LOCUS       V008648                 7910 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008648
VERSION     V008648
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7910)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7910)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             933..3404
                     /gene="MALT1"
                     /label="Mucosa-associated lymphoid tissue lymphoma
                     translocation protein 1"
                     /note="Mucosa-associated lymphoid tissue lymphoma
                     translocation protein 1 from Homo sapiens. Accession#:
                     Q9UDY8"
     CDS             3414..3443
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3459..3476
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    3505..3729
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3775..4203
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4217..4546
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4613..5404
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5583..5716
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5753..5769)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5777..5793)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5801..5831)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5846..5867)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6155..6743)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6917..7774)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7775..7879)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatcctgcca ccatggcgct gttgggggac ccgctacagg
      961 ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc
     1021 tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg
     1081 agggccgggg ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca
     1141 gttgcctaga cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc
     1201 tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc
     1261 tgcaggctat ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta
     1321 ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg
     1381 caactggaca tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg
     1441 gaaatacatc agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct
     1501 gtcgagttaa taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg
     1561 acatcccaga gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct
     1621 gtgttgaacc aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg
     1681 ctgttggaag ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg
     1741 agaccaaaaa gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt
     1801 gtcatgtata taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa
     1861 gaacagatga ggcagtggag tgcactgaag atgaattaaa taatcttggt catcctgata
     1921 ataaagagca aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa
     1981 atatgaatta ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga
     2041 ctaacttact gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg
     2101 agatgcgtaa tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat
     2161 tatattatgc aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg
     2221 ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc
     2281 aagaaaaaga aactggactt aatgtgttct tattggatat ggcgcgcaaa agaaatgact
     2341 acgatgatac cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat
     2401 atgccacgtg tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa
     2461 tctttatgaa atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg
     2521 atgaagttgc agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga
     2581 ttcgaagtag tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt
     2641 ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta
     2701 tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca
     2761 atgtcatgat catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg
     2821 cctacgttac tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca
     2881 cgcctgaaga aactggcagc tacttggtat caaaggatct tcccaagcat tgcctctata
     2941 ccagactcag ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat
     3001 atcagtactc aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac
     3061 ctctcattgc taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt
     3121 gtcttatgtc taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt
     3181 atcactcatt gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta
     3241 atgttacacc agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt
     3301 tcgctcacca tgcttcatgt cattttagta gaagtaatgt gccagtagag acaactgatg
     3361 aaataccatt tagtttctct gacaggctca gaatttctga aaaagggccc ttcgaacaaa
     3421 aactcatctc agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgag
     3481 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3541 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3601 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3661 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3721 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3781 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3841 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3901 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3961 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4021 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4081 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4141 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4201 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4321 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4381 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4441 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4501 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4561 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     4621 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4681 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4741 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     4801 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4861 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4921 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4981 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5041 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5101 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5161 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5221 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5281 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5341 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5401 cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat
     5461 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5521 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5581 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5701 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     5761 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5821 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5881 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
