaaaaagatg atgctcacca actaaaataa atagtcaggc cacacacaca acctactgag
     1861 cagaaccacc acaaccagtg aagagctact ccggatggct cgagtttttc agcaagatac
     1921 tagtagtaga aatggagggt aaactgaaca tcagtcaaaa gacgtcgaga cgcagatact
     1981 actagatacc tatcccagcg acggaaaatg gacaaggtgt cgccgccgcc aagctctcag
     2041 atgcgctatc gtgcatgatg cctctcatca acaaacggcg ccagtgacgg gctagccgtg
     2101 ggattgactc gagctcgcct cttctttgtg cttttctcct gtcgacatcc cggcttgcgc
     2161 ttggacctcg ccgggacgtg caaacgaaca catcactttc aaagagagtg tttgatgctc
     2221 acgctcggat gctccagccc ccccttgctt gcttgtccat cattgaggct cgaaggggca
     2281 gttgctcccc attccctctg ctccaacagg aactgtaacc gaaaaaaaag ccatcctcct
     2341 tcttcctctt cctcttccgc ctcctcctct tcctcctcct actaatacct actcctcctc
     2401 tcctcatcga ctagactgac ccccccggtt gggcccctcg tcccgtctcc aacagagcac
     2461 caccagacaa agacccctgc ccgcgcgaat ccagaccccc ccagcaattc cgggcctcgt
     2521 tgatcctcct ctactgtagt tgtacataca tacctaccga ctgcattgca ttggtacagc
     2581 tgcaggcact tccaggcacg gccaccaaat tgcagcggcc cttgcttgct tgcttggttc
     2641 gagaactagg atctgtgtct tttgccttgc cttgtcttgt ctgggttcct gctcgtctgc
     2701 ggcaatcgga acgccgccag tgcggtgcca agcaaaccag ccaaggtagg tacctaccac
     2761 taggcttctt ttctcgttgt ctcactctct cttttcctct ttgtcctctc ttatccccat
     2821 cttttctctc tctctctgct cctttcctaa ccacttccct acctttctct ttttcctttt
     2881 cttgtcatct ccatcttggc tgacgaaaaa ggtctgactg ggtaggtatt atctggcaga
     2941 cttgtgtgta tcattcaccc tatttctgct tcatagtaca tgtactgtac ctgaacggct
     3001 caaccgctat ttacgactct tatttttttg tggcgttggt cacgtttgcc agctgttgtc
     3061 cgtctttcta gggctcctca aacttgacct gaccgagctc cctttctgga cccggtgggc
     3121 ttcacttcca gctgctgagc gacctgagcc gaacatcctc agtccttgtc cagcgcaatt
     3181 cattttcttt ccttttcttt ttttttattc ctttctttac ttttattctc tctttttctc
     3241 ctcttcctct tcttcttctt tctcctcctc ctccatatcc tcactctcgt ctccctcatt
     3301 actaccctct cggctcctca ggtccaccaa ccctcccgca cccaaacctc tgccgctgaa
     3361 acccattcgg tggtcgccgt tttttttttt ttttttttct cacccccaaa gtcgcaatat
     3421 cgggtatcgc cgccggcatt gaatcgcctt ctccgctagc atcgactact gctgctctgc
     3481 tctcgttgcc agcgctgctc cctagaattt tgaccagggg acgagcccga cattaaagca
     3541 actccctcgc ctcgagacga ctcggatcgc acgaaattct cccaatcgcc gacagttcct
     3601 actcctcttc ctcccgcacg gctgtcgcgc ttccaacgtc attcgcacag cagaattgtg
     3661 ccatctctct cttttttttc cccccctcta aaccgccaca acggcaccct aagggttaaa
     3721 ctatccaacc agccgcagcc tcagcctctc tcagcctcat cagccatggc accacacccg
     3781 acgctcaagg ccaccttcgc ggccaggagc gagacggcga cgcacccgct gacggcttac
     3841 ctgttcaagc tcatggacct caaggcgtcc aacctgtgcc tgagcgccga cgtgccgaca
     3901 gcgcgcgagc tgctgtacct ggccgacaag attggcccgt cgattgtcgt gctcaagacg
     3961 cactacgaca tggtctcggg ctgggacttc cacccggaga cgggcacggg agcccagctg
     4021 gcgtcgctgg cgcgcaagca cggcttcctc atcttcgagg accgcaagtt tggcgacatt
     4081 ggccacaccg tcgagctgca gtacacgggc gggtcggcgc gcatcatcga ctgggcgcac
     4141 attgtcaacg tcaacatggt gcccggcaag gcgtcggtgg cctcgctggc ccagggcgcc
     4201 aagcgctggc tcgagcgcta cccctgcgag gtcaagacgt ccgtcaccgt cggcacgccc
     4261 accatggact cgtttgacga cgacgccgac tccagggacg ccgagcccgc cggcgccgtc
     4321 aacggcatgg gctccattgg cgtcctggac aagcccatct actcgaaccg gtccggcgac
     4381 ggccgcaagg gcagcatcgt ctccatcacc accgtcaccc agcagtacga gtccgtctcc
     4441 tcgccccggt taacaaaggc catcgccgag ggcgacgagt cgctcttccc gggcatcgag
     4501 gaggcgccgc tgagccgcgg cctcctgatc ctcgcccaaa tgtccagcca gggcaacttc
     4561 atgaacaagg agtacacgca ggcctgcgtc gaggccgccc gggagcacaa ggactttgtc
     4621 atgggcttca tctcgcagga gacgctcaac accgagcccg acgatgcctt tatccacatg
     4681 acgcccggct gccagctgcc ccccgaagac gaggaccagc agaccaacgg atcggtcggt
     4741 ggagacggcc agggccagca gtacaacacg ccgcacaagc tgattggcat cgccggcagc
     4801 gacattgcca ttgtgggccg gggcatcctc aaggcctcag accccgtaga ggaggcagag
     4861 cggtaccgat cagcagcgtg gaaagcctac accgagaggc tgctgcgata ggggagggaa
     4921 gggaagaaag aagtaaagaa aggcatttag caagaagggg gaaaagggag ggaggacaaa
     4981 cggagctgag aaagagctct tgtccaaagc ccggcatcat agaatgcagc tgtatttagg
     5041 cgacctcttt ttccatcttg tcgatttttg ttatgacgta ccagttggga tgatggatga
     5101 ttgtacccca gctgcgattg atgtgtatct ttgcatgcaa caacacgcga tggcggaggc
     5161 gaactgcaca ttggaaggtt catatatggt cctgacatat ctggtggatc tggaagcatg
     5221 gaattgtatt tttgatttgg catttgcttt tgcgcgtgga gggaacatat caccctcggg
     5281 catttttcat ttggtaggat ggtttggatg cagttgtcga cgatatcagc ttccatatat
     5341 acggaattgt tcaattgagt attacctgat ggctatttgt gtcgatcctt tgtgacattg
     5401 acatgtgatg gcgcctgtac ctctaaatgt atgcagcgta acatcttcag agcacggcgg
     5461 catgttctcc cctctacaat attcagataa gcagtacttg tgctgtgaga aacccattga
     5521 tcgctggtgg caaccaaggc gatatgtctc caggagagga aattgcacga tcattgatcg
     5581 tttgtgctac tcgtttcgtg tccaagcgaa cagagtatta atatatacca ttatatcata
     5641 tgcatcgtgg agtcgttact atgcagccta acctaaggta cctacaaatc ggcatcccgg
     5701 agttaacgcg gaggctctca tcgccgggcg tctccaccaa ttcgacatat caatgctaat
     5761 cgggcgcaaa ggctccaacg cggatgctgg tcgcattgag gttccgcatt ccttttcctc
     5821 cttccgaccc tctttcttac atctcactct cacatcccgt ttgccggccc gctccccacc
     5881 gagacacgaa acggacggca acaatcaacc gtccccccgg aacagcgacc tgacctcccc
     5941 tccccccata tacgattacg agctgaacga gacgagagag agagagagag agaagaagaa
     6001 gacagagaag aggcatggcc gacattgagg tggcagtcaa cgtacgggcc gagcggggcc
     6061 cgactaagaa gcggagacgg atcgtcatct cgtgcaccga gtgccatcga aggaagcaaa
     6121 aggtcaggct tctttcctac caagaaacaa agtgaagctt attaattaac tggcctcatg
     6181 ggccttcact tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaaca
     6241 tggtcatagc tgtttccttg cgtattgggc gctctccgct tcctcgctca ctgactcgct
     6301 gcgctcggtc gttcgggtaa agcctggggt gcctaatgag caaaaggcca gcaaaaggcc
     6361 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     6421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     6481 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     6541 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6601 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6661 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6721 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6781 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6841 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6901 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6961 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7021 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7081 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7141 tggtctgaca gttattattt acccaccact ttggtaattt cgcctttcac gtaatgcaca
     7201 aattcttcca gctgatccgc acggctggcc agacgatctt cttcctggcc cagatacgcc
     7261 tgacgcgctt ccagaatcac cggctgatac tgcgccggca gacgttccat ggcccaatcc
     7321 gccgccacat ctttcggcgc aattttgccg gtcaccgcgc tataccaaat acggctcagg
     7381 gtcagcacca cgttgcgttc atcacccgcc caatccggcg ggctgttcca cagggtcagg
     7441 gtttcgttca gcgcttcaaa caggtcctgt tccggcaccg gatcaaacag ttcttccgct
     7501 gccggaccaa ccagcgccac gctatgttca cgcgctttgg tcagcagaat ggccagatcg
     7561 atatcaatgg tcgccggttc aaaaatgccg gccagaatat cgttacgctg ccattcgcca
     7621 aactgcagct cacgtttcgc cggataacgc cacggaataa tgtcatcatg caccacgatg
     7681 gtcacttcca ccgcacgcag aatttcgctt tcacccgggc tcgcgctggt ttccagcaga
     7741 tcgttaatca gcgcacgacg ggtggtttca tccagacgca cggtcacggt caccagcaga
     7801 tcaatatcgc tatgcggttt caggccgcca tccaccgcgc tgccatacag atgcacggcc
     7861 agcagggtcg gttccagatg acgttcaatc acgcccacca cttcagacag ctgggtgctc
     7921 acttccgcaa tcaccgcttc acgcataatg ttcagtttgg ttttcggacg gctgccctgc
     7981 tgggtcacat cgttgctgct ccacagcatc aggcaacggc ccaccgcata acgcgcctgc
     8041 tgtttgctcg cacgcggcat gctctgcacg ccaaaaaagc aatcatagca cgccataaac
     8101 accgccaccg caccattacc gccgctacgt tcggtcaggg tacggctcca attacggcta
     8161 cgcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8281 cgaaaagtgc cac
//
