LOCUS       40924_9346        5766 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Suicide vector pCD-RAsl1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Novel strategies for genomic manipulation of Trichoderma reesei with
            the purpose of strain engineering
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26150462
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE   3  (bases 1 to 5766)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3866..4454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4631..5419)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(5639..5743)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgagtg aaggccgtca
      361 aggcctaggc gcgccagcgg ccgcgaaata gaagacgaag acgagaggag gagagggtgc
      421 gagagggtgc gaaactgcgc ccttcagctc gctaattccg cccattacat tcagcgcaca
      481 cttggtggta catacatacc tacatacgta cccgtagttg tgcgaagcga gtcgagccag
      541 cggtcggatg tgccaatatg taccttgtag gtactcatga tagtcagcag gaagccgagg
      601 gatgatagca cgcacagcca gtctggtgtt tgtgctctgg attcagcccc tagattcact
      661 gaccaaaaga agcaagagaa gggaaaggga aaaggaagcc ctaaaaaaga gcagggttgt
      721 ccttcggcac caccccgaaa gcaacagcag tgatggacgg gactacgaca ctgtagtaca
      781 tacgccaacc caaagattcc gagttgcaat cattgccaca aaattgctcg actgtggcgg
      841 gagaggggaa agtctccgcg agggcatctc tgatgagtcc cgtgccacgc cgggtttctt
      901 gccttgaatc accacaaaat gtctcagcgc ggctcatcca tggccgcctt tttgggcgtt
      961 tcgtattact cttgggtttg actgtattat cggtcaagag ccaaaggcca ctcggctttt
     1021 ccgcccttgc ctctcttcca tttccccagg cgctgatagt atgatagttt cccctcattg
     1081 cagctttgga cgtcaatgcc aataactgta ttgtattgac aaatcatttg tccaaagacg
     1141 accgacacct ctgatatcac atacgagtag tagtacatac aatccccccc aaactccgcc
     1201 tttttggcgc gtgtgtgccg cgtagctcgt gcacaatgtc gggccgccgc cgccagcggc
     1261 cacttgctta gcaggaacct agtcagccgt ccttgtgtga ttaatatgca tgctagcgag
     1321 ctctccggag cccaaaatag aagcacccga attcagggcc tttttctcca caccaatcac
     1381 caaattcact gatttcaagg ttgttagccg cctcttatca ctaggtagac tagcacctaa
     1441 ttttaccgat atgtgtagtc catacatgta tgagatatac atcatgatct cgaagtaaaa
     1501 cccggtacga agaggtataa aagccgcgga acaacaactt gcagatgcca tcacgcaaga
     1561 cgcagacacg gctctcttct cgcgggcatc accagagccc aatcacctgg tcctcaggtg
     1621 gccacttgag cgccgtcaga atggccgcac agcagcagca gcatcaccac agatcttggc
     1681 ctcaactctt gatatttcgg cgagaataca attactagtc tagtctatgg aaattggatg
     1741 ttgtgcggct ctttggagac tggtagtaca taagccacga acatgatatt ggcggggaag
     1801 gtttagctgg aaattggaac aagtcagaga agtcgccgcg tattagcttg gtgttagtag
     1861 acacagcaag tcaagagtgg gagttagatt tctgaattga gtgacgcaga tggatgtagt
     1921 attcattatt gaagacagag cctttccatc aacgtgcaga gccatgaaat gcaatgacct
     1981 taaggagggg catctctccg ctttccatct gactgagata ctaacccgat tccatctcca
     2041 ttttctagca cacacatacc tggagtcata cctcaaccgg ccttcagtag ctaaacagct
     2101 atgtagcgca tccgcgctaa gccgcttagg cgataacgtt ggccggtgtc ggccatacaa
     2161 ttcttgccta tgactctgtc tcgactaact gattgaccaa cgtcaattcc ttatcaaagt
     2221 tttcgacctt gataactctc gatcttaatc tcgagctttt tatttcctca acatcattca
     2281 cagtaacgac tcaagccctt ggacttggcc agcaattgac acaaccacgc gccatctgct
     2341 ccattggcct catcgatccc atcgacttcg tcagcctcga caacgcatcc aacgcaatca
     2401 caaatacttc aacatggctg agaaaaaggc ggacgccccg gcagccggca tgctctgggg
     2461 tggcaggttc acaggttgca gcctcgcaaa tctagctgcg gattcagtat actgacctct
     2521 cgcgatgcgc aggtgcaatt gatcctctca tgcacaagta caatgcctcc atcgggtacg
     2581 acaaggccct gtacaaggaa gatatccttg gatccattgc ctttgcgagg gccaattcaa
     2641 aggctggcat catcagcgaa gacgaattcc aggcgatcga gcgtggcttg ctccaagtga
     2701 tggaagaatg gaagcagggc actttcgcca tcatgccgaa tgacgaagat gtgagagggc
     2761 acactcgatt ctaatctcaa acttctttcg aggctgacac aatggaccag attcatacgg
     2821 caaacgaacg tcgcctggga gaggtcatcg gcaaggacat cgcgggaaag cttcacacag
     2881 gtcgcagccg caacgagcaa gttgtctgtg atatgcgcat gtggcttcgc gatcggatcc
     2941 gcgaaatcga cagccaactc gttgctttcc tcaaggtcat tatcacacgt gccgaatctg
     3001 aaatgtaagc ccagccgtac ctttccagct aaatgccccc cccccccccc cccccctctc
     3061 ttactgacgg acaacctcag cgactacatc atgcccggat acacccatct ccagcgcgct
     3121 cagccagttc ggtggtctca atggctcatg tcctatgccg ctgcgttcaa gcaggatctc
     3181 gagcggttgc gtcaagtctt cgagagggtc aacctcagtc cacttggctg tggcgccttg
     3241 gccggcaatc cattcggaat cgaccgagac tcaatagctg cggagcttgg cttcagcgga
     3301 atcaccttga actccatgaa tacttcggcc gaccgcgatt tcctcgtcga tttcctgtcc
     3361 tggaactcca tcttcaccaa ccacgtcagc aggtgggctg aggacctcat catctactcg
     3421 acctctgagt tcagtttcgt ccgtctggcc gacgcctatt ccacgggcag ctctctcatg
     3481 cccaacaaat tcaacccgga ctcgttggag cttcttcgcg gcaagtcagg gcgtgctttc
     3541 ggccaaatgg ctggtttgat gatggtgagt cctcatatca aacatctcag ccagtttgac
     3601 ttggcttttg agggtgaccg cggccgccgt tccggtaatt aactggcctc atgggccttc
     3661 acttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta acatggtcat
     3721 agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc gctgcgctcg
     3781 gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag gccaggaacc
     3841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4501 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4561 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4621 acagttatta tttacccacc actttggtaa tttcgccttt cacgtaatgc acaaattctt
     4681 ccagctgatc cgcacggctg gccagacgat cttcttcctg gcccagatac gcctgacgcg
     4741 cttccagaat caccggctga tactgcgccg gcagacgttc catggcccaa tccgccgcca
     4801 catctttcgg cgcaattttg ccggtcaccg cgctatacca aatacggctc agggtcagca
     4861 ccacgttgcg ttcatcaccc gcccaatccg gcgggctgtt ccacagggtc agggtttcgt
     4921 tcagcgcttc aaacaggtcc tgttccggca ccggatcaaa cagttcttcc gctgccggac
     4981 caaccagcgc cacgctatgt tcacgcgctt tggtcagcag aatggccaga tcgatatcaa
     5041 tggtcgccgg ttcaaaaatg ccggccagaa tatcgttacg ctgccattcg ccaaactgca
     5101 gctcacgttt cgccggataa cgccacggaa taatgtcatc atgcaccacg atggtcactt
     5161 ccaccgcacg cagaatttcg ctttcacccg ggctcgcgct ggtttccagc agatcgttaa
     5221 tcagcgcacg acgggtggtt tcatccagac gcacggtcac ggtcaccagc agatcaatat
     5281 cgctatgcgg tttcaggccg ccatccaccg cgctgccata cagatgcacg gccagcaggg
     5341 tcggttccag atgacgttca atcacgccca ccacttcaga cagctgggtg ctcacttccg
     5401 caatcaccgc ttcacgcata atgttcagtt tggttttcgg acggctgccc tgctgggtca
     5461 catcgttgct gctccacagc atcaggcaac ggcccaccgc ataacgcgcc tgctgtttgc
     5521 tcgcacgcgg catgctctgc acgccaaaaa agcaatcata gcacgccata aacaccgcca
     5581 ccgcaccatt accgccgcta cgttcggtca gggtacggct ccaattacgg ctacgcatac
     5641 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5701 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5761 tgccac
//
