LOCUS       40924_9296        7619 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Suicide vector pCD-Dhah1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7619)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Novel strategies for genomic manipulation of Trichoderma reesei with
            the purpose of strain engineering
  JOURNAL   Appl. Environ. Microbiol. (2015) In press
  PUBMED    26150462
REFERENCE   2  (bases 1 to 7619)
  AUTHORS   Derntl C, Kiesenhofer DP, Mach RL, Mach-Aigner AR.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria
REFERENCE   3  (bases 1 to 7619)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7619)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2015) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JUN-2015) Institute of Chemical Engineering, TU Wien, 
            Gumpendorfer Strasse 1a, Wien 1060, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1626..1635
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2218..3240
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(5451..5481)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(5496..5517)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5808..6396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6570..7427)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7428..7532)
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgcgagaa gatcttccag cgatatgcca
      361 ttggcctccc ccctgggaag acggtcaagg ccggcgactt catctcaatt gtacgtttgg
      421 cgatatctcc taccccgtgc agtccatggc taaacgactg atagatgccg caccgctgta
      481 tgacacacga caacagctat cctgtggcca ccaaggccat gaacctcggc ctcaagaaga
      541 tctacaatag cggccagatc gtcatgggta cggctctctc tcctcttact gtcaccgaga
      601 ccattctatt gacgtcaaca gctctcgacc acgatgtctc gaaccagtcc gagtccaacc
      661 tgcgcaaata caggctgatc gaggagtttg cccgcaagca cggaacatac ttctacccag
      721 ccggccacgg aatcgcccac cagatcatga tcgacgaagg tagagcaaca ttacccgaac
      781 caaaaagtgc taaatggaac acgctaacgg cagggttcaa ggccacgctt ggccagggac
      841 gctttgtgtc gccagtgatt cgcattcgac catctacggc ggtattggat gcctcggtac
      901 acccgtggta agatcagacg cagccggcat ctggagcgtt ggaagcacct ggcttcagca
      961 gccacctgtt gccaagatta ccttcacggg cctccttccc gttggcgtta ccggcaagga
     1021 cgtgattctg agtctctgct ccttgttcaa caacgacgag gtcctcaacc actgcgtgga
     1081 gtttgccgga tcagaacaga ccttggccag catccccatc gatgaccgcc tcaccatcag
     1141 caacatgacc acagaatggg gcgccttgag taattctaca cctttatccc tatgttgata
     1201 cgaagtggca acaaactgat cattggcctt gcaggtggat tgttccccgt cgatgacgcg
     1261 ctcatctcct ggtatcgcgg caaggcctcc gccgcagctg tgaccgacag ccctgccaag
     1321 gagcgcatca accacgagcg aatcgacgaa ctatcagaga accgactcgc cgctgaccca
     1381 ggcgctacct atgccaagga attctacctc aacttgtcca cattatcacc attcgtcact
     1441 gggcccaaca ctgtcaaggt tgccaaccct ttgagtaagc tgcataagat aacggtgaga
     1501 ctagcggccg gtcccttatc cagctgtcca cgttggcctg ccctcagtta gcgctcaact
     1561 catgccctca ctggcgaggc gagggcaagg atggaggggc agcatcgcct gagttggagc
     1621 aaagcgccgc catgggagca gcgaaccaac ggagggatgc cgtgctttgt cgtggctgct
     1681 gtggccaatc cgggcccttg gttggctcac agagcgttgc tgtgagacca tgagctatta
     1741 ttgctaggta cagtatagag agaggagaga gagagagaga gtgtgagagg gaaaaggtga
     1801 ggttgaagtg aggtagtaag tgaaaaaaaa aaaaaaatca acactgacgg ctgccgtctg
     1861 ccaccctcct ccacccagcc acctgcacac tcagcgcgca gcatcaccta atcttggctc
     1921 gccttccgca gctcaggttg tttttttttt ttctctctcc tcgtcgaagc cgcccttgtt
     1981 cccttattta tttccctctc ctaccttgtc tgcctttggt ccatctgccc ctttgtctgc
     2041 atctcttttg cacgcatcgc cttatcgtcg tctctttttt cactcacggg agcttgacga
     2101 agacctgact cgtgagcctc acctgctgat ttctctcccc ccctcccgac cggcttgact
     2161 tttgtttctc ctccagtacc ttatcgcgaa gccggaagaa ccctcttaac ctctagaatg
     2221 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     2281 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     2341 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     2401 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     2461 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     2521 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     2581 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     2641 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     2701 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     2761 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     2821 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     2881 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     2941 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     3001 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     3061 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     3121 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     3181 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     3241 taatgcatgg ctttcgtgac cgggcttcaa acaatgatgt gcgatggtgt ggttcccggt
     3301 tggcggagtc tttgtctact ttggttgtct gtcgcaggtc ggtagaccgc aaatgagcaa
     3361 ctgatggatt gttgccagcg atactataat tcacatggat ggtctttgcg atcagtagct
     3421 agtgagagag agagaacatc tatccacaat gtcgagtgtc tattagacat actccgagaa
     3481 taaagtcaac tgtgtctgtg atctaaagat cgattcggca gtcgagtagc gtataacaac
     3541 tccgagtacc agcaaaagca cgtcgtgaca ggagcaggct ttgccaactg cgcaaccttg
     3601 cttgaatgag gatacacggg gtgcaacatg gctgtactga tccatcgcaa ccaaaatttc
     3661 tgtttataga tcaagctggt agattccaat tactccacct cttgcgcttc tccatgacat
     3721 gtaagtgcac gtaggaaacc atacccaaat tgcctacagc tgcggagcat gagcctatgg
     3781 cgatcagtct ggtcatgtta accagcctgt gctctgacgt taatgcagaa tagaaagccg
     3841 cggttgcaat gcaaatgatg atgcctttgc agaaatggct tgctcgctga ctgataccag
     3901 taacaccttt gcttggccgt ctagcgctgt tgattgtatt catcacaacc tcgtctccct
     3961 cctttgggtt gagctctttg gatggctttc caaacgttaa tagcactagt tatgctgcag
     4021 tccggaggtt aagactaggt aattaaggca ggctaattta tttaaaaata gtattttctt
     4081 taatttctta ttaaatatat atataattat agctaggcta ggttatttta attatcttaa
     4141 actactaata ctagctaaat agctaaaata ataataacct ttttaataat attattatta
     4201 ttattattat tattaataat aatagtagta aaagctttta actattatta gtttatttat
     4261 aatctttata tagctagcta taatttaaaa tttataaata attaaaaaaa ggccttatta
     4321 ttactattac tattactatt attaatatat ttttagctat atccttaatt atattcttaa
     4381 ttataaaata aagctaaaag taaagtatta ttattttata ttttatatta taataagact
     4441 tactatataa atagatttaa ataatatact aaagtatatt aataaggtta ttaaaatact
     4501 taaggcctag cttataataa agctagtgtt ttttactttt tctttttatt attagcctaa
     4561 aggcttatta atattaataa cttactattt tttaaaattc ttaattaatt ataaaggttt
     4621 taaatagtat ttaaattata taagtaaagg agaggctttt ttaaatttta aaaaaaggta
     4681 aagttagtaa tattcttata gtaattagct aaagattata ctaatagctt ttctagctat
     4741 tattataaat aaatatcttt ttttttaaaa aacttactat tttttaactt cttaaagatt
     4801 atataattat aaaaaagttt atattatata taaaggttat taatataagg ctttaagcct
     4861 tttaataata aatttaagta taaaggcatt atagctatag taagataata ctgctttata
     4921 aaaaaataat agtaatatag ctaataatta aaagaatatt actaagccag ctttaattaa
     4981 taaagcgcta ctgctattat atttattatc tagctgatct ttctagaaga tctcctacaa
     5041 tattctcagc tgccatggaa aatcgatgtt cttcttttat tctctcaaga ttttcaggct
     5101 gtatattaaa acttatatta agaactatgc taaccacctc atcaggaacc gttgtaggtg
     5161 gcgtgggttt tcttggcaat cgactctcat gaaaactacg agctaaatat tcaatatgtt
     5221 cctcttgacc aactttattc tgcatttttt ttgaacgagg tttagagcaa gcttcaggaa
     5281 actgagacag gaattttatt aaaaatttaa attttgaaga aagttcaggg ttaatagcat
     5341 ccattttttg ctttgcaagt tcctcagcat tcttaacaaa agacgtctct tttgacatgt
     5401 ttaaagttta aacctcctgt gtgaaattat tatccgctca taattccaca cattatacga
     5461 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5521 gcgttgcgct cactgccaat tgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5581 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5641 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5701 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5761 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5821 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5881 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5941 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6001 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6061 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6121 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6181 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6241 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6301 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6361 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7501 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7561 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcc
//
