LOCUS       V008652                 7919 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008652
VERSION     V008652
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7919)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7919)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7919)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             923..946
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             953..997
                     /label="AviTag(TM)"
                     /note="peptide tag that allows for enzymatic biotinylation"
     CDS             1013..3484
                     /gene="MALT1"
                     /label="Mucosa-associated lymphoid tissue lymphoma
                     translocation protein 1"
                     /note="Mucosa-associated lymphoid tissue lymphoma
                     translocation protein 1 from Homo sapiens. Accession#:
                     Q9UDY8"
     polyA_signal    3516..3740
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3786..4214
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4228..4557
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4624..5415
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5592..5725
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5762..5778)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5786..5802)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5810..5840)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5855..5876)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6164..6752)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6926..7783)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7784..7888)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac caggcctgaa
      961 cgacatcttc gaggcccaga agatcgagtg gcacgaggag gatcctgcca ccatggcgct
     1021 gttgggggac ccgctacagg ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc
     1081 ccctccggcc ggcgcgaccc tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga
     1141 gctcctggat caggcgcccg agggccgggg ctggaggaga ctggcggagc tggcggggag
     1201 tcgcgggcgc ctccgcctca gttgcctaga cctggagcag tgttctctta aggtactgga
     1261 gcctgaagga agccccagcc tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt
     1321 cacagaattg agtgatttcc tgcaggctat ggaacacact gaagttcttc agcttctcag
     1381 ccccccagga ataaagatta ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt
     1441 tgtgaaactg tgttgccggg caactggaca tccttttgtt caatatcagt ggttcaaaat
     1501 gaataaagag attccaaatg gaaatacatc agagcttatt tttaatgcag tgcatgtaaa
     1561 agatgcaggc ttttatgtct gtcgagttaa taacaatttc acctttgaat tcagccagtg
     1621 gtcacagctg gatgtttgcg acatcccaga gagcttccag agaagtgttg atggcgtctc
     1681 tgaatccaag ttgcaaatct gtgttgaacc aacttcccaa aagctgatgc caggcagcac
     1741 attggtttta cagtgtgttg ctgttggaag ccctattcct cactaccagt ggttcaaaaa
     1801 tgaattacca ttaacacatg agaccaaaaa gctatacatg gtgccttatg tggatttgga
     1861 acaccaagga acctactggt gtcatgtata taatgatcga gacagtcaag atagcaagaa
     1921 ggtagaaatc atcataggaa gaacagatgc ggcagtggca tgcactgaag atgaattaaa
     1981 taatcttggt catcctgata ataaagagca aacaactgac cagcctttgg cgaaggacaa
     2041 ggttgccctt ttgataggaa atatgaatta ccgggagcac cccaagctca aagctccttt
     2101 ggtggatgtg tacgaattga ctaacttact gagacagctg gacttcaaag tggtttcact
     2161 gttggatctt actgaatatg agatgcgtaa tgctgtggat gagtttttac tccttttaga
     2221 caagggagta tatgggttat tatattatgc aggacatggt tatgaaaatt ttgggaacag
     2281 cttcatggtc cccgttgatg ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca
     2341 aaatatactg aaattgatgc aagaaaaaga aactggactt aatgtgttct tattggatat
     2401 gtgtaggaaa agaaatgact acgatgatac cattccaatc ttggatgcac taaaagtcac
     2461 cgccaatatt gtgtttggat atgccacgtg tcaaggagct gaagcttttg aaatccagca
     2521 ttctggattg gcaaatggaa tctttatgaa atttttaaaa gacagattat tagaagataa
     2581 gaaaatcact gtgttactgg atgaagttgc agaagatatg ggtaagtgtc accttaccaa
     2641 aggcaaacag gctctagaga ttcgaagtag tttatctgag aagagagcac ttactgatcc
     2701 aatacaggga acagaatatt ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc
     2761 tcatgaactt ccagaaagta tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg
     2821 atttgcagct gagttttcca atgtcatgat catctataca agtatagttt acaaaccacc
     2881 ggagataata atgtgtgatg cctacgttac tgattttcca cttgatctag atattgatcc
     2941 aaaagatgca aataaaggca cgcctgaagc aaccggtagc tacttggtat caaaggatct
     3001 tcccaagcat tgcctctata ccagactcag ttcactgcaa aaattaaagg aacatctagt
     3061 cttcacagta tgtttatcat atcagtactc aggattggaa gatactgtag aggacaagca
     3121 ggaagtgaat gttgggaaac ctctcattgc taaattagac atgcatcgag gtttgggaag
     3181 gaagacttgc tttcaaactt gtcttatgtc taatggtcct taccagagtt ctgcagccac
     3241 ctcaggagga gcagggcatt atcactcatt gcaagaccca ttccatggtg tttaccattc
     3301 acatcctggt aatccaagta atgttacacc agcagatagc tgtcattgca gccggactcc
     3361 agatgcattt atttcaagtt tcgctcacca tgcttcatgt cattttagta gaagtaatgt
     3421 gccagtcgcg acaactgatg aaataccatt tagtttctct gacaggctca gaatttctga
     3481 aaaagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     3541 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     3601 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     3661 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     3721 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     3781 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4141 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4201 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     4261 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     4321 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4381 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4441 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     4501 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4561 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     4621 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     4681 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     4741 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     4801 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     4861 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     4921 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     4981 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     5041 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     5101 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     5161 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     5221 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5281 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5341 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5401 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     5461 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     5521 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     5581 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5641 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5701 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     5761 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5821 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     5881 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     5941 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6001 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6061 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6121 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6181 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6241 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6301 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6361 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6421 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6481 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6541 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6601 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6661 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6721 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6781 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6841 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6901 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6961 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7021 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7081 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7141 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7201 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7261 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7321 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7381 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7441 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7501 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7561 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7621 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7681 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7741 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7801 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7861 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
