LOCUS       V008650                 8447 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V008650
VERSION     V008650
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8447)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8447)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             923..946
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     regulatory      962..971
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             968..1447
                     /codon_start=1
                     /note="unnamed protein product; HLH dimerization domain"
                     /protein_id="SJL87022.1"
                     /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSYAS
                     STPLHVPVPRALRMEEDSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFE
                     MNGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKPRILFSPFFHPGNSIHTQP"
     misc_feature    971..973
                     /label="Kozak"
                     /note="Kozak"
     CDS             1463..3934
                     /gene="MALT1"
                     /label="Mucosa-associated lymphoid tissue lymphoma
                     translocation protein 1"
                     /note="Mucosa-associated lymphoid tissue lymphoma
                     translocation protein 1 from Homo sapiens. Accession#:
                     Q9UDY8"
     misc_feature    3941..3964
                     /label="SGSSGSSG linker"
                     /note="SGSSGSSG linker"
     CDS             3965..4009
                     /label="AviTag(TM)"
                     /note="peptide tag that allows for enzymatic biotinylation"
     polyA_signal    4044..4268
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4314..4742
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4756..5085
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5152..5943
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    6120..6253
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6290..6306)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6314..6330)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6338..6368)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6383..6404)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6692..7280)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7454..8311)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8312..8416)
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc
      961 tgccaccatg gcgctgttgg gggacccgct acaggccctg ccgccctcgg ccgcccccac
     1021 ggggccgctg ctcgcccctc cggccggcgc gaccctcaac cgcctgcggg agccgctgct
     1081 gcggaggctc agctacgctt cctcgacgcc acttcatgtt ccagtgcctc gagcgctcag
     1141 gatggaggaa gactcgatcc gcctgcctgc gcacctgcgc ttgcagccaa tttactggag
     1201 cagggatgac gtagcccagt ggctcaagtg ggctgaaaat gagttttctt taaggccaat
     1261 tgacagcaac acgtttgaaa tgaatggcaa agctctcctg ctgctgacca aagaggactt
     1321 tcgctatcga tctcctcatt caggtgatgt gctctatgaa ctccttcagc atattctgaa
     1381 gcagaggaaa cctcggattc ttttttcacc attcttccac cctggaaact ctatacacac
     1441 acagccggtt gatcctgcca ccatggcgct gttgggggac ccgctacagg ccctgccgcc
     1501 ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc tcaaccgcct
     1561 gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg agggccgggg
     1621 ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca gttgcctaga
     1681 cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc tgtgtctgct
     1741 gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc tgcaggctat
     1801 ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta ctgtaaaccc
     1861 agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg caactggaca
     1921 tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg gaaatacatc
     1981 agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct gtcgagttaa
     2041 taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg acatcccaga
     2101 gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct gtgttgaacc
     2161 aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg ctgttggaag
     2221 ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg agaccaaaaa
     2281 gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt gtcatgtata
     2341 taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa gaacagatga
     2401 ggcagtggag tgcactgaag atgaattaaa taatcttggt catcctgata ataaagagca
     2461 aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa atatgaatta
     2521 ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga ctaacttact
     2581 gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg agatgcgtaa
     2641 tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat tatattatgc
     2701 aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg ctccaaatcc
     2761 atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc aagaaaaaga
     2821 aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact acgatgatac
     2881 cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat atgccacgtg
     2941 tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa tctttatgaa
     3001 atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg atgaagttgc
     3061 agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga ttcgaagtag
     3121 tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt ctgctgaatc
     3181 tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta tgtgtcttaa
     3241 gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca atgtcatgat
     3301 catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg cctacgttac
     3361 tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca cgcctgaaga
     3421 aactggcagc tacttggtat caaaggatct tcccaagcat tgcctctata ccagactcag
     3481 ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat atcagtactc
     3541 aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac ctctcattgc
     3601 taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt gtcttatgtc
     3661 taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt atcactcatt
     3721 gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta atgttacacc
     3781 agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt tcgctcacca
     3841 tgcttcatgt cattttagta gaagtaatgt gccagtagag acaactgatg aaataccatt
     3901 tagtttctct gacaggctca gaatttctga aaaagggccc agcggaagtt caggttcatc
     3961 cggaggcctg aacgacatct tcgaggccca gaagatcgag tggcacgagt aagggcccgt
     4021 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4081 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4141 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4201 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4261 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4321 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4381 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4441 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4501 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4561 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4621 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4681 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4741 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4801 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4861 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4921 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4981 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     5041 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     5101 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     5161 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5221 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5281 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5341 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5401 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5461 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5521 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5581 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5641 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5701 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5761 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5821 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5881 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5941 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6001 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6061 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6121 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6181 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6241 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6301 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6361 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6421 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6481 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6541 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6601 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7321 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7381 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7441 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7501 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7561 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7621 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7681 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7741 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7801 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7861 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7921 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7981 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8041 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8101 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8161 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8221 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8281 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8341 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8401 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
