LOCUS       40924_9301        5942 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Suicide vector pCD-Dpyr4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Novel strategies for genomic manipulation of Trichoderma reesei with
            the purpose of strain engineering
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26150462
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE   3  (bases 1 to 5942)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3774..3804)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(3819..3840)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4131..4719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4893..5750)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5751..5855)
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat ctcgagatag tatctcattc
      361 agtaataata ctcgtacgag tttgaggggg cgcgaggcta agctgcaagc gggcgcggca
      421 acactacctg tatgtattaa taaagcagca gagagagaga gagccctgga ctgccggcaa
      481 tcctgagaag gggggatcga ccggaccatc tgcacgcagc agggctgctg ctgccaacat
      541 ggagagacta aagatgggat tggtggtaca tagcaagacg cttggatgct tgttgacggg
      601 ctgctccaat aagcaagctc acaagcagtg ctttgggggt ccgcacggac ggtcgagagg
      661 gctcggacgg ctgatattgc agacctggac gagtcagttg tgcaggtagg cactcgtatg
      721 tgtgtctttg tgcttgatca accttgagta agtattatta gggtgatggc ttaggtaggt
      781 aggttaggta ggtagtttag gtacagggag gtgaggaggg gagacgtcca gctgccatat
      841 gcgccacttg cagcctcaac tctgcaaaag ccagccccga tcacggcatg tcgtacatac
      901 tgtaaggtag tacctagtag tagaacgaat gctaaaggtg cagagcaact aacttgagca
      961 aaaccccccc tccccccatc gagattgatt gccctctacg ctgaaaatag tctcgccagc
     1021 cggcgacgct tagccgtggc gctaccgcga catggcgctt gttagcctgc ggtccaggtt
     1081 gatgcgaagc atgtcgcagg gcgggtctcg tgcgtgccga cagccagcag agagacagaa
     1141 gagagcgaga gagcgagaga gcgagagaga gagagagaga gaaagacgcc tcgggggttt
     1201 ggctggtggc ttcgatgctt gtccgtgtca agggttcacg aaagacggga cggctgagtt
     1261 ggtgactggc ggcggtggca tcgaggctgg agtccaggca catgcatggc gcaaggcatg
     1321 tcaggccatg tcgtgtcatg ccccggcaca gctgaaggga atactactat tgagagctgt
     1381 tctccaaggc gtcaagcatg tgagcaaaaa ctcgagttgt ctagactcga ctcgacttgc
     1441 ggctgcagta caacaataca gtactactcg tacacgtatg tacagcaagg agcaggtgga
     1501 tcagcaaagt tgcatgataa tggactggac cggcaacgct caaagttcgc cttgcagctc
     1561 cggcatgaag ggcagacccc cgccattatt agcgatgact agccgtggag gccactttgg
     1621 ctttagtgat ttaaaccccg agattcatgc tcgatgctct acaggtatgt atgtactagc
     1681 tcagctcagc cttagtaggt aggtattgaa gtctggtggt gcgaggagga cctgcaggtg
     1741 ggagttttgg gggaaaaaga tgatgctcac caactaaaat aaatagtcag gccacacaca
     1801 caacctactg agcagaacca ccacaaccag tgaagagcta ctccggatgg ctcgagtttt
     1861 tcagcaagat actagtgaga ggctgctgcg ataggggagg gaagggaaga aagaagtaaa
     1921 gaaaggcatt tagcaagaag ggggaaaagg gagggaggac aaacggagct gagaaagagc
     1981 tcttgtccaa agcccggcat catagaatgc agctgtattt aggcgacctc tttttccatc
     2041 ttgtcgattt ttgttatgac gtaccagttg ggatgatgga tgattgtacc ccagctgcga
     2101 ttgatgtgta tctttgcatg caacaacacg cgatggcgga ggcgaactgc acattggaag
     2161 gttcatatat ggtcctgaca tatctggtgg atctggaagc atggaattgt atttttgatt
     2221 tggcatttgc ttttgcgcgt ggagggaaca tatcaccctc gggcattttt catttggtag
     2281 gatggtttgg atgcagttgt cgacgatatc agcttccata tatacggaat tgttcaattg
     2341 agtattacct gatggctatt tgtgtcgatc ctttgtgaca ttgacatgtg atggcgcctg
     2401 tacctctaaa tgtatgcagc gtaacatctt cagagcacgg cggcatgttc tcccctctac
     2461 aatattcaga taagcagtac ttgtgctgtg agaaacccat tgatcgctgg tggcaaccaa
     2521 ggcgatatgt ctccaggaga ggaaattgca cgatcattga tcgtttgtgc tactcgtttc
     2581 gtgtccaagc gaacagagta ttaatatata ccattatatc atatgcatcg tggagtcgtt
     2641 actatgcagc ctaacctaag gtacctacaa atcggcatcc cggagttaac gcggaggctc
     2701 tcatcgccgg gcgtctccac caattcgaca tatcaatgct aatcgggcgc aaaggctcca
     2761 acgcggatgc tggtcgcatt gaggttccgc attccttttc ctccttccga ccctctttct
     2821 tacatctcac tctcacatcc cgtttgccgg cccgctcccc accgagacac gaaacggacg
     2881 gcaacaatca accgtccccc cggaacagcg acctgacctc ccctcccccc atatacgatt
     2941 acgagctgaa cgagacgaga gagagagaga gagagaagaa gaagacagag aagaggcatg
     3001 gccgacattg aggtggcagt caacgtacgg gccgagcggg gcccgactaa gaagcggaga
     3061 cggatcgtca tctcgtgcac cgagtgccat cgaaggaagc aaaaggtcag gcttctttcc
     3121 taccaagaaa caaagtgaag cttcacgatg ctgcatgggc attcgcacaa cagccctcgc
     3181 ctccgcctca aggtggatgg gaaataacat gaagatgatc acaccagcat gctgacacct
     3241 tactgttcat atccgcaaat cagtgcgatc gcgagctccc ctgcggcaac tgccggtcga
     3301 gaaacaagga agcccactgc gcctacgagg ccggcgcgcc caccgcccgc gagcacaacg
     3361 agacgcacgc ggccgcatct ttctagcgat gttcttcttt tattctctca agattttcag
     3421 gctgtatatt aaaacttata ttaagaacta tgctaaccac ctcatcagga accgttgtag
     3481 gtggcgtggg ttttcttggc aatcgactct catgaaaact acgagctaaa tattcaatat
     3541 gttcctcttg accaacttta ttctgcattt tttttgaacg aggtttagag caagcttcag
     3601 gaaactgaga caggaatttt attaaaaatt taaattttga agaaagttca gggttaatag
     3661 catccatttt ttgctttgca agttcctcag cattcttaac aaaagacgtc tcttttgaca
     3721 tgtttaaagt ttaaacctcc tgtgtgaaat tattatccgc tcataattcc acacattata
     3781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3841 attgcgttgc gctcactgcc aattgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3901 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4561 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4801 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4861 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4921 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4981 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5041 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5101 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5161 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5221 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5281 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5341 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5401 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5461 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5521 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5581 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5641 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5701 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5761 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5821 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5881 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     5941 cc
//
