LOCUS       40924_9306        5146 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Suicide vector pCD-EYFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Novel strategies for genomic manipulation of Trichoderma reesei with
            the purpose of strain engineering
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26150462
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE   3  (bases 1 to 5146)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5146)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      544..553
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1136..1852
                     /label=EYFP
                     /note="enhanced YFP"
     primer_bind     complement(2925..2941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2949..2965)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2973..3003)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3018..3039)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3327..3915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4089..4946)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4947..5051)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctcgagataa cggtgagact
      421 agcggccggt cccttatcca gctgtccacg ttggcctgcc ctcagttagc gctcaactca
      481 tgccctcact ggcgaggcga gggcaaggat ggaggggcag catcgcctga gttggagcaa
      541 agcgccgcca tgggagcagc gaaccaacgg agggatgccg tgctttgtcg tggctgctgt
      601 ggccaatccg ggcccttggt tggctcacag agcgttgctg tgagaccatg agctattatt
      661 gctaggtaca gtatagagag aggagagaga gagagagagt gtgagaggga aaaggtgagg
      721 ttgaagtgag gtagtaagtg aaaaaaaaaa aaaaatcaac actgacggct gccgtctgcc
      781 accctcctcc acccagccac ctgcacactc agcgcgcagc atcacctaat cttggctcgc
      841 cttccgcagc tcaggttgtt tttttttttt ctctctcctc gtcgaagccg cccttgttcc
      901 cttatttatt tccctctcct accttgtctg cctttggtcc atctgcccct ttgtctgcat
      961 ctcttttgca cgcatcgcct tatcgtcgtc tcttttttca ctcacgggag cttgacgaag
     1021 acctgactcg tgagcctcac ctgctgattt ctctcccccc ctcccgaccg gcttgacttt
     1081 tgtttctcct ccagtacctt atcgcgaagc cggaagaacc ctcttaacct ctagaatggt
     1141 gagcaagggc gaggagctgt tcaccggcgt cgtccccatc ctcgtcgagc tggacggcga
     1201 cgtcaacggc cacaagttca gcgtcagcgg cgagggcgag ggcgacgcca cctacggcaa
     1261 gctcaccctc aagttcatct gcacgaccgg caagctcccc gtcccctggc ccaccctcgt
     1321 caccaccttt ggctacggcc tccagtgctt cgcccgctac cccgaccaca tgaagcagca
     1381 cgactttttc aagagcgcca tgcccgaggg ctacgtccag gagcgcacca tctttttcaa
     1441 ggacgacggc aactacaaga cccgcgccga ggtcaagttc gagggcgaca ccctcgtcaa
     1501 ccgcatcgag ctgaagggca tcgactttaa ggaggacggc aacatcctcg gccacaagct
     1561 cgagtacaac tacaacagcc acaacgtcta catcatggcc gacaagcaga agaacggcat
     1621 caaggtcaac ttcaagatcc gccacaacat cgaggacggc agcgtccagc tggccgacca
     1681 ctaccagcag aacaccccca tcggcgacgg ccctgtcctc ctccccgaca accactacct
     1741 cagctaccag agcgccctca gcaaggaccc caacgagaag cgcgaccaca tggtcctgct
     1801 cgagttcgtc accgctgccg gcatcaccct cggcatggac gagctgtaca agtaagcggc
     1861 cggccgcatg catggctttc gtgaccgggc ttcaaacaat gatgtgcgat ggtgtggttc
     1921 ccggttggcg gagtctttgt ctactttggt tgtctgtcgc aggtcggtag accgcaaatg
     1981 agcaactgat ggattgttgc cagcgatact ataattcaca tggatggtct ttgcgatcag
     2041 tagctagtga gagagagaga acatctatcc acaatgtcga gtgtctatta gacatactcc
     2101 gagaataaag tcaactgtgt ctgtgatcta aagatcgatt cggcagtcga gtagcgtata
     2161 acaactccga gtaccagcaa aagcacgtcg tgacaggagc aggctttgcc aactgcgcaa
     2221 ccttgcttga atgaggatac acggggtgca acatggctgt actgatccat cgcaaccaaa
     2281 atttctgttt atagatcaag ctggtagatt ccaattactc cacctcttgc gcttctccat
     2341 gacatgtaag tgcacgtagg aaaccatacc caaattgcct acagctgcgg agcatgagcc
     2401 tatggcgatc agtctggtca tgttaaccag cctgtgctct gacgttaatg cagaatagaa
     2461 agccgcggtt gcaatgcaaa tgatgatgcc tttgcagaaa tggcttgctc gctgactgat
     2521 accagtaaca cctttgcttg gccgtctagc gctgttgatt gtattcatca caacctcgtc
     2581 tccctccttt gggttgagct ctttggatgg ctttccaaac gttaatagcg cgtttttctc
     2641 cacaaagtat tcgtatggac gcgcttttgg ctgtattgcg tgagctacca gcagcccaat
     2701 tggcgaagtc ttgagccgca ctcgcataga ataattgatt gcgcatttga tgcgattttt
     2761 gagcggctgt ttcaggcgac atttcgcccg cccttatttg ctccattata tcatcgacgg
     2821 catgtccaat agcccggtga tagtcttgtc gaatatggct gtcgtgaata acccatcggc
     2881 agcagatgat aatgattccg cagcacaagc ttggcgtaat catggtcata gctgtttcct
     2941 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3001 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3061 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3121 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3181 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3241 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3301 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3361 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3421 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3481 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     3541 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3601 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3661 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3721 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3781 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3841 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3901 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3961 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4021 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4081 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4141 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4201 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4261 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4321 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4381 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4441 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4501 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4561 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4621 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4681 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4741 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4801 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4861 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4921 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4981 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5041 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5101 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
